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According to the annual warning water levels from the Jinchengjiang Guaging Station on the Longjiang River, with 500 hPa circulation index, circulation characteristic amount, local climate and etc., analysis can be made with variance cycle, multiple linear regression, stepwise regression procedure.

1引言 龙江河流域发源于贵州省荔波县境内,流经桂西北地区中部,到达柳州市后流人柳江河,全长390公里。龙江河流域拥有10多个中小型梯级电站,具有发电、灌溉、航运、养殖等功能,是桂西北地区发展经济的主要河流。

Our laboratory also found a gene like Drosophila intersex in the testis of Cyprinus carpio. According to the significant function played by Drosophila ix in sex-determination, we are interested in the function of this gene in carp. To study the functions of cIX further, I cloned the full-length cDNA of cIX. The recombinant protein is produced and purified to serve as the antigen to induce antibody.

本实验室同样也在鲤鱼的睪丸中发现了类似Drosophila intersex的基因,鉴於intersex在果蝇中所扮演性别决定的重要功能,对於此基因在鲤鱼中所担任的功能而感到兴趣,为了进一步探讨cIX的功能,本论文完成cIX全长cDNA,设计并建构cIX的重组DNA,再利用纯化之重组蛋白诱导抗体。

The mRNA of pituitary glands from seven important economic Pleuronectiformes fishes was extracted,the cDNA sequences of GH gene were then isolated from Cleisthenes herzensteini,Limanda yokohamae,Pleuronichthys Cornutus,Cynoglossus robustus,Platichthys bicoloratus, Zebrias zebra and Paralichthys dentatus with RT-PCR method by using designed specific primers based on the reported GH cDNA sequences of Pleuronectiformes. The results of sequencing showed that lengths of the above cDNAs are as follows: 479 bp,564 bp,519 bp,440 bp,564 bp,440 bp and 522 bp.The GH cDNA sequences encode mature polypeptide of about 140 to 170 amino acid.The full-length growth hormone cDNAs of Paralichthys lethostigma and Scophthalmus maximus were cloned by Switching Mechanism At 5\' end of the RNA Transcript RACE technology.

从7种重要鲽形目经济鱼类——高眼鲽、黄盖鲽、木叶鲽、宽体舌鳎、石鲽和条鳎、夏鲆的脑垂体中提取mRNA,并采用RT-PCR方法,根据已克隆得到的鲆鲽鱼类生长激素基因的保守性序列设计特异性引物,克隆了含开放阅读框的生长激素(Growth Hormone,GH)cDNA序列,测序结果显示,高眼鲽、黄盖鲽、木叶鲽、宽体舌鳎、石鲽、条鳎和夏鲆的GH cDNA长度依次为479 bp、564 bp、519 bp、440 bp、564 bp、440 bp和522 bp,编码140-170个氨基酸的成熟GH多肽片段;从漠斑牙鲆和大菱鲆脑垂体中提取mRNA,利用SMART-RACE技术建立脑垂体cDNA文库,并从该文库中克隆出大菱鲆和漠斑牙鲆生长激素基因cDNA全长序列。

The total length of the coastline is about 1,566 kilometers.

海岸线全长大约 1,566 公里。

Japan 33,889 km long coastline.

日本海岸线全长33,889公里。

To elucidate the role of full-length functional AZ in T cell activation, we examined whether conditional expression of full-length AZ protein could elucidate the hallmarks of Jurkat T cell activation.

为了探讨全长具功能性的抑酶在T细胞活化过程中所扮演的角色,本论文研究抑酶的过表现对於Jurkat T细胞活化指标特性的影响。

Standing high on the tee one can view the small island surrounded by water, and the most challenging range lying on the left corner of the island with two sand pits connected in a complex manner, players can feel a sense of shock by all the scenes in front. They must master the strength and direction of the wind on that day to make sure of a good shot. The big and steep range makes the ball go faster and is a difficult test for players.

第九洞全长195码,标准杆数3,从高高的发球台向右前方望去四面环水的小岛上,最具挑战性的果岭斜卧在小岛左隅,前后两个沙坑相辅相成,相应成辉,虎视眈眈的与发球台遥相呼应,开阔的湖面泛着微光,令人望而生畏,要求球手们一定要掌握好当天风力的强度和风向,才能使球安全的落在球洞区上,大而陡的果岭使其速度更快,这又是考验球手的绝妙一笔。

Specific primers was designed based on conserver sequence of the other animals,firstly,total DNA was extracted from blood of Mongolian sheep and the BMP15 DNA was amplified by PCR,The purified-PCR.product was cloned into PMD18-T vector.

根据其他物种BMP15基因的保守序列设计特异性引物,扩增蒙古羊的全长DNA序列。从蒙古羊的血液中提取总DNA,采用PCR技术扩增出蒙古羊BMP15DNA序列。

A single examiner compared 102 sets of supine fluoroscopy images and full-length standing anteroposterior radiographs of the lower extremity to assess mechanical axis deviation and the joint line convergence angle.

一个单独的研究者对102张站立位全长前后位片和仰卧位透视片进行测量,得出MAX和JLCA。

The bioinformatics analysis showed that the gene had the highest homology with the gene of Penicillium janthinellum, which reached up to 76%. The first 26 amino acids might be a signal peptide sequence with the hydrophobicity of up to 2.63. The CBHⅠ gene consisted of the catalytic domain, convergence zone and cellulose-binding domain and its tertiary structure was mainly composed of 3-sheets.

分别扩增到鹅源草酸青霉F67纤维素酶CBHⅠ基因片段A(EU574736)、5'端序列B1(EU603295)、3'端序列B2(EU652768)及全长基因C(EU727171),并成功构建真核表达载体pPIC9K-CBHⅠ;生物信息学分析表明,该基因蛋白序列与微紫青霉氨基酸序列同源性最高,达76%,前26个氨基酸为信号肽序列,疏水性可达2.63,由催化功能域、衔接区和真菌性纤维素结合域构成,其三级结构主要为β-sheet。

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