全长

RESULTS: The activity of heparanase in the mammalian cell lysate detected was much higher than that in the culture medium. The molecular mass of the heparanase protein expressed in the transfected COS7 and CHO cells was about 53×103 by western blot. CONCLUSION: The fulllength heparanase gene is isolated.

结果：分离扩增出1629 bp的人HPA全长cDNA序列并构建了其真核表达载体；在转染后的COS7细胞和筛选出的CHO细胞裂解液中检测到较高的HPA活性和相对分子质量大小约53×103的目的蛋白免疫印迹表达带。

Jacket shall have a fulllength hinged access door with key lock.

外壳应该配置全长铰链监测门，并附带钥匙。

METHODS: Switch mechanism at 5′end of mRNA template technology, SMART technology, was used to construct the libraries. The purified Poly+RNA was used as template, Powerscript reverse transcriptase was used to transcribe and 5′oligo as a short extended template was added to the 5′ end of mRNA to enrich the fulllength cDNAs.

运用mRNA 5′末端的模板转换方法，即SMART技术，以纯化的Poly+RNA为模板，用Powerscript逆转录酶进行转录，并在mRNA 的5′末端添加一段5′ oligo作为延伸后的模板，从而富集全长 cDNA。

METHODS: Switch mechanism at 5′end of mRNA template technology, SMART technology, was used to construct the libraries. purified Poly+RNA was used as template, Powerscript reverse transcriptase was used to transcribe and 5′oligo as a short extended template was added to the 5′ end of mRNA to enrich the fulllength cDNAs.

运用mRNA 5′末端的模板转换方法，即SMART技术，以纯化的Poly+RNA为模板，用Powerscript逆转录酶进行转录，并在mRNA 的5′末端添加一段5′ oligo作为延伸后的模板，从而富集全长 cDNA。

METHODS摘要: Switch mechanism at 5′end of mRNA template technology, SMART technology, was used to construct the libraries. The purified Poly+RNA was used as template, Powerscript reverse transcriptase was used to transcribe and 5′oligo as a short extended template was added to the 5′ end of mRNA to enrich the fulllength cDNAs.

方法摘要：运用mRNA 5′末端的模板转换方法，即SMART技术，以纯化的Poly+RNA为模板，用Powerscript逆转录酶进行转录，并在mRNA 的5′末端添加一段5′ oligo作为延伸后的模板，从而富集全长 cDNA。

CONCLUSION摘要: Our successfully constructed cDNA libraries are fulllength libraries with high efficiency and can be screened by probes and antibodies to find the associated genes of the renal papillary adenocarcinoma.

结论摘要：我们构建的人肾乳头状腺癌及癌旁组织cDNA文库为高效、全长cDNA文库，可以用做探针、抗体等免疫学筛选，进一步探寻和肾乳头状腺癌相关的基因。

METHODS: Fulllength or fractions of VP22 were fused to C terminal of HBV core protein, and cloned into pcDNA3.1 vector, yielding eukaryotic expression plasmids of DN mutant.

将VP22全长及其不同区段融合于HBV核心蛋白的C端，克隆入pcDNA3.1构成DN突变体真核表达质粒。

Methods Many bioinformatics methods were used to analyses and predict the function of EOLA1 based on the fulllength cDNA of EOLA1.Results We didn't find any human protein high homologous with EOLA1 by homology comparison on line.

基于EOLA1全长cDNA序列，应用生物信息方法，从序列比对、染色体定位、基因结构分析、编码蛋白理化性质分析、蛋白质位点和序列模式预测等几个方面对EOLA1进行分析。

The successful cloning of fulllength gG2 of HSV2 and a successful forecast to B cell epitope of gG2 provide evidences and references for developing diagnostic kits for herpes virus infection and finding better target sites of HSV2 vaccine.

成功构建单纯疱疹病毒2型（HSV2）gG2全长基因克隆，为单纯疱疹病毒感染的诊断试剂的研发及抗单纯疱疹病毒疫苗研究寻找更好的靶位点提供研究材料和参考。

The successful cloning of fulllength gG2 of HSV2 and a successful forecast to B cell epitope of gG2 provide evidences and references for developing diagnostic kits for herpes virus infection and finding target sites of HSV2 vaccine.

成功构建单纯疱疹病毒2型（HSV2）gG2全长基因克隆，为单纯疱疹病毒感染的诊断试剂的研发及抗单纯疱疹病毒疫苗寻找更好的靶位点提供研究材料和。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。