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These results suggested that the function of each function domain in HIV 1 LTR exhibited distinct characteristics on gag gene expression in vaccinia virus:(1) The function of intact LTR on gag gene expression varied with the upstream poxvirus promoter;(2) NR sequence neither decreased nor increased the Gag protein exprssion level;(3) Enhancer sequence might not be recognized by recombinant vaccinia virus expression system;(4) TAR sequence enhanced Gag protein expression effectively;(5) U5 region and its downstream non translated sequence had little effect on

免疫印迹和免疫酶试验检测均表明,6株重组病毒的Gag蛋白表达量因LTR不同而有明显差异,表明HIV1的LTR及其下游基因置于痘病毒启动子控制下,在痘苗病毒中表达时有下述特点:(1)不同的痘苗病毒启动子与全长LTR相互作用,对gag基因表达有显著不同的调控效果;(2)NR序列对Gag蛋白表达没有明显影响;(3)EN序列不能被重组痘苗病毒表达系统识别;(4)TAR序列可提高Gag蛋白的表达量;(5)U5区及下游非翻译序列不影响Gag蛋白的表达。

Currently, Kaifeng municipal government has appointed Kaifeng Development Investment Co.Ltd to carry out the development and construction of the "giant water system tourist special region" program. The program stretches as long as 1300 meters, from the north Qingming Shanghe garden to the south Baogong Lake, covering an area of 140 acres. The construction includes new houses, roads, drainage, river, bridge, plantation, lamps, riverside landscapes and parergons, etc.

目前,根据开封市政府指示,由开封市发展投资有限公司具体负责开封市大型水系旅游特区工程项目的开发、建设,该项目水系工程北起清明上河园,南至包公湖,全长1300米,占地140亩,建设内容包括拆迁、道路、排水、河道、桥梁、绿化、路灯、沿岸景观、小品等。

New laccase gene and its full-length cDNA were clone from genomic library and equalized cDNA library of Ganoderma lucidum. Similarity between the sequence and other laccase gene were analyszed with NCBI blast, the results show that the new gene belong to multicopper oxidases gene family and have 60-70% Similarity with other laccase gene.

从灵芝的基因组文库和cDNA均一化文库中克隆了灵芝的漆酶基因和全长cDNA,并通过NCBI Conserved Domain Search氨基酸序列的相似性分析及与其它真菌漆酶之间的相似性分析,证明得到的蛋白是一种新的multicopper oxidases,其蛋白质序列与其它已克隆漆酶基因的同源性在6 0~7 0%之间,并对论文克隆的漆酶基因的启动子和终止子进行了分析。

Immunoassay indicated that protein expressed by full-length and truncated gene have the same immnogeneticity as the natural crystal protein.

免疫检测表明无论全长和截短基因的表达蛋白均和天然晶体蛋白具有相同的免疫原性。

Therefore,itwas more reasonable to consider yak,cattle and zebu as independent species of thegenus Bos,while buffalo belongs to another category.(6)The results indicate that the yak HSP72 gene was an intron-free 1926bpnucleotide,encoding a protein of 641 AAs.

6牦牛HSP72基因序列全长为1926bp,无内含子,共编码641个氨基酸;牦牛HSP72基因和HSP72蛋白与普通牛、猪、人相比存在着一定差异。

Invest Hong Kong also participated in the BT Global Challenge Yacht Race 2000-2001 as a sponsor of the Spirit of Hong Kong, a 72-foot racing yacht built in the Mainland by a Hong Kong company.

投资推广署又在二零零零至零一年度的英国电讯环球航海大赛中,赞助一艘名为&香港精神&号的帆船参赛。该艘帆船全长72尺,由一家香港公司在内地制造。

North-south from the Great Wall Wing Compass feng, north to Cape Mount the foot of dry gate 10 sets a total length of more than 3 kilometers.

北翼长城南起北斗峰,北至角山山麓的旱门10号台,全长3公里多。

Shanhaiguan is now the territory of the Great Wall is 26 kilometers length, including: the old leading the Great Wall, Great Wall, South Wing, Guan City, Great Wall, the north wing of the Great Wall, Great Wall, Cape Mount, three off the Great Wall and the nine other locations outside the Great Wall.

现属山海关境内的长城全长26公里,主要包括:老龙头长城、南翼长城、关城长城、北翼长城、角山长城、三道关长城及九门口长城等地段。

She wears a full-length gown of deep red chiffon with a red\/green iridescent velvet bodice and green velvet leaves featuring an embossed leaf print pattern.

她总是穿全长深红色礼服的薄纱与红\/绿虹彩天鹅绒紧身胸衣和绿色丝绒叶片功能的浮雕叶打印模式。

Lower for two-rail bridge, length 672 meters and 14 meters wide.

下层为双线铁路桥,全长672米,宽14米。

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在对危险的南部地区访问时,他斥责什叶派民兵领导人对中央集权的挑衅行为。

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