全长

BUTCH:"Worst... The worst was also the funnest,'cause I got up early Thanksgiving day and we went to the Burwick Marathon, but it's a nine-mile road race. Just a crusher."

巴奇：最糟糕的……最糟糕的一次也是最有趣的一次，因为感恩节那天我起床很早，我们去搞柏威克马拉松比赛，但全长只有9米远，一下子就跑完了。

Only 129 markers were used to construct the molecular genetic linkage map, and mapped on 21 chromosomes by Mapmaker 3.0 and Mapdraw V2.1. The map spaned about 2106.2 cM in wheat genome. The average distance of markers was 16.32 cM.

用Mapmaker 3.0和Mapdraw V2.1软件将129个SSR位点绘在小麦遗传连锁图上，该图谱覆盖小麦基因组全长2106.2 cM，标记间的平均遗传距离为16.32 cM。

In this study, we have cloned and identified two full-length cDNAs of OsBIMK2 and OsBISERK1, which encoding MAPK and SERK, respectively, and associated with benzothiadiazole induced resistance.

本文克隆和鉴定了水稻中与苯并噻二唑(benzothiadiazole，BTH)诱导抗病性相关的编码MAPK的OsBIMK2基因和编码SERK的OsBISERK1基因全长cDNA；研究了OsBIMK2基因和OsBISERK1基因在水稻—稻瘟病菌亲和与非亲和互作过程中的表达模式；原核表达了OsBIMK2基因，获得重组OsBIMK2蛋白，并研究了重组OsBIMK2蛋白的生化特性；克隆了OsBIMK2基因的启动子序列，并运用农杆菌介导的瞬间表达系统分析鉴定了启动子中参与基因表达调控的可能顺式元件；将水稻OsBIMK2基因导入烟草，获得过量表达OsBIMK2基因的转基因植株，这些转基因植株中防卫反应基因PR-1组成型表达，并提高了抗病性。

By sanding, closed, putty, primer, paint and other components 14, 32 meters in length, from painting to dry all the time to complete the process.

由砂磨、封闭、腻子、底漆、面漆等14台设备组成，全长32米，从涂漆到干燥所有的工序一次完成。

The 1 095 bp fragment of f3h gene of anthocyanin biosynthesis was cloned from fruit peel of riped Red Bartllet pear by RT PCR method.

利用 RT-PCR方法从红巴梨成熟果皮中克隆出花青素生成相关基因 f3 h的全长片段(1 0 95 bp)。

Objective To clone full length cDNA of human B7.1 and construct its retrovirus expressing vector.

目的克隆人B7.1全长cDNA及构建相应的逆转录病毒表达载体。

No immunostaining was seen in RPE. Double staining that Kir2. 1 and glutamine synthetase, Kir2. 1 and glutamine synthetase colocalized well. Intense Kir7. 1 immunolabeling was present on the apical surface of all RPE cells and appeared to extend over the length of the apical processes. Na〓, K〓-ATPase expression varied among RPE cells, but in highly expressing cells, it co-localized with Kir7. 1. Immunoreactivity of Kir2. 1、Kir4. 1 and Kir7. 1 acomplished by ABC immunohistochemistry in monkey and human retinae got the nearly same results as bovine.

间接免疫荧光组织化学显示，Kir2.1蛋白主要分布在Müller细胞与神经元相接触的细胞膜区域；与Müller细胞的特异性标记蛋白质抗谷氨酸合成酶抗体双重标记显示其分布特性重叠，在RPE未检测Kir2.1蛋白的免疫活性存在；Kir4.1蛋白集中分布于Müller细胞的内侧突起，与GS蛋白双重标记显示其分布特性重叠，RPE未检测Kir4.1蛋白的免疫活性存在；Kir7.1主要分布于RPE游离面及其突起的全长，与特异性标记RPE突起的Ezrin蛋白完全重叠，Na〓-K〓ATP酶在不同部位的RPE表达不同，Na〓-K〓ATP高表达的RPE细胞与Kir7.1的分布特性重叠。

The 329 bp terminal inverted repeat sequence can't form the conserved fold-back secondary structure as that of many Streptomyces linear replicons. Lacking of typical Streptomyces tap/tpg locus for te-lomere replication, pPR2.3c encodes a protein with two domains resembling the telomere associated protein of Strepto-myces and helicase of Haemophilus respectively. No typical Streptomyces iteron-rep locus for replication from the cen-trally located origin, two DNA fragments containing almost all pPR2 were cloned and introduced by transformation into S.

其端粒末端反向重复序列的长度为329 bp，不能像多数链霉菌的线型质粒那样能形成保守的&折返&的二级结构。pPR2虽然没有参与链霉菌端粒复制的保守的tap/tpg基因，但是pPR2.3c基因编码了一个双结构域蛋白，分别同链霉菌的端粒复制相关蛋白Tap和嗜血杆菌的解旋酶具有相似性。pPR2缺少典型的链霉菌重复序列-复制基因区段，将几乎覆盖全长pPR2的两段DNA进行克隆后，不能转化变铅青链霉菌。

Chimeric clone strategy has proved to be successful in rescuing CRF07_BC proviral genome,which was a straightforward,feasible and highly efficent method.

LTR功能和全长克隆策略对感染性克隆构建至关重要，pNL4-3的LTR能成功用于拯救CRF07_BC毒株前病毒基因组。

Expression vectors pET-TPO and pBV-TPO were constructed to express full-length TPO cDNA in E.coli . Influence of SD-AUG and promotors on the expression level was studied.

在克隆并分析了TPO cDNA的基础上，将全长hTPO cDNA克隆到原核表达载体pET-15b和pBV220中得到了表达，并且以pBV220为载体探讨了SD序列与AUG之间的距离对cDNA表达效率的影响；为了进一步提高表达效率，将hTPO cDNA 5？

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。