全长

Nine shelter bridge 680 of total length of quay this, support by 519 stake, bank being winding walking, visit it while entering the sea directly.

该码头的&九曲廊桥&全长680米，由519根木桩支撑，自岸边逶迤而行，直探进海中。

The highway, 414 miles (666 kilometers) of gravel known as the Haul Road, was built to haul construction crews to the Prudhoe Bay oil fields.

高速公路全长666公里沙砾路面，建筑的目的是运输建筑材料到普拉德霍湾油田。

Originally, the fourth day's tour was planned to ride from Tainan to Fong Bi Tou Harbor, a 71-kilometer ride. However, because the riders felt stronger and more used to long distance riding after the first three days' cycling accomplishments, they decided to change today's finishing point to Dapeng Bay National Scenic Area in Donggang Township, an 82-kilometer ride.

进入第四天的A-Team自行车环岛行程，由台南市出发，原订终点为凤鼻头渔港，全长71公里，不过考量到车队成员们经过3天的磨练之后，体能逐渐上升，也习惯了长途的骑乘，71公里的路途稍嫌太短，於是临时将当天的终点拉远至东港的大鹏湾国家风景区，全程共82公里。

EST sequence of LDH-C gene from black-lipped pika was cloned by PCR with degenerate primers; then the full length of open reading frame and 3'UTR sequence were cloned by RACE technique.

方法］采用简并引物PCR克隆得到黑唇鼠兔LDH-C基因的EST序列，再根据EST序列采用RACE技术克隆出基因的开放阅读框全长，和3'UTR序列。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Adopt pair of blueprint paper and budgetary estimate to have review a case in which a death sentence has been passed by a lower court, do well visa of spot of invite public bidding of engineering construction team, control and project allocate funds wait for measure to reduce project cost, manage through strict quality, undertake reforming innovating, raise the method such as service quality to improve project quality.

大庆油田设计院通过总承包全长为8.352km的北二一四路公路建设工程，对这一新模式进行实践探索。采取对设计图纸和概算进行复核、搞好工程施工队伍招标、控制现场签证和工程拨款等措施来降低工程成本，通过严格质量治理、进行改革创新、提高服务质量等手段来提高工程质量。

The replacement of woven screen, they should ensure that both sides of the plate and mesh sieve box hook equal to the gap between the first fastening the middle pressed flat, while taut tensioning plate to maintain uniform mesh surface tension, and hand - hammer gently tap along the length, check the tension the situation.

12更换编织筛网时，应保证筛箱两侧板与筛网钩子之间有相等的间隙，先紧固中间压紧扁钢，同时拉紧张紧板保持筛网表面张力均匀，并用手锤沿全长轻轻敲打，检查张紧情况。

At 2.2 km the bridge is the world's longest combined road and rail suspension bridge.

版主译文：青马大桥全长2.2公里，是世界上最长的组合公路拉线桥。

The DNA component 5 (DNA-5) of Banana bunchy top virus NS strain was cloned and sequenced. It was 1014nts in full length and could code for 146 amino acids.

本文利用 PCR 技术扩增得到香蕉束顶病毒NS 株 DNA 组分 5 的全基因，该基因全长为 1014nt，具有一个开放阅读框，编码 146 个氨基酸，蛋白质二级结构包括 6 个α螺旋，7 个β折叠。

An analysis of the effect of tooth path and optimum clearance angle on tool life in the up milling and down milling processes is presented.

就铣削轨迹长度而论，在相同的切削情况下顺铣的轨迹较短，但其差量甚小，仅占全长百分之几。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。