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The cDNA is 987 base pairs of length and encodes a protein of 177 amino acid residues.

该cDNA全长987bp编码一个177氨基酸的蛋白,比较NM23-H1蛋白,NM23-H1B在氨基端多了额外的25个氨基酸。

Such studies are highly dependent on the efficient in vitro GLUT expression and, thus far, there has been no report of a system for high expression of GLUTs in vitro.

本实验利用分子生物学技术,获得了人类GLUT家族GLUT1、GLUT2和GLUT3三个家族成员的全长基因,构建了pESP-GLUTs表达载体,实现了GST-GLUT1、GST-GLUT2、GST-GLUT3融合蛋白在粟酒裂殖酵母中的高效表达。

An identical nucleotide sequence in various passages was obtained by analysis using DNASTAR software, to which the homologies of nucleotides of E2 gene of passages 14, 16, 17, 18 and 23 were 100%, 100%, 99.8%, 99.8%and 99.6%, and those of amino acids were 100%, 100%, 100%, 99.3%and 99.2%, respectively.

结果 Matsuba株E2基因全长846bp,传至23代时仍有很高的同源性,第14、16、17、18和23代与一致序列的核苷酸同源性分别为100%、100%、99.8%、99.8%和99.6%,氨基酸同源性分别为100%、100%、100%、99.3%和99.2%。

Methods: From January 2004 to September 2006, 56 laparoscopic nephrectomies were performed. Hem-o-lok clips were used to control renal artery and vein in 38 cases, including 9 simple nephrectomies, 18 radical nephrectomies and 11 nephroureterectomies.

2004年1月至2006年9月我科行腹腔镜肾切除术56例,其中38例术中应用Hem-o-lok夹处理肾蒂血管,包括腹腔镜单纯肾切除9例,腹腔镜肾癌根治术18例,腹腔镜肾输尿管全长切除术11例。

Blade is 31.1/2".The pommels decorated with bulging images of hammer and sickle, five-pointed star and abbreviation "CCCP.

全长39,刃长31.1/2。柄首刻镰刀锤子,五星及俄语&苏联&CCCP的图案。

Lots of offshore construction methods have been adopted, such as erecting of the upper pylon shafts and main girders with huge floating cranes, prefabricating of the caissons in docks and floating of the caissons to their destinations.

里翁-安蒂里翁大桥采用斜拉桥主桥和两侧引桥组成的方案,其中主桥采用五跨连续结构,跨径组成为(286+3×560+286)m,全长2252m,里翁-安蒂里翁大桥建成后将成为世界上最长的斜拉桥(见图1)。

They conta i ned full genes ORFs and proved to have been prolongated effectively. The result showed that the seqences of 14 genes were genes known previously and 6 genes were new .We separately chose one seqence from known and the other from unknown genes to make full-length cDNA annotation. Their code number was EST contig110 and EST contig133. We designed specific primers to amplify the two genes by RT-PCR, and then sequenced. The result was obtained as expected and proved that silico cloning genes was reliable.

对20条contigs进行了基因注释,证实它们均得到了有效延伸,具有完整开放阅读框,其中已知的猪基因序列14条,猪新基因序列6条,分别从已知猪基因序列和未知猪基因序列中各抽取一条感兴趣序列进行全长cDNA的预测,它们的编号分别为EST contig110和EST contig133,设计特异引物对两条候选序列进行克隆与测序,结果与电子克隆所得序列一致,证实了电子克隆的有效性。

The triplets are replaced by thudding full-length quavers, as the volume achieves a momentary fff.

在音量瞬间达到超强音时,三连音变成了重击的全长八分音符。

Methods. Lateral full-length radiographs of 18 normal volunteers were examined.

方法。我们对18个正常志愿者进行了椎体的外侧全长影像学检查。

According to his account, the third reclining Buddha was a 1,000 feet long and lay on the valley floor.

根据他的描述,第三座是卧佛,全长1000呎,而且被平放在河床上。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力