全长

Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

To investigate the mechanism of wood formation and lignin biosynthesis, it is important to understand the function and characteristic of OMT. In the present study, a set of primer was designed based on the conserved region of OMT genes from several woody and non-woody plants, to clone the counter part gene by PCR and RACE from Chamaecvparis formosensis Matsum., Chamaeeyparis obtusa var. Jormosana, Abies kawakarnii, Tsuga ehinensis var. Jormosana and Taiwania eryptonierioide Hayata. The cloned genes were sequenced and their amino acid sequences predicted.

我们自资料库中收集并分析已发表之草本和木本植物caffeoyl CoA3-O-methyltransferase序列，在具高保守性的序列中设计了一对引子，并对红桧、台湾扁柏、台湾冷杉、台湾铁杉和台湾杉进行OMT基因的钓取，同时配合RACE的策略，成功获得红桧、台湾扁柏、台湾杉的全长基因及台湾冷杉与台湾铁杉之部分基因片段。

Based on the sequences of CD3γ/δ, CD4L, CD8α, CD8β, we examined the distribution of CD3γ/δ, CD4L, CD8α and CD8β positive cells in carp thymus, headkidney, tunk kidney and spleen.

CD8α基因的全长cDNA序列。

Full length nucleic acid sequence of recombinant pGEM-AC-7 were determined and we found recombinant pGEM-AC-7 was 1146 bp long, inculding all open reading frame except initiation coden ATG and 192 bp 3-end non-coding reagion.

DNA序列分析和PCR鉴定表明，插入片段均由5&端特异引物单个引物扩增出来，对7号重组克隆插入片段DNA全序列分析表明，7号重组克隆插入片段全长1146 bp，包含了除启始密码子外的全部编码区和192 bp的3&端非编码区。

In this study,one pair of specific primer for mature chicken interleukin-18(ChIL-18) cDNA was designed and synthesized according to the previously published gene sequence of ChIL-18.The full length cDNA gene of ChIL-18 encoding mature active protein was amplified from LPS–stimulated MDCC-MSB1 cells by Reverse Transcription-Polymerase Chain Reaction.Then it was cloned into pMD18-T vector. Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0bp including the stop coden and the same as the published ChIL-18 cDNA sequence by Schneider K.

本研究根据已发表的ChIL-18成熟蛋白(mature ChIL-18，mChIL-18)的cDNA基因序列，设计一对特异性引物，应用反转录-聚合酶链式反应(reverse transcription-polymerase chain reaction，RT-PCR)技术从脂多糖刺激10小时活化的马立克氏病成淋巴细胞样细胞系MDCC-MSB1的cDNA中扩增出编码鸡IL-18成熟活性蛋白的全长基因，对扩增片段进行T-A克隆，经PCR、酶切鉴定及测序验证，成功获得ChIL-18成熟蛋白完整基因的克隆。

From stem to stern is thirty-six feet.

这艘快船从船头到船尾全长36英尺。

The clear water and high cliff constitute a natural landscape painting whose craftsnanship can only be created by spirits.And moreover,the 10km surface calcified landscape,according to some experts,is the only calcifies valley in the esatern china,and qualified to the application foe the world cultural relics.

水清如碧玉，山秀如诗画，有山皆奇，有水皆秀，鬼斧神工，妙境天成，是一幅仙山圣水的自然画卷，自然景观资源十分丰富，其全长10公里的地面钙化景观，被有关专家称为中国东部唯一的钙化型峡谷景观，有资格申报世界文化遗产。

It is one of the world's earliest canals.

京杭大运河的确是一项伟大的工程。它全长1774公里，是世界上最长的人工河。

The isolate BP2 and two defoliating isolates VD8 and 592 were used to inoculate the BC_1S_2 families.The resistance performance was investigated at seedling and maturity stages.

用BC_1群体构建了另一张含204个SSR位点，全长1772.54cM，标记间平均间距8.65cM海陆种间遗传图谱。

The output of the experiments demonstrated that we could only validate partial new genes of our predictions, mainly because that it was very difficult to obtain fresh human tissue samples especially those from human brain, and most of our samples were partially depredated at the time we got them

然而由于超大基因大都在脑组织中表达且拷贝数低，再加上因伦理等原因难以取得较为新鲜的人类组织样品，最终只能利用RT-PCR的方法验证了一些新基因的部分片段，证明了它们在组织中表达存在，但暂未能得到新超大基因的全长cDNA。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。