全长

Results of sequence and homology analysis show that MD 1 has 97% similarity with mark in maize chloroplast genome,a gene encoding RNA maturase involved in group Ⅱ intron splicing of RNA transcript;MD2 has 99% similarity with the gene serine/threonine phosphorylase 2C in Sporobolus stapfianus;and MD3 has 99% similarity with rice the gene encoding metacaspase,an arginine/lysine-specific cysteine protease.

序列分析和同源性比对表明，MD1与编码成熟酶的玉米叶绿体基因matK有97%的相似性，MD2与极端耐旱植物Sporobolus stapfianus编码丝氨酸/苏氨酸蛋白磷酸酶的PP2C基因有99%的相似性，MD3与属精氨酸/赖氨酸特异性半胱氨酸蛋白酶类的水稻metacaspase酶基因有99%的相似性。根据MD2片段序列，结合电子克隆和RT-PCR方法，克隆出一条1731 bp的全长cDNA序列，它编码388个氨基酸。

Objective To clone the full-length gene of staphylococcal enterotoxin A and construct its expression vector.

目的 克隆金黄色葡萄球菌肠毒素A全长基因并构建其表达载体。

Objective To clone the full-length gene of staphylococcal enterotoxin A and construct its expression vector.

导读： 目的克隆金黄色葡萄球菌肠毒素A全长基因并构建其表达载体。

We had preliminary researched on the mechanism of Stylosanthes guianensis cv. Mineirao resistant to anthracnose, a specific fragment of SG2950-1 was acquired from genome DNA of Stylosanthes guianensis cv. Mineirao by the technique of AFLP in our laboratory, we acquired both ends of the fragment of SG2950-1 by RACE from Stylosanthes guianensis cv.

我们已经对Mineirao柱花草的抗病机理进行了初步研究，并用AFLP分子标记技术从其基因组DNA中获得了一特异片断SG2950-1，利用快速扩增cDNA末端技术将基因组DNA特异片段的两端从Mineirao柱花草扩增出来，经测序后拼接得到MSRA基因的全长序列。

We have cloned a novel MnSOD gene from Tamarix androssowii , a woody plant having specific ability to live in adverse environment.

我们从抗逆性较强的木本植物紫杆柽柳中克隆到一个新的MnSOD基因，并将该全长基因在GenBank上注册，其登录号分别为AY573576和AAS77885。

Results: The anti PSA scFv/human p53 tetramerization domain fusion gene consisted of 891bp encoding 297 amino acid residues, and was the same as that reported before. The expression products of the tetrameric anti PSA scFv, which relative molecular mass was about 35 000, were confirmed by SDS PAGE and Western blot.

结果：获得了抗PSA scFv/人p53四聚功能域融合基因，基因全长891bp，可编码297个氨基酸，与已发表的抗PSA，scFv，人IgG3上游铰链区和人p53四聚功能域基因cDNA序列一致。

Full-length plasmid DNA was amplified with the above primers with a high-fidelity thermostable DNA polymerase capable of long-rang amplification.

用能扩增长片段的高保真耐热 DNA聚合酶扩增全长的质粒DNA，直接转化大肠杆菌。

Results Endogenous TIAR was primarily concentrated in the nucleus of SMCC-7721 cells.

免疫印迹证实过表达的TIAR全长及缺失体的EGFP融合蛋白分子量大小符合预期。

The subcellular localization of TIAR full length and truncated mutants were observed by using fluorescent microscopy.

酶切鉴定及测序结果显示TIAR全长及缺失体的pEGFP-C3重组表达质粒构建成功。

The efficiency of selection was monitored by comparing the number of phage recovered from the acid elution and cell lysate in each round,and by testing EGFR binding specificity of polyclonal phagescFv on CHOEGFRGFP1 and CHOK1 cell with cell ELISA. Bacterial PCR was used to select clones containing a 1 kb insert. Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。