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Results The full-length variant-specific surface antigen gene fragment from G. lamblia was found to be 2 142 bp, encoding a 713 amino acid polypeptide and contained a single open reading frame.

结果 中国人源蓝氏贾第鞭毛虫基因全长为2 142 bp,编码1条长为713 个氨基酸残基的多肽链,含有一个简单的开放阅读框。

Results The full-length variant-specific surface antigen gone fragment from G. lamblia was found to be 2 142 bp, encoding a 713 amino acid polypeptide and contained a single open reading frame.

结果 中国人源蓝氏贾第鞭毛虫基因全长为2 142 bp,编码1条长为713个氨基酸残基的多肽链,含有一个简单的开放阅读框。

Qingzang Railway is from Xi'ning to Lasa, total length is 1956 kilometers.

青藏铁路从西宁至拉萨,全长1956公里。

The results showed that total length of the leptocephali dramatically decreased during the 1st to the 4th day of experiment in the Stage II.

结果,实验第1天体长开始收缩到实验第4天的体长收缩到最短(全长范围20.60 mm ~21.69 mm)为StageⅡ的发育阶段。

Assisted by DNA2.0 and Gene2Oliga software, we optimized the codon usage and secondary structure of RNA and induced enzyme sites Cla I (237 site) and Pst I (475 site) into the gene. In the first step, fragments F1 (237 bp), F2 (238 bp) and F3 (422 bp) were separately synthesized by assembly PCR. In the second step, fragments F1, F2 and F3 were separately digested by Cla I and Pst I, and then ligated into a full length lipA gene.

首先在DNA2.0和Gene2Oliga软件辅助下对lipA基因密码子及RNA二级结构进行优化并引入Cla I(237位)和Pst I(475位)酶切位点;通过组装PCR分别合成lipA基因的各片段F1(237 bp)、F2(238 bp)和F3(422 bp);通过该基因内的Cla I和Pst I限制性酶切位点连接成完整的全长lipA基因。

There were two nucleotide mutations between F238 and Lumbricus rubellus F-III-2, which caused two amino acids mutations.

结果表明,蚓激酶基因全长为738bp,编码含7个氨基酸残基的信号肽序列和238个氨基酸残基的成熟肽序列。

Two fragments from Malvastrum coromandelianum samples were cloned and sequenced.

对赛葵样本Fz1 DNA-A进行了克隆和序列测定,Fz1 DNA-A全长2741个核苷酸。

Filamentous primordial gonad formed gradually in 15-30 mm-TL juveniles (40-60 DAH), which was located in the posterior end of the abdominal cavity just beneath the mesonephric ducts closing to the body wall.

原始性腺在全长15~30mm(孵化40~60d)的幼鱼中逐渐发育完善,呈细线状,位于腹腔后部中肾管下方紧贴体壁。

Objective To obtain the full-length cDNA sequences of CYP2E1, CYP2D5, ECHS1, which may be related with non-alcoholic fatty liver disease, from Microtus fortis.

目的 克隆CYP2E1、CYP2D5、ECHS1三个与非酒精性脂肪肝可能相关基因的cDNA全长基因,为进一步研究非酒精性脂肪肝的发病机制提供依据。

They all contain 13 protein coding gene,2 rRNA gene,22 tRNA gene and 1 D-Loop.The base composition for the four nucleotides is A-32.0%,C-27.6%,G-14.7%,T-25.8%,And it is A-32.5%,C-26.9%,G-14.1%,T-26.5%for Yellow-throated Marten.But there are some definite differences in base composition,the using of Initiation codon and Stop codon,and the mode of repeat sequences in control region.The codon usages of Manes have bias,and the ttiird locations of codon of protein-coding genes have the higher frequency in using A and C.There may be some relativity with the content of A and C in D-loop,namely,it has relation with the mode of repeat sequences.The complete mitochondrial genome of the Sable and Yellow-throated Marten were submitted to GenBank,and the accession number are FJ429093 and FJ719367 respectively.3、The complete mitochondrial genome of 6 other species of Mustelidae from GenBank and some sequences of D-loop from the 6 species were aligned.

分析紫貂大兴安岭亚种、长白山亚种、阿尔泰亚种和北欧亚种间的基因流及进化历史得知:大兴安岭种群与新疆种群和长白山种群间的基因流水平最高(Nm=0.1260和0.1427),新疆与长白山种群间最低Nm=0.0053紫貂种群在进化过程中可能发生过种群膨胀,经历过种群增长过程。2、对紫貂和黄喉貂的线粒体全基因组结构进行分析发现:全长分别为16 523bp和16549bp,均包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个非编码序列区(D-Loop区,紫貂全序列中碱基组成为A-32.0%,C-27.6%,G-14.7%,T-25.8%,黄喉貂为A-32.5%,C-26.9%,G-14.1%,T-26.5%;基因排列顺序与日本貂和貂熊的一致,但碱基组成、起始密码子和终止密码子的使用及控制区中串联重复序列模式等均存在一定差异。

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