全长

A new full-length cDNA clone was isolated from the fiber cDNA library of Gossypium hirsutum L.

利用PCR筛选方法从陆地棉纤维cDNA文库中筛选到一个全长cDNA序列，命名为GhbZIP。

The phylogenetic analysis of Gymnothorax was reported in this paper by being based on DNA sequences of mitochondrial Cytb gene.17 species of Cytb gene full-length sequences were obtained and their length of Cytb gene was 1140bp.All sequences were analysised by using biological software, the phylogenetic tree was constructed by using three kinds of common contribution phylogenetic tree methods and the phylogeny of 17 species of Gymnothorax was deduced.

本论文采用线粒体Cytb基因为分子标记，共获得裸胸鳝属鱼类17种Cytb基因序列全长为1140bp的部分序列，应用分子生物学软件对结果进行分析，使用3种常见的建树方法：NJ法、ME法和MP法重建系统发育树，对裸胸鳝属17种鱼类的系统关系进行研究，为探讨裸胸鳝属鱼类的分子进化，确定它们之间的关系提供分子生物学方面的证据。

The key research progresses and results that we accessed by the project were as follows: 1 A pair of degenerated primers were designed in the conserved domain which based on the amino acid sequence of HAK in Arabidopsis thaliana. The 500 bp fragment of HAK were amplified from the roots of Puccinellia tenuiflora by RT-PCR. The full sequence of HAK were obtained by 5' and 3' RACE methods and were determined and analyzed.

本项目主要取得了以下研究进展和成果：（1）根据已有HAK转运蛋白氨基酸序列在保守区域设计简并PCR引物，用反转录PCR方法，扩增出500bp的小花碱茅根HAK片段，通过5''和3''RACE的方法获取了该HAK的全长序列，完成了测序和序列同源性分析。

Capitata L., were dispersed along the chromosome lengths, with a predominant distribution in the pericentromeric or proximal regions and some heterochromatic arms.

大基因组的玉米和大麦的所有染色体都被密集地标记，并在染色体全长显示出强标记区与弱标记或不标记区的交替排列。

From using the same cervida for the Hydropotes intermis mitochondrial gene and its ratio of cytb,Detected 140 bp Variable sites,Insertion and deletion were not found.By comparison.

本文测定了麋鹿线粒体cytb基因全序列，并对麋鹿线粒体cytb基因全长1140bp进行了分析，利用同为偶蹄类鹿科的獐子线粒体cytb基因与其比对，检测到了140个变异位点，未发现插入和缺失。

A micronuclear version of the GARP gene was amplified by polymerase chain reaction. The macronuclear DNA molecule carrying the GARP gene is 460 bp long and shows the characteristics of macronuclear chromosomes of hypotrichous ciliates.

大核中该基因的染色体全长460 bp，基因两端具有下毛类纤毛虫大核特有的端粒序列(C4A4C4A4C4A4C4)，开放读框内含有一个TGA(88-99)密码子，在游仆虫中编码为半胱氨酸。

Objective To establish an effective technique in reservation and function reconstruction of a leg, in which all the muscles except gastrocnemius necrosed induced by the delayed necrosis of all the intermuscular vessels, the foot was linked only by skin, fascia and tibia, Methods The leg was linked with foot only by the skin, fascia, caput mediale of gastrocnemius and tibia when all the muscles, intermuscular vessels and fibula were removed.

目的 探索迟发性小腿全长肌间血管全部坏死而导致除腓肠肌内侧头以外小腿肌肉全部坏死，仅以皮肤、筋膜、胫骨连足的情况下肢体保留和功能重建的方法。

The results of cDNA sequencing examined that the sBD-1 cDNA contain a open reading frame of 192 bases which encoded a 64 amino acid prepro-peptide and the prepro-peptide contained the β–defensin consensus sequence of six invariantly spaced cysteine residues.

我们采用 cDNA 末端快速扩增即 RACE 技术，主要包括锚式 PCR RACE 技术和反向嵌套 PCR RACE 技术分别扩增出蒙古绵羊 sBD-1 的 3ˊ末端和 5ˊ末端，经测序得到 sBD-1 的 cDNA 全长序列。

Of the 2,693 NCGR-cDNAs that have no homologies in KOME cDNAs, 1,751 matched to japonica genomic sequences, and were expressed in both varietyes, while their expression varies between two subspecies.

2，693条与粳稻日本晴的全长cDNA无同源匹配cDNA中，1，751条NCGR-cDNA与日本晴的基因组序列有同源匹配。

Total RNA was extracted from Alopex lagopus spleen using Trizol reagent. A full length cDNA library was constructed using SMARTTM cDNA library Construction Kit.

用Trizol试剂提取蓝狐脾脏总RNA，用SMART cDNA文库构建试剂盒构建全长cDNA文库。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。