全长

Sequence analysis and phylogenetical study showed that this cDNA contained 778 nucleotides, with a 627 bp open reading frame flanked with a 44 bp 5' UTR and a 107 bp 3' UTR. The encoded 208 amino acids housefly Attacin shared a high similarity of 50%~70% with that of the other dipterous insects. In addition, the phylogenetical analysis also indicated that the Attacin from housefly was in the same branch with those of other species, suggesting that they come from the same ancestor.

家蝇Attacin的cDNA全长778 bp，其中包括627 bp的开放式读码框，44 bp的5ˊ末端非编码区(5ˊUTR)和107 bp的3ˊ末端非编码区(3ˊUTR)，相应的蛋白产物含208个氨基酸；与其他双翅目昆虫的Attacin具有很高的相似性，约50%～70%；以邻接法(Neighbor-Joining，NJ)所构建的系统关系表明家蝇的Attacin与其他双翅目昆虫的Attacin起源于共同的祖先。

Field-tests show: grouting bolt exists the question of effective anchorage length; shear stress distribution is disuniform and concentrated in the front 1/3 of the fixed anchoring part.

在现场对普通拉力型锚杆进行了试验，试验表明注浆锚杆存在有效锚固长度问题，沿锚固段全长锚杆与浆体间剪应力分布极不均匀，主要集中在锚固段的前1／3。

Land gross area kilometre of ninety-nine thousand six hundred square, peninsula coasting makes an appointment with 17000 kilometre endlong include islands of great capacity

国土总面积9.96万平方千米，半岛海岸线全长约17000千米包括岛屿海

Standard of ornamental design acceptance of work: Ornamental design project is finishing dry hind, just can check and accept; Check and accept ornamental design project, whether does the breed that should check ornamental design, norms, design accord with design requirement; Ornamental design should be installed firm, its quality asks to reach tolerance, should accord with: The level of form ornamental design and perpendicular tolerance, every meters must not be more than 5 millimeter, must not be more than 3 millimeter endlong; The tolerance of position of alone ornamental design, must not be more than 10 millimeter; Ying Guangjie of ornamental design surface, design clarity, juncture rigor, must not interstitial, warp, edge damage drops the blemish such as horn; Back up spends exterior Ying Guangjie, design clarity, without break.

花饰工程验收标准：花饰工程完工并干燥后，方可验收；验收花饰工程，应检查花饰的品种、规格、图案是否符合设计要求；花饰应安装牢固，其质量要求及允许偏差，应符合：条形花饰的水平和垂直允许偏差，每米不得大于5毫米，全长不得大于3毫米；单独花饰位置的允许偏差，不得大于10毫米；花饰表面应光洁，图案清晰，接缝严密，不得有裂缝、翘曲、缺棱掉角等缺陷；裱花表面应光洁，图案清晰、无裂缝。

In order to develop antibacterial peptide biologics for prevention and control of endometritis of dairy cow,a pair of primers was designed and synthesized according to the sequence of bovine antibacterial peptide gene published in GenBank.The total RNA was purified from the dairy cow endometrial cells with the Total RNA Isolation Kit.

为研发防治乳牛子宫内膜炎的抗菌肽生物制剂，根据已发表的牛抗菌肽基因序列设计了1对引物，用试剂盒提取乳牛新鲜子宫内膜总RNA，经RT-PCR扩增，将克隆的抗菌肽BNBD5基因全长编码区的cDNA连接到原核表达载体pET30a中，经IPTG诱导原核表达，通过Western-blot检测融合蛋白BNBD5的表达水平。

The identification was made by means of restriction enzym eanalysis, PCR and DNA sequencing. Results: The murine entire coding cDNA of 2508bp was enamplified from spleen cells. There was 100% homogenous nucleotide sequence as compared with standard sequence (NM021297) of TLR4 from Genbank.

结果：扩增得到的小鼠TLR4基因cDNA全长2508bp，编码536个氨基酸，Blast结果分析表明，该cDNA与基因库中发表的序列(NM021297)具有100%的同源性。

Holland is the country with the most complete bicycle transportation system, with 22,000 kilometers of bike lanes, equalling ten times the length of its freeway. Eighty percent of Dutch citizens own more than one bicycle. In addition, bicycle sharing schemes are available in cities like Paris, London, Barcelona and Copenhagen. These schemes allow residents to use public bicycles at a low price or even for free.

荷兰是脚踏车交通系统最完善的国家，脚踏车车道全长22，000公里，是高速公路的10倍；高达八成的荷兰人，拥有一辆以上的脚踏车；此外，公用脚踏车计画也在许多城市施行，包括巴黎，伦敦，巴塞隆纳和哥本哈根，民众可以低价甚至免费去使用公共脚踏车。

Combining SSH and cDNA microarray to screen of up-regulated ESTs in the water stress Erianthus arundinaceus based on the analysis of up-regulated ESTs,a full-length cDNA sequence was cloned from sugarcane through Rapid Amplification of cDNA Ends method,and the gene expression character was analyzed by real time RT-PCR.

应用消减文库技术结合cDNA芯片技术筛选水分胁迫诱导的基因的EST序列，根据筛选到感兴趣的上调表达基因的EST序列，用RACE技术获得SSADH的全长cDNA序列，并通过实时荧光RT-PCR技术对该基因的表达特征进行分析。

Moreover, abundant expression of full-length forms of BCL11A is developmentally restricted to adult erythroid cells.

此外，全长型BCL11A的大量表达限制成人红系细胞发育。

A gene sequence of the anthropogenic adiponectin spherical fragment obtained by the invention absolutely accords with a known gene sequence, and compared with the full-length adiponectin, the hydrolyzed anthropogenic adiponectin spherical fragment has stronger biological activity, fast reaction after dosage, good bioavailability and better clinic application prospect, especially when the diabetes patient receives a recanalization treatment after a myocardial infarction, the anemically myocardial damage of the diabetes patients can be eased by the exogenetic dosage.

本发明公开了一种人源性脂联素球状片段用于治疗糖尿病缺血性心脏病药物的应用，本发明所得到的脂联素球状片段的基因序列与已知的基因序列完全相符，与全长的脂联素相比，其水解以后的脂联素球状片段有着更强的生物学活性，而且给药后反应迅速，生物利用度好，有着更好的临床应用前景，特别是对糖尿病患者在发生心肌梗死后接受血管再通治疗时，通过外源性给药，以减轻糖尿病患者缺血心肌损伤。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。