全长

An infectious clone of the sat-RNA of BBSV-X was also constructed by placing the cDNA under the control of the 35S promoter. This plasmid could infect Chenopodium amaranticolor with the help of in vitro transcripts of BBSV-X.

构建了35S启动子控制下的BBSV卫星RNA全长侵染性克隆，侵染性检测表明，所获得的3个克隆均有较好的侵染性。

Salina by RT-PCR and 3￠ Rapid Amplification of cDNA Ends, which shared homology with Chlamydomonas (48%), Arabidopsis thaliana (50%), Homo sapiens (46%), etc.

序列分析显示克隆的cDNA全长1 650 bp，具有一定保守性，与衣藻、拟南芥和人的相似性分别为48％、50％和46％。

ESTs of chickpea (Cicer arietinum L.) were downloaded from the database of NCBI and some redundant or low quality sequences were removed. Finally, 11224 non-redundant ESTs with total length of 6198092bp were obtained. SSRIT (Simple sequence repeat identification tool), a web tool for SSR hunting, was used to analyze the sequences.

从NCBI公共数据库下载获得27904条鹰嘴豆表达序列标签EST，去除其中低质量的和冗余序列后得到全长为6198092bp的11224条无冗余EST。

Methods: Five 10-23 deoxyribozymes (DZ1-DZ5) with 2 phosphorothioate groups at 5' and 3' end were designed and synthesized. Full sequence ET-1 RNA was transcripted and FAM-labeled 10-23 deoxyribozyme was used to select cleavable 10-23 deoxyribozyme in vitro and to detect intracellular uptake. The content of ET-1 mRNA was measured by semi-quantitative RT-PCR after 10-23 deoxyribozyme was transfected into cultured neonatal rat cardiomyocytes.

体外转录ET-1全长RNA底物，设计并合成5条ET-1 10-23脱氧核酶(DZ1~DZ5)，其5'及3'端各有2个核苷酸硫代修饰，体外切割ET-1RNA底物，筛选有效脱氧核酶；5'标记荧光素FAM的10-23脱氧核酶瞬间转染新生大鼠心肌细胞以观察对10-23脱氧核酶的摄取；采用半定量RT-PCR检测ET-1基因的表达。

And, we selected some representative species of Collembola and representative groups of Protura, Diplura, Insecta and Arustacean to sequence and analyze their 18S rRNA completely gene sequences, 28S rRNA D6-D7 domain and nearly completely gene sequences to make the studies about the phylogeny relationships of the main families and orders of Collembola, discuss the questions such as the reliability about the new four orders system to Collembola, the monophyletic and ordinal phylogenetic relationships of them and the situation of the special groups, the phylogenetic relationships about Collembola with other groups and the evolution of Hexapod.

同时选取弹尾纲的代表种类和原尾纲、双尾纲、昆虫纲、甲壳动物等节肢动物部分类群，进行了18S rRNA基因全序列和28S rRNA基因D6-D7区以及近全长序列的测定、分析，对弹尾纲重要科属的亲缘关系、进化地位进行分子系统学研究，讨论弹尾纲最新分类系统中四目的确定性、各目单系性、四目的系统关系、特殊类群的分类地位，以及弹尾纲和其它类群之间亲缘关系、六足动物进化等问题。

Hutiao Corge Situated between the Haba Snow Mountains in the southwest of Shangri-la County and the Yulong Snow Mountains in the northwest of Lijiang, Hutiao Corge, 20 kilometers long and 213 meters high and with 18 dangerous shoals, is a gorge celebrated worldwide. It is divided into three sections: the upper Hutiao, the middle Hutiao and the lower Hutiao.

虎跳峡 是世界著名的大峡谷，位于香格里拉县东南部的哈巴雪山与丽江西北部的玉龙雪山之间，海拔落差3900多米，全长16公里，迂回线长约20公里，落差213米，分上虎跳、中虎跳、下虎跳三段，共18处险滩，江面最窄处仅30余米。

Sequencing of this 4.3kb DNA fragment revealed that there is a β-glucosidase gene urnbgl3A with an ORF of 1956bp, encoding product shared 63% identity and 79% similarity with a beta-glucosidase (GenBank NO:AAX16378.1) of uncultured murine large bowel bacterium BAC 31B, and shared 50% identity and 67% similarity with a beta-glucosidase-related glycosidases (GenBank NO:ZP-00308419.1) of Cytophaga hutchinsonii.

测序结果表明在4.3kb的片段上有一个全长为1956bp的ORF，编码一个可能的β-葡萄糖苷酶基因umbgl3A，其编码产物与一个来源於小鼠大肠未培养细菌的β-葡萄糖苷酶(GenBank Acession NO：AAX16378.1)一致性为63%、相似性为79%；与哈氏噬纤维菌的β-葡萄糖苷酶(GenBank Acession NO：ZP-00308419)的一致性为50%、相似性为67%。

The cloning of Actin lay a the theoretical foun- dation for the further expression and biological analysis of Actin full-length cDNA sequence.

土耳其斯坦东毕吸虫肌动蛋白的全长cDNA的克隆为进一步表达及其生物学性能的分析提供了理论基础。

And 1993 . In this thesis, we, for the first time, successfully isolated the full-length cDNA for the ECP 63, an embryogenic cell protein, from carrot (Daucus carota L.).

为实验材料，首次成功地分离了ECP63 cDNA全长，并以此cDNA为探针对其在体细胞胚胎发生和合子胚胎发生过程中的基因表达进行了深入的研究。

Results The whole genome of CVB4 jlu06 strain consisted of 7 395 nucleotides encoding 2183 amino acids, which showed high homologies to other CVB4 strains, with the mutations of 10 amino acids. Phylogenetic analysis proved that CVB4 jlu06 strain showed close relationship to HumanCB4 and POLG_CXB4 strains while showed remote relationship to CXB2 strain. Unlike other non-diabetogenic strains, CVB4 jlu06 strain showed common amino acids with diabetogenic strains at 4 sites in 3A region of non-structural protein.

结果 CVB4 jlu06株基因组全长7395个核苷酸，编码2183个氨基酸，与其他CVB4株的同源性较高，且发生了10个氨基酸的变异，与HumanCB4和POLG_CXB4亲缘关系较近，与CXB2亲缘关系较远；CVB4 jlu06株与具有潜在致糖尿病性病毒株，在非结构蛋白3A区4个位点有共同的氨基酸，与非致病毒株不同。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。