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This method is more simple and inexpensive than screening cDNA library, and can be easily adapted to clone other genes. An Amorpha fruticosa cDNA clone encoding UDP-glucose pyrophosphorylase, a key enzyme producing UDP-glucose in the synthesis of sucrose and cellulose, was cloned by using this method.

本论文利用此方法首次从紫穗槐中克隆了与纤维素合成前体----尿苷二磷酸葡萄糖的代谢有关的尿苷二磷酸葡萄糖焦磷酸化酶基因和与木质素合成相关的4-香豆酸:CoA连接酶(4CL)基因的全长cDNA序列,并由此推测出相应的氨基酸序列。

Methods: We retrospectively compared the measurements of lower limb alignment that were obtained with use of supine intraoperative fluoroscopy with those that were obtained with use of a full-length standing anteroposterior radiograph of the lower extremity.

研究方法:本研究回顾性的对两种方法(站立位全长前后位片和仰卧位透视片)所得的下肢对线排列资料进行对比。

In the present study, full-length rDNA sequences from the domestic and wild type of Chinese oak silkworm, Antheraea pernyi (GenBank No. GU073314 and GU073315, respectively) were obtained and comparatively analyzed on their genetic variation.

测定了放养型和野生型柞蚕的rDNA ITS-2全长序列(GenBank登录号:GU073314、GU073315),并分析了二者之间的遗传差异。

Atrickle[1]of trucks flows along its single-lane carriageway, which stretches for less than 1km.

在这条全长不到1公里的单车道上,仅稀稀拉拉地行驶着几辆卡车。几个小时里都没有一列火车通过。

Using PCR and RACE technique, We obtain the cDNA and genomic DNA sequence of the lac1 gene from Auricularia polytrica.

本文利用PCR和RACE技术首次从毛木耳AP4菌株中获得编码漆酶基因的cDNA及其基因组全长序列,基因组大小为2514 bp。

The keys to operation and therapeutic effects were summarized and analyzed, Results 22 cases received follow-up surveys. 6 months to 2 years of follow-up surveys showed that all the children had the balanus and penis body exposed completely.

结果 本组25例中22例术后获随访观察6月~2年,所有患者阴茎头及阴茎全长显露满意,矫正效果良好。

The mouse embryonic stem cells cDNA library was screened by yeast two-hybrid assay,some candidate molecules were get,including DDX39 DEAD (Asp-Glu-Ala-Asp box polypeptide 39, BAT1A(HLA-B-associated transcript 1A), NAC1 ( nucleus accumbens-1 ), BicD2 (bicaudal D homolog 2)、MLL3 (myeloid/lymphoid or mixed-lineage leukemia 3). NAC1 and MLL3 were associated with regulation of gene transcription. DDX39 andBAT1A were associated with pre-mRNA processing and mRNA export from nucleus to cytoplasm. BicD2 was associated with the regulation of Golgi body.

通过利用酵母双杂交方法,以小鼠全长Plk1作为诱饵蛋白筛选小鼠胚胎干细胞cDNA文库中与其相互作用的蛋白,获得了DDX39DEAD(Asp-Glu-Ala-Aspbox polypeptide 39、BAT1A(HLA-B-associated transcript 1A)、NAC1(nucleus accumbens-1)、BicD2(bicaudal D homolog 2)、MLL3(myeloid/lymphoid or mixed-lineage leukemia 3)等几个候选蛋白,其中NAC1、MLL3与基因的转录调控有关,DDX39、BAT1A与mRNA前体的剪切、加工及mRNA从细胞核转运到细胞质紧密相关,而BicD2与高尔基体的调控紧密相关,提示我们Plk1与基因转录、mRNA前体的剪切、加工、mRNA的运输及胞质分裂中高尔基体的调控有关。

Crystal shape of B-Hm-16 was bipyramidal, but in B-Pr-88 bipyramidal, spherical and irregular shapes were observed.

另一个基因cry9Ba是通过PCR特异扩增获得的,其编码区全长3354bps,编码1117个氨基酸,蛋白分子量为126.4kDa,cry9Ba基因正在申请注册。

It is join Erie and an aqueduct that install rude lake, the river is winding and labyrinthian, the cloth way that state of American new York has south is much, north installs the Yang Ge of rude province to press down to Canada, full-length only 54 kilometers, altitude falls continuously from 174 meters however reach 75 meters, bizygomatic breadth of upriver reach river 3 kilometre of 2 ~, surface fall only 15 meters, current also relatively delay.

是连接伊利湖和安大略湖的一条水道,河流蜿蜒而曲折,南起美国纽约州的布法多,北至加拿大安大略省的杨格镇,全长仅54公里,海拔却从174米直降至75米,上游河段河面宽2~3千米,水面落差仅15米,水流也较缓。

Ma Bian Zhou Dolphin Berth of CSPC Nanhai Petrochemicals Project is an oil terminal with a capacity of 150,000t. The length of the berth is 412m. The berth consists of one working platform, one auxiliary platform, 2 breasting dolphins, 4 mooring dolphins and a 48-meter-long service berth.

南海石化马鞭洲港区码头工程为一座15万吨级油轮码头,全长412m,由一个工作平台、一个辅助平台、2个靠船墩、4个系缆墩组成和一个48m长的辅助船舶泊位组成。

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