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The full-length cDNAs of rice and wheat S-adenosylmethionine decarboxylase gene were isolated and characterized. The rice SAMDC cDNA is 1560 bp in length, encoding a polypeptide of 398 amino acids, and the wheat SAMDC cDNA is 1351 bp in length, encoding a polypeptide of 392 amino acids. Both the rice and wheat SAMDC proteins contain the conserved proenzyme cleavage site and the PEST domains, which are commonly found in the SAMDC protein sequences of all eukaryotes.

克隆了水稻和小麦SAMDC基因的全长cDNA序列,所获得的水稻SAMDC基因序列长1560 bp,编码一个由398个氨基酸组成的多肽,小麦SAMDC基因序列长1351 bp,编码一个由392个氨基酸组成的多肽,二者均含有真核生物SAMDC蛋白所拥有的两个保守的氨基酸结构域:酶原剪切位点和PEST结构域。

Sequence analysis showed that the full-length cDNA sequence of Sc-SAMDC (GenBank Accession number: GQ246459) was 1 968 bp, with three open reading frames, tiny ORF, upstream ORF and main ORF. The mORF was 1200 bp encoding 399 amino acid residues with a molecular mass of 43.6 kD, and the deduced protein had two highly conserved function domains (proenzyme cleavage site and PEST domain).

序列分析显示,甘蔗Sc-SAMDC基因(GenBank Accession number: GQ246459) cDNA全长1968 bp,存在3个读码框(袖珍读码框tORF、上游读码框uORF和主读码框mORF),mORF长1200 bp,编码399个氨基酸的SAMDC酶原,预测分子量为43.6 kD,该酶原含有两个高度保守的功能结构域(酶原剪切位点和PEST结构域)。

The PCR product was cloned into pCR BluntⅡ-TOPO vector to form subclone and then sequenced.The subclone product were cloned into the improved pBluescript Ⅱ KS vector in proper order by suitable enzyme sites to obtain viral full length genomic cDNA clone.

根据测序结果,选择合适的酶切位点将各个亚克隆产物逐段克隆入改造过的pBluescript Ⅱ KS载体中,从而获得了病毒的全长基因组cDNA克隆。

The 6 AChE Genes from L.bostrychophila,L.entomophila and L.decolor were demarcated into Insect TypeⅠAChE Gene and Insect TypeⅡAChE Gene.4 Gene cloning of nAChR and mRNA expression level in L.bostrychophilaTwo nicotinic acetylcholine receptor subunit genes,Lb al and Lb a8,were cloned from the psocid L.bostrychophila.The full length cDNAs of Lb a1(GenBank Accession No.: EU871527) and Lb a8GenBank Accession No.

昆虫的两类AChE严格的区分,其中Ⅰ型AChE先与脊椎动物AChE聚合后才与Ⅱ型AChE相聚,说明两类AChE基因在物种分化前就已经分化。4嗜卷书虱烟碱型乙酰胆碱受体基因克隆及其mRNA表达水平研究利用RT-PCR和RACE技术成功克隆获得了嗜卷书虱2个烟碱型乙酰胆碱受体亚基的全长序列,分别命名为Lb a1(GenBank登录号:EU871527)和Lb a8(GenBank登录号:EU871526)。

Analysis of the characteristics of such damage to surrounding rock, the use of grouting and bolting control of surrounding rock stability and to strengthen the roof support strength, take full advantage of their load-bearing capacity of rock supporting the principle of a reasonable research grouting, anchoring rod supporting technology, including high water and quick-setting grouting reinforcement material between two groups, a total length of the roof using resin anchor bolts and high strength prestressed anchor to strengthen the small aperture and the two to help support longer anchoring resin bolting.

分析该类巷道围岩破坏特点,提出运用注浆及锚杆支护控制巷道围岩稳定、加强顶板支护强度、充分利用围岩自身承载能力的支护原理,研究了合理的注浆、锚杆支护技术,包括高水速凝材料注浆加固两帮、顶板采用树脂全长锚固高强度锚杆和小孔径预应力锚索加强支护及两帮树脂加长锚固锚杆支护。

Analysis of the characteristics of soft rock roadway, combined with practical application, designed to spray a new anchor shell supporting structure and proposed mechanism of interaction with the rock that anchors the shell surrounding rock and shotcrete support system as an entity, anchor-body system and the deep rock formation deep rock control system, housing system and the formation of shallow shallow rock rock control system, through the bolt will be deep and shallow rock together, sprayed on the rock-body system to play the role of three-dimensional constraints, bolting technology, including high water, quick-setting grouting material between two groups, roof anchoring resin full-length high-strength bolts and small-diameter prestressed anchor to strengthen support for the conservation and help the two longer anchoring resin bolting.

分析软岩巷道的特点,结合实际应用,设计了锚壳喷新型支护结构,并提出了与围岩相互作用机理,认为围岩与锚壳喷支护体系作为统一体,锚体系统与深部围岩形成深部围岩控制系统,壳体系统与浅部围岩形成浅部围岩控制系统,通过锚杆将深、浅部围岩共同作用,喷体系统对围岩起到三维约束作用,锚杆支护技术,包括高水速凝材料注浆加固两帮、顶板采用树脂全长锚固高强度锚杆和小孔径预应力锚索加强支护及两帮树脂加长锚固锚杆支护。

To construct an infectious full-length cDNA clone of Asia1/JS/China/2005 strain with Arg-Gly-Asp receptor recognition site.

构建含有RGD受体结合位点口蹄疫病毒Asia1/JS/China/2005株的全长感染性cDNA克隆。

Edge communications companies seamless steel pipe production line length of 400 meters, 100 meters wide by more than 300 sets of ancillary equipment, of which there is tube grinding, sawing equipment, tube furnace, piercing machine, rolling machine, stepper heating furnace, micro-stretch reducing mill, cooling bed, straightening machine, pipe cutting machine, testing machines, finishing equipment.

力通联公司无缝钢管生产线全长400米,宽100米,由300多套附属设备组成,其中有管坯修磨、锯切设备、管坯加热炉、穿孔机、轧管机、步进式加热炉、微张力减径机、冷床、矫直机、切管机、探伤机、精整设备等。

7 Meters length, weighed about 1 ton, the longest of 10 meters, is the largest reptile.

全长6~7米,重约1吨,最长达10米,是现存最大的爬行动物。

Methods:Fifty paired embalmed cadaveric humeri (twenty-five pairs:fourteen from male donors and eleven from female donors) were scanned in medial-lateral and anterior-posterior position according to the humeral retroversion by CT. Images of the humeri in the transverse planes at the lowest border of neck,20mm and 40mm distal of LBN(LBN-20、LBN-40), isthmus, head-neck anterior-posteriorwere obtained. Sixty-one extracortical and intracortical parameters were measured exactly by image analytic computer software that included offset, head position, head-shaft angle, head to tuberosity height, head thickness, curvature radius, articular surface arc, neck diameter, isthmus position, proximal and distal border of isthmus, maximum coronal and sagittal diameter of medullary canal and thickness of cortical bone in four planes, including LBN, LBN-20,LBN-40 and isthmus.

50根成对防腐肱骨(男14对,女11对)按肱骨头扭转角置于冠状位和矢状位,行肱骨全长,头颈矢状面,解剖颈下缘及其下20mm、40mm,髓腔狭窄部四平面CT扫描,由CT软件测量冠、矢状位髓腔内外参数共61项,包括头心—干轴距,头位置,头干角,头—结节高度差,头厚度,头半径,关节面张角,解剖颈直径,髓腔狭窄部位置,解剖颈下缘及其下20mm、40mm和狭窄部四个平面髓腔的最大冠、矢状径,皮质骨厚度等。

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主要做了如下几个方面的工作:对伸臂位于顶部的单层框架—筒体模型进行分析,提出了等效转动约束的概念和转动约束刚度的表达式。

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