全长

Objective: To clone the complete gene of nucleoprotein of H3N2 subtype avian influenza virus, and to express recombinant NP in E.coli for the function research.

目的：获得H3N2亚型禽流感病毒核蛋白全长基因，并在大肠杆菌中表达，以用于对NP功能的研究。

Five pieces of ESTs showed strong homology to it, and they were assembled to one 772bp cDNA sequence by DNASTAR software.

结果显示ECRG-4近似cDNA全长序列为772bp，其中含有一447bp的完整阅读框，编码148个氨基酸。

MMCP-10 full-length cDNA includes an open reading frame of 942 base pairs.

MMCP-10全长cDNA包含一个942个碱基的开放的阅读框架。

Enlighten the coasting that do obeisance to is full-length 72 kilometers, and palmy island has odd Zhumeila the coasting with 78.6 long kilometers.

迪拜的海岸线全长72公里，而单朱美拉棕榈岛就有78.6公里长的海岸线。

The expression of CD59 on the cell membrane was tested by cell immunohistochemistry and flow cytometry. Results pALTER plasmid containing CD59 was cut with restriction enzymes and a 496 bp fragment obtained by electrophoresis, which was complete conformity with the insert.

将两种含有不同突变体的人CD59全长cDNA序列重组pALTER质粒，应用阳离子脂质体导入法与pcDNA共转染CHO细胞，用G418筛选阳性克隆，应用细胞免疫组化及流式细胞仪检测CD59在CHO细胞表面的表达。

Methods: Two recombinant palter plasmid containing whole CDNA sequences of HMCD59 transfected CHO cells by lipofectamine with PCDNA respectively. Then G418 was used to select positive clone.

分别构建两种含有HMCD59全长CDNA序列的重组pALTER质粒，并与pCDNA3质粒，按3：1比例混合后，运用脂质体介导法共转染CHO细胞(编号为HM5-CHO及HM6-CHO)，用新霉素类似物G418初步筛选出阳性克隆。

A river of the Peloponnesus in southern Greece flowing about113 km(70 mi) to the Ionian Sea.

阿尔斐俄斯河希腊南部伯罗奔尼撒的一条河，全长约113公里（70英里），流入爱奥尼亚海

"Dreamed up by Socialist Mayor bertrand Delanoe,"Paris Plage" stretches over three kilometres of closed-off road on Paris's Right bank from the Louvre art gallery to the elegant Ile Saint-Louis."

"社会党人巴黎市长贝特朗·德拉诺市长构想的""巴黎海滩""位于巴黎右岸隔离的马路上，一直从卢浮宫延伸到华丽的圣路易岛，全长超过三千米。"

In this study, the full-length hsp70 cDNA was firstly isolated and sequenced from planarian Dugesia japonica (Djhsp70) using rapid amplification of cDNA end technology.

研究采用 RACE (Rapid amplification of cDNA end)技术首次从日本三角涡虫中克隆出hsp70 (Djhsp70)全长cDNA序列。

This does not, rice revenge me, my face is now full, and you have leftovers after the pock grown more than I am.....

这不，米饭报复我了，现在全长我脸上了，你们剩饭以后麻子长得比我还多。。。。。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。