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Methods RT-PCR and RACE methods were applied to clone the full-length panallergen gene from Caryota mitis pollen. The sequence of the insert was analyzed.

利用RT-PCR结合RACE技术克隆短穗鱼尾葵花粉中泛变应原profilin的全长基因,并进行序列分析。

The ORF of profilin of Caryota mitis pollen was amplified by RTPCR using specific primers and cloned into the expression vector pET-28a.

结果克隆获得了短穗鱼尾葵花粉profilin的全长基因,由608个碱基组成,开放阅读框为396个碱基,编码131个氨基酸。

IgE reactivity of recombinant Caryota mitis pollen profilin was investigated by western blot. Results The Caryota mitis pollen profilin gene was cloned. The cloned fragment was 608bp in length, with an 396bp open reading frame coding for 131 amino acids.

结果 克隆获得了短穗鱼尾葵花粉profilin的全长基因,由608个碱基组成,开放阅读框为396个碱基,编码131个氨基酸。

The Chinese Muntjac's mitochondrial genome, consisting of 16354 base pairs which encode genes for 13 proteins, 2 rRNAs, and 22 tRNAs, is similar to those mammals in both order and orientation.The sequence of rRNA gene ,some of the protein-coding regions and tRNAs are highly homologous in mammals. Differences existing in the length and sequence of the D-loop regions account for the variations in mammals mitochondrial genomes.

与其他哺乳动物线粒体基因组全序列的比较研究发现:全长为16 354bp的小麂线粒体基因组同样编码13种蛋白质、2种rRNA和22种tRNA,除了用于调控线粒体DNA复制和转录的D-Loop区以外,小麂线粒体基因组各基因长度、位置与其他哺乳动物相似,其编码蛋白质区域和rRNA基因与其他哺乳动物具有很高的同源性。

At water temperature of 16.0-17.2 ℃, the process of embryonic development could be divided into 24 stages, which were described as follow: fertilized egg at 0 min, 2-cell stage at 75 min post fertilization; myotome forming stage at 43 h PF, and hatching-out stage at 94 h PF when the accumulative temperature reached 1616.8 ℃.h, and the average size of larvae was about 3.8 mm in body length.

梭鲈胚胎发育过程可分为24期,在水温16.0~17.2℃范围内,受精75 min开始第1次卵裂,受精后43 h开始形成器官,受精后约94 h开始出膜;刚出膜的仔鱼全长平均为3.8 mm;从受精到孵化出膜总积温为1 616.8℃。h。

It is 16 meter long and speeds at 120 nautical miles with the capacity of carrying 12 passengers.

这架直升机全长16米,巡航速度每小时120海里,可搭乘12名乘客。

After every 60μl of 30% HRP were injected into abdominal section of the esophagus of 20 rats respectively,the labelled neurons were found bilaterally within nodose ganglions of vagus nerve,dorsal root ganglia(T9~L3),thoracic sympathetic ganglions and coeliac ganglion .

将30%辣根过氧化酶溶液注入大鼠食管腹段近贲门处外膜下,切片用四甲基联苯胺法成色,结果如下:标记细胞主要分布于双侧结状神经节,双侧的脊神经节(主要在T9~L3)、双侧的胸交感节(T9~L1)和腹腔节,在延髓的标记细胞见于疑核全长内,主要分布于疑核咀侧端。

The author investigates the effect of mean stress and stress's amplitude with the non symmetry low cycle fatigue experiment of two kinds of PD3 rail steel (hot rolling and off line heat treatment) produced by PanZhiHua steel company.

通过对攀钢生产的两种PD3钢轨钢(热轧和离线全长热处理)的非对称低周载荷疲劳实验,比较了平均应力和荷载幅值对两种PD3钢轨钢寿命的影响。

Only one candidate gene is in the region, and the full-length ORF of SKC1 was obtained from the cDNA library of Nona by using PCR.

该区域内只有一个候选基因,利用PCR技术从Nona的cDNA文库中获得SKC1的全长ORF。

Changhong Road Food Street with Jiqingmen Street in its southmost and Shuiximen Street in northmost, is 1.2 kilometers long and 40 meters wide commercial food street, integrating culture, catering and water relaxation.

长虹路美食街南起集庆门大街,北至水西门大街,全长1.2公里,路幅宽40米,是一条集文化、餐饮、亲水休闲于一体的美食商业街。

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The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作:对伸臂位于顶部的单层框架—筒体模型进行分析,提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。

Its advantage is that it can be used in smaller units.

其优点在于可以在较小的单位中应用。