全无的

And yet this time removed was summer's time, The teeming autumn big with rich increase, Bearing the wanton burden of the prime, Like widowed wombs after their lords' decease: Yet this abundant issue seemed to me But hope of orphans, and unfathered fruit, For summer and his pleasures wait on thee, And thou away, the very birds are mute.

可是别离的时期恰好是夏日；和膨胀着累累的丰收的秋天，满载着青春的淫荡结下的果实，好像怀胎的新寡妇，大腹便便：但是这累累的丰收，在我看来，只能成无父孤儿和乖异的果；因夏天和它的欢娱把你款待，你不在，连小鸟也停止了唱歌；或者，即使它们唱，声调那么沉，树叶全变灰了，生怕冬天降临。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

Corolla purple to blue or white or yellow to red, zygomorphic, inside glabrous or puberulent; tube campanulate-cylindric to broadly tubular, sometimes ampliate basally, throat sometimes constricted, 1-4 X longer than limb, 2-8(-12) mm in diam.; limb slightly 2-lipped to 2-lipped; adaxial lip 2-lobed, slightly shorter than to nearly as long as abaxial lip; abaxial lip 3-lobed to 3-sect, lobes equal or subequal, apex rounded to acute.

花冠紫色到蓝色或白色或黄色对红色，左右对称，被微柔毛的里面无毛或；圆筒状的钟状的筒部对宽管状，有时基部，有时缢缩的喉，X长于瓣片，2-8毫米直径；对二唇形有点二唇形的瓣片； 2裂的正面唇，稍短于到近等长于背轴唇；背面的唇3浅裂的到3全裂，裂片等长或近等长，先端圆形的到锐尖。

Methods:the clinic data of 172 cases performed by lc were analyzed retropectovely to disscuss the operation skill and the owoiding of complications.results:there are 3 cases with bile leakage and 5 case with hemorrhage during operation.3 cases were converted to open operations midway with a rate of 1.74%.there are no such severe complications as biliary ducts injury,postoperational hemorrage and bile leakage.conclusions:be familiar with the anatomic variation of cholecystic vessels and biliary ducts,reasonable operation skill,standard manipulation and abdominal operation in time can decrease the complications of lc.

回顾性分析我院172例lc患者的临床资料，就手术技巧与并发症预防进行探讨。结果：术中发现胆漏3例；术中出血5例；中转开腹3例，中转开腹率为1.74％。全组无胆管损伤、术后出血及胆漏等严重并发症。结论：熟悉胆囊血管和胆管的解剖变异，合理的手术技巧、规范的操作、及时果断的中转开腹可以最大限度的减少lc并发症的发生。

Part 2. Measurement of macular light sensitivity and FM 100-hue test in normal prese- nium and geratic people. The macular light sensitivity 、macular foveal light sensitivi- ty in 64 (82 eyes) and hue discrimination in 75 (100 eyes) normal presenium and geratic people were examined with macular threshold (white、red、blue and green color light) program of the Humphreh Field Analyzer and FM 100-hue test respectivity. The re- sults showed that the light sensitivity in four color light threshold was all declined with age. The mean error score square of each spectral region and whole spectral was increased with age, the loss of light sensitivity and hue discrmination was a linear correlated with age. The light sensitivity of the different field was compared, except blue color light threshold, the light sensitivity was decreased with the increasing of the eccentricity, white and red color light threshold in the inferior field showed lower sensitivity than that in the superior field.

二、老年前期及老年期正常人的黄斑阈值及FM100-色调试验检测采用Humphrey视野分析仪黄斑阈值程序和FM100-色调试验仪分别对82例（82眼）和75例（100眼）老年前期及老年期正常人进行了黄斑光敏度、黄斑中心凹光敏度及色调分辨力的测定，结果显示四种色光阈值的光敏度均随年龄增加而降低，FM100-色调试验各个色区及全色区错误记分方根随年龄增加而增高，光敏度及色调分辨力的降低与年龄呈线性相关；不同视野的光敏度比较，除蓝色光阈值外，光敏度均随视野偏心度的增加而减少，视野的上半部光敏度较下半部低；视野的鼻侧与颞侧及各象限的光敏度无显著差异。

The rules of exchange is complied with the Contract Law of P.R.of China and GB/T1876-2003(Norms of Bulk Commodity Electronic Trading) which is promulgated by Ministry of Commerce.Thanks to the E-commerce technology that we can organize the domestic and abroad mineral deals by quoting,matchmaking,ordering on our website(www.bbgme.com).Following the transaction principles of good faith,openness,fairness and impartiality,we carry out three trading models:spot transaction,bidder auction transaction an mid-to-long term spot transaction.Meanwhile, we will hold the minning machinery and equipment fairs,exploration right and mining rights transfer business.

中心根据《中华人民共和国合同法》、《大宗商品电子交易规范》等相关法律法规，运用电子商务信息技术组织国内外的矿产品交易商、依托本中心网站www.bbgme.com的交易平台，通过互联网直接上网报价、配对，以网上订货的方式交易模式，同时开设矿山设备展销和矿业权转让业务，提供信息、资金、仓储、质检、物流、结算、融资等一体化的全方位服务，是国内首家实现全电子化及无场地交易的矿产品交易中心，致力于为客户提供健全、透明和高效的交易平台，帮助客户实现集资和投资的目标。

Basal leaves 5--10; petiole 2--6 cm; blade ternate, or sometimes simple, pentagonal or broadly ovate in outline, 0.6--1.5 × 1--2.4 cm, thinly papery, subglabrous or abaxially puberulent; leaflets petiolulate, rhombic, 2- or 3-lobed or 2- or 3-partite, sometimes 1--2 × 3-sect and with ultimate segments lanceolate-linear; central lobe rhombic-obovate, 3-lobed or 3-partite.

基生叶5-10；叶柄2-6厘米；叶片三出，或者有时单，五边形的或宽卵形轮廓的， 0.6-1.5 * 1-2.4 厘米，薄纸质，近无毛或背面被微柔毛；小叶具小叶柄，菱形的，2或3裂或2或3 分裂的，有时1-2×3全裂并且具末回裂片的有时披针形线形的；中央裂片菱形倒卵形，3浅裂或分裂。

The human cartilages are composed of chondrocyte and extracellular matrix,the form of chondrocytes are hypertrophy and the quantity are less;the ECM of cartilage are compised of type II collagen and proteoglycan. Articular cartilages are all hyaline with little fibers. Trauma and arthritis are the main cause of cartilage injury,the ommilayer injury ofcartilage can be recovered by marrow,but because of without stimulation mechanism,the new tissues are merely fibrocartilages,they can not be coincide with hyaline cartilage in menchanics;the purely damage of articular cartilage can not stimulate chondrocyte to regenerate because of without blood circulation,thus,the plerosis of articular catilage can not depend on the proliferation of local chondrocyte.Ever since,people tried their best to find a way to reconstruct articular cartilage.

造成人体关节软骨损伤的原因主要为创伤和关节炎，关节软骨全层损伤可由于骨髓中间充质干细胞的高速增殖修复，但这种修复由于缺乏相应的刺激机制，只能形成纤维软骨，而不能形成符合关节生理、力学要求的透明软骨；单纯软骨部分损伤软骨组织内无血管，软骨细胞迁移迟缓，无法使损伤区域软骨细胞再生，因此，关节炎及关节创伤后的软骨修复不能依赖于软骨细胞的增殖和迁移。

The human cartilages are composed of chondrocyte and extracellular matrix , the form of chondrocytes are hypertrophy and the quantity are less; the ECM of cartilage are compised of type Ⅱ collagen and proteoglycan. Articular cartilages are all hyaline with little fibers. Trauma and arthritis are the main cause of cartilage injury, the ommilayer injury ofcartilage can be recovered by marrow, but because of without stimulation mechanism, the new tissues are merely fibrocartilages, they can not be coincide with hyaline cartilage in menchanics; the purely damage of articular cartilage can not stimulate chondrocyte to regenerate because of without blood circulation, thus, the plerosis of articular catilage can not depend on the proliferation of local chondrocyte.

造成人体关节软骨损伤的原因主要为创伤和关节炎，关节软骨全层损伤可由于骨髓中间充质干细胞的高速增殖修复，但这种修复由于缺乏相应的刺激机制，只能形成纤维软骨，而不能形成符合关节生理、力学要求的透明软骨；单纯软骨部分损伤软骨组织内无血管，软骨细胞迁移迟缓，无法使损伤区域软骨细胞再生，因此，关节炎及关节创伤后的软骨修复不能依赖于软骨细胞的增殖和迁移。

The human cartilages are composed of chondrocyte and extracellular matrix , the form of chondrocytes are hypertrophy and the quantity are less; the ECM of cartilage are compised of type Ⅱ collagen and proteoglycan. Articular cartilages are all hyaline with little fibers. Trauma and arthritis are the main cause of cartilage injury, the ommilayer injury ofcartilage can be recovered by marrow, but because of without stimulation mechanism, the new tissues are merely fibrocartilages, they can not be coincide with hyaline cartilage in menchanics; the purely damage of articular cartilage can not stimulate chondrocyte to regenerate because of without blood circulation, thus, the plerosis of articular catilage can not depend on the proliferation of local chondrocyte. Ever since, people tried their best to find a way to reconstruct articular cartilage.

中文题名人骨髓基质干细胞成软骨诱导及多孔复合材料作为细胞载体的体外实验研究副题名外文题名 Cartilage induction of human mesenchymal stem cells and experiment on compound porous materials as cells' scaffold in vitro 论文作者刘晓岚导师周江南学科专业外科学研究领域\研究方向学位级别博士学位授予单位中南大学学位授予日期2003 论文页码总数68页关键词骨组织工程软骨细胞骨髓基质干细胞壳聚糖高分子外消旋聚乳酸馆藏号BSLW /2003 /R68 /10 造成人体关节软骨损伤的原因主要为创伤和关节炎，关节软骨全层损伤可由于骨髓中间充质干细胞的高速增殖修复，但这种修复由于缺乏相应的刺激机制，只能形成纤维软骨，而不能形成符合关节生理、力学要求的透明软骨；单纯软骨部分损伤软骨组织内无血管，软骨细胞迁移迟缓，无法使损伤区域软骨细胞再生，因此，关节炎及关节创伤后的软骨修复不能依赖于软骨细胞的增殖和迁移。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新