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On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

我们根据在脲或盐酸胍存在下的底物反应动力学方程求得游离酶和酶底物二元、三元复合物的微观动力学常数,结果表明:①酶-底物二元、三元复合物的失活速度明显慢于游离酶,说明两个底物二氢叶酸和NADPH对酶的失活都具有一定程度的保护作用;②在脲作用下,酶的失活和构象变化均为单指数项过程,而且酶的活力丧失和三级结构变化是同时发生的,说明二氢叶酸还原酶的脲变性可能是一个"全或无"的两态过程;③在盐酸胍作用下,酶的构象变化为两相过程,而失活则是单指数项过程,酶分子构象变化的快相速度常数与失活速度常数基本一致,因此我们认为二氢叶酸还原酶的盐酸胍变性过程中存在一个没有活力、但仍具备一定空间结构的变性中间体。

Some paper, the Pall Mall Gazette I think, describing the dress-rehearsal of one of my plays, spoke of you as following me about like my shadow: the memory of our friendship is the shadow that walks with me here: that seems never to leave me: that wakes me up at night to tell me the same story over and over till its wearisome iteration makes all sleep abandon me till dawn[34a]: at dawn it begins again: it follows me into the prison-yard and makes me talk to myself as I tramp round: each detail that accompanied each dreadful moment I am forced to recall: there is nothing that happened in those ill-starred years that I cannot recreate in that chamber of the brain which is set apart for grief or for despair: every strained note of your voice, every twitch and gesture of your nervous hands, every bitter word, every poisonous phrase[34b] comes back to me: I remember the street or river down which we passed, the wall or woodland that surrounded us, at what figure on the dial stood the hands of the clock, which way went the wings of the wind, the shape and colour of the moon.

有份报纸,我想是《泼尔穆尔报》吧,报道了我一出戏的彩排,说你像影子似的跟随着我:对你我友谊的回忆,就是在这里随我左右的影子,像是永不分离似的——深夜里唤我醒来,一遍又一遍地说着同一个故事,直磨得人睡意全无,醒到天明[34a];天明时分又开始了,跟着我到牢房外的院子里,害得我一边步履沉重地走着一边喃喃自语——我被迫回想着每一个痛苦时刻的每一点细节,在那些个倒霉的年头里发生的事,没有哪一件我不能在那留给悲伤和绝望的脑室里再造重演:你每一点不自然的话音,每一个紧张兮兮的手势,每一句冷言恶语[34b],都涌上了心头;我记着我们到过的街道和河流,四周的墙壁和树林,时钟的针正指着哪一点,风正吹向哪一面,月色月影又是什么模样。

The items of respiration and circulation recorded before and 10, 20, 30, 40min after pneumoperitoneum as well as 5 min after deflation abdomen were as follows: blood pressure, heart rate, sphygmus oxygen saturation (SpO_2),...

结果术毕清醒时间硬膜外组明显短于全麻组(P·05),气腹后20min、30min潮气量硬膜外组明显小于全麻组(P·05),各时点呼吸频率、分钟通气量、气道压硬膜外组分别明显快于、大于、低于全麻组(P·05),其余各项指标两组无显著差异。结论在妇科腹腔镜手术中,两种麻醉方式对呼吸、循环的影响无显著差异。

We cannot, if we would, play the part of China, and be content to rot by inches in ignoble ease within our borders, taking no interest in what goes on beyond them, sunk in a scrambling commercialism; heedless of the higher life, the life of aspiration, of toil and risk, busying ourselves only with the wants of our bodies for the day, until suddenly we should find, beyond a shadow of question, what China has already found, that in this world the nation that has trained itself to a career of unwarlike and isolated ease is bound, in the end, to go down before other nations which have not lost the manly and adventurous qualities.

虽然我们并不能,但若是我们在自己的国家扮演中国的那种在骄奢淫逸中一寸一寸的腐烂的社会角色,除了对自己,周围发生的一切则丝毫不感兴趣,沉浸各种乱七八糟的利益之中,不追求更高层次的生活,全无勤劳和冒险的渴望,每天只奔忙于来自身体的需求,我们会突然间发现,在疑团之外中国所已经找到的结局——在这个世界中,当一个国家把自身训练成并不好战并且把隔绝和安逸当作国界的时候,最终将倒在另一个富有勇敢的冒险精神的国家面前。

The Stairs were such as whereon Jacob saw [ 510 ] Angels ascending and descending, bands Of Guardians bright, when he from Esau fled To Padan-Aram in the field of Luz, Dreaming by night under the open Skie, And waking cri'd, This is the Gate of Heav'n [ 515 ] Each Stair mysteriously was meant, nor stood There alwayes, but drawn up to Heav'n somtimes Viewless, and underneath a bright Sea flow'd Of Jasper, or of liquid Pearle, whereon Who after came from Earth, sayling arriv'd, [ 520 ] Wafted by Angels, or flew o're the Lake Rapt in a Chariot drawn by fiery Steeds.

那阶梯,像是雅各离开以扫,逃往巴旦亚兰去的途中,在路斯的野地里露夜时梦见的,一队队灿烂的天使卫士上去下来,醒后叫道:"这是天门。"阶梯的每一级都蕴含着神奇的寓意,也不永久固定在那儿,时常被拉到天上去,踪影全无。下面是一片似从碧玉或珍珠的溶液流漾而成的,晶莹的海,后来从地球上来的,必经此海,要么由天使驾帆,要么乘火马拉的轻车飞越水面而来这里。

The mayor of New Yorkduring the September 11th 2001 attacks, he led in national polls for most of2007. But he bizarrely chose to sit out the early primary states where hisrivals were insurmountably strong.

这位在2001年&9·11&恐怖袭击时在任的前纽约市长在2007年的绝大多数时间里的全国民意调查中稳拔头筹,但他却在先前举行预选的州中全无胜绩,而他的竞争对手在这些地区取得了不可超越的成功。

Sessile spikelet longer than rachis internode; lower glume elliptic-ovate to oblong, papery to leathery, 5–9-veined, marginally 2-keeled, keels pectinately spiny, often winged at apex; upper glume 3-veined, keeled along midvein, otherwise almost flat, often narrowly winged on lower keel; lower floret staminate, palea present; upper floret bisexual, upper lemma entire, awnless.

无梗小穗长于轴节间;下部颖片椭圆状卵形到长圆形,纸质到革质,5-9脉,具刺的2龙骨状,龙骨状的pectinately,通常具翅的在先端的稍微; 3脉的上面颖片,龙骨状沿中脉,几乎平的否则,龙骨状的通常狭翅在下部;雄蕊花的下部的小花,内稃宿存;全缘的上面小花两性,上面外稃,。

As share price of concept of new energy resources advance triumphantly all the way, more and more enthusiasm are more radical, bold swarm into, lifted tide of surfy play chess of rich of new energy resources in the stock market, with in former days already the Internet bubble photograph of broken up is compared, is the difference that tide of new energy resources fries subject matter nominally because of heat merely a bit devoider bubble?

随着新能源概念股价的一路高歌猛进,越来越多的热情更加激进、大胆的涌入,并在股市中掀起了波涛汹涌的新能源博弈浪潮,与昔日早已支离破碎的互联网泡沫相比,难道新能源浪潮仅仅因热炒题材名称上的差异全无一点泡沫?

Nor are mine cars with thy tongue's tune delighted, Nor tender feeling to base touches prone, Nor taste, nor smell, desire to be invited To any sensual feast with thee alone: But my five wits, nor my five senses can Dissuade one foolish heart from serving thee, Who leaves unswayed the likeness of a man, Thy proud heart's slave and vassal wretch to be: Only my plague thus far I count my gain, That she that makes me sin, awards me pain.

我耳朵也不觉得你嗓音好听,就是我那容易受刺激的触觉,或味觉,或嗅觉都不见得高兴参加你身上任何官能的盛酌。可是无论我五种机智或五官都不能劝阻痴心去把你侍奉,我昂藏的丈夫仪表它再不管,只甘愿作你傲慢的心的仆从。不过我的灾难也非全无好处:她引诱我犯罪,也教会我受苦。

In Xinjiang, the Talimu River has shrunk by a third causing complete water depletion in the lower reaches. Bones of wild animals who had depended on the "dessert source of life" littered the erstwhile lush river banks. In the Great Northern Wilderness in Heilongjiang, Siberian tigers, bears, river deer, red-crowned cranes, swans and sturgeons disappeared. Similar situation also happened in Xishuangbanna, China's biggest rainforest. Asiatic elephants were driven to the neighboring Burma, Laos and Thailand due to extensive land reclamation.

在新疆,塔里木河缩短了三分之一,导致下游水量的全面损耗,曾依靠&沙漠水源&生存的野生动物纷纷死亡,尸体散落在曾经郁郁葱葱的班克斯河边,在黑龙江的北大荒,西伯利亚虎、熊、驯鹿,丹顶鹤,天鹅和鲟鱼踪影全无;相似的情况也发生在西双版纳,中国最大的雨林,巨大的垦荒范围将亚洲象驱赶到邻国缅甸,老挝和泰国。

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For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降,而后引起了信用卡大规模的亏损。

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老秋先生为中国秋阳翻译集团的董事长和孔子商学院的院长,致力于国学的研究和推广。