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aim to assess relationship between the increased lfa-1expression in intestine graft and acute rejection,and inhibitory effects of fk506on the expression of lfa-1.methods heterotopic small intestine transplantation was performed on inbred sd and wistar/a rats.the rats were randomly divided into4groups:control groups;isograft group(wistar/a→wistar/a);allograft group(sd→wistar/a);allograft plus fk506group(sd→wistar/a+fk506),

目的 探讨大鼠移植小肠组织内淋巴细胞相关抗原-1(lfa-1)的异常表达与急性排斥反应之间的关系,及fk506对其的抑制作用。方法选用近交系封闭群sd和wistar/a大鼠进行全小肠异位移植。实验分4组:非手术对照组;同基因移植组(wistar/a→wistar/a);异基因移植组(sd→wistar/a);异基因移植加用fk506治疗组[sd→wistar/a+fk506

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果:1、哇巴因作用于晶状体钠泵后,光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙,电镜下全层细胞空泡化、线粒体肿胀出现髓样结构,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后,光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离,电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后,光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整,电镜下细胞器丰富、细胞功能旺盛,RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后,光镜下晶状体质膜完整、细胞排列紧密规整,电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝,RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后,光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化,电镜下囊侧膜内有高电子密度带、细胞核仁丰富,RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后,光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏,电镜下质膜变性、线粒体肿胀,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

The compounds were identified by physical and spectroscopic techniques.Result:Six compounds were isolated and identified as 4,4′-biisofraxidin(1),esculetin(2),fraxetin(3),scoparone(4),isofraxidin(5),scopoletin(6),respectively.Conclusion:Compound 1 is a novel natural product.

结果:首次从草珊瑚干燥全草中分离得到6种具有抗菌活性的香豆精类化学成分,分别为4,4′-双异嗪皮啶(4,4′-biisofraxidin,1),秦皮乙素(esculetin,2),秦皮素(fraxetin,3),滨蒿内酯(scoparone,4),异嗪皮啶(isofraxidin,5),东莨菪内酯(scopoletin,6)。

Theoretical studies on various geometrical structures of Hypophosphorous acid were carried out using DFT theoretical methods,at the B3LYP/6—311++G** level.Intramolecular proton transfer isomerization has also been studied using the intrinsic reaction coordinate.

采用量子化学密度泛函B3LYP方法,在6—311++G**基组水平上全优化得到了硫代次磷酸的硫酮式和硫醇式两种构型异构体以及相应的对映异构体,同时对分子内质子转移异构化反应微观机理进行了理论分析。

Theoretical studies on various geometrical structures of Hypophosphorous acid were carried out using DFT theoretical methods, at the B3LYP/6-311 ++ G(superscript **) level. Intramolecular proton transfer isomerization has also been studied using the intrinsic reaction coordinate.

采用量子化学密度泛函B3LYP方法,在6-311++G基组水平上全优化得到了硫代次磷酸的硫酮式和硫醇式两种构型异构体以及相应的对映异构体,同时对分子内质子转移异构化反应微观机理进行了理论分析。

The optimum reaction conditions of the synthesis of isoamyl acetate with Nafion NR50 resin membrane as catalyst were studied.

以全氟磺酸树脂膜为催化剂得到冰乙酸和异戊醇反应合成乙酸异戊酣适宜的反应条件。

Objective:To investigate the effect of total intravenous anesthesia with propofol on splanchnic perfusion in patients undergoing laparoscopic cholecystectomy.

目的:探讨异丙酚全凭静脉麻醉对腹腔镜下胆囊切除术患者内脏灌注的影响。方法:30例择期行LC患者,随机分为异氟醚静吸复合麻醉组和异丙酚全凭静脉麻醉组。

And yet this time removed was summer's time,The teeming autumn, big with rich increase,Bearing the wanton burden of the prime,Like widow'd wombs after their lords' decease:Yet this abundant issue seem'd to meBut hope of orphans and unfather'd fruit;For summer and his pleasures wait on thee,And, thou away, the very birds are mute; Or, if they sing,'tis with so dull a cheer That leaves look pale, dreading the winter's near.

可是别离的时期恰好是夏日;和膨胀着累累的丰收的秋天,满载着青春的淫荡结下的果实,好像怀胎的新寡妇,大腹便便:但是这累累的丰收,在我看来,只能成无父孤儿和乖异的果;因夏天和它的欢娱把你款待,你不在,连小鸟也停止了唱歌;或者,即使它们唱,声调那么沉,树叶全变灰了,生怕冬天降临。

The results showed that the spatial distribution range of soil fertility in Qinling forest area on south slope was soil pH volue 5.97~6.89,organic matter 6.6~50.1 g·kg~-1, total N 0.5~3.8 g·kg~-1 ,available N 33.6~257.3 mg·kg~-1,available P 0.81~5.8 mg·kg~-1,available K 38.9~262.4 mg·kg~-1,clay particle content 11.3%~18.1%,capacity of exchangeable base 68.5~310.2 mmol·kg~-1 respectively; The spatial variation degree of available P, organic matter, and total N was big,whereas clay particle content and active acid pH value was small;the spatial variation degree of soil pH value, organic matter, available P, clay particle content, and capacity of exchangeable base decreased with the deep of soil,whereas soil total N、available N, and available K increased.

结果表明,秦岭南坡土壤肥力指标在整个土壤剖面空间分异范围分别为:土壤pH值5.97~6.89,有机质含量6.6~50.1 g·kg-1,土壤全N含量0.5~3.8 g·kg-1,碱解N含量33.6~257.3 mg·kg-1,速效P含量0.81~5.8 mg·kg-1,速效K含量38.9~262.4 mg·kg-1,粘粒含量11.3%~18.1%,阳离子代换量68.5~310.2 mmol·kg-1;其空间分异程度以速效P、有机质和土壤全N含量较大,土壤粘粒含量和土壤pH值较小;随着土层的加深,土壤pH值、有机质、速效P、土壤粘粒含量和阳离子代换量空间分异程度减小,而土壤全N、碱解N和速效K含量空间分异程度增大。

An all-optical adder/subtractor unit with the help of terahertz optical asymmetric demultiplexer is proposed.

提出一种基于太赫非对称光解复用器的全光加减法单元,该单元使用一组全光全加法器和光学&异或门&。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

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A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。