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When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2－4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L－1 after their number counted.

选取2～5代的小鼠胚胎成纤维细胞，待其至对数生长期倒掉或吸掉培养基，加入含10 mg/L丝裂霉素C的细胞全培养液5 mL，置37 ℃，体积分数0.05的CO2饱和湿度温箱中培养二三小时，在6孔板中加入0.1%明胶包被，放置30 min以上，倒掉或吸掉含丝裂霉素C的培养基，磷酸盐缓冲液清洗5遍，尽量除去丝裂霉素C，加入2 mL 0.05%的胰蛋白酶消化细胞，镜下观察，当细胞间出现裂隙，细胞变圆时（约2～4 min），立即加入等量的全培养液终止消化，并用吸管反复吹打，使之成为单细胞悬液，在细胞计数板中央放置专用的盖玻片，用玻璃虹吸管吸取细胞，让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液，至盖玻片下被液体充满为止。

A shot-gun DNA sequence strategy was employed,in which the mitochondrial genome library of Muntiacus reevesi has been constructed to obtain the complete mitochondrial genome sequence.

通过建立麂属动物小麂线粒体DNA文库、鸟枪法测序，获得了小麂线粒体基因组全序列并对其基因组成、蛋白质的编码序列、tRNA基因等结构作了详细分析，这也是国内有关哺乳动物线粒体基因组全序列的首次报道。

They all contain 13 protein coding gene,2 rRNA gene,22 tRNA gene and 1 D-Loop.The base composition for the four nucleotides is A-32.0%,C-27.6%,G-14.7%,T-25.8%,And it is A-32.5%,C-26.9%,G-14.1%,T-26.5%for Yellow-throated Marten.But there are some definite differences in base composition,the using of Initiation codon and Stop codon,and the mode of repeat sequences in control region.The codon usages of Manes have bias,and the ttiird locations of codon of protein-coding genes have the higher frequency in using A and C.There may be some relativity with the content of A and C in D-loop,namely,it has relation with the mode of repeat sequences.The complete mitochondrial genome of the Sable and Yellow-throated Marten were submitted to GenBank,and the accession number are FJ429093 and FJ719367 respectively.3、The complete mitochondrial genome of 6 other species of Mustelidae from GenBank and some sequences of D-loop from the 6 species were aligned.

分析紫貂大兴安岭亚种、长白山亚种、阿尔泰亚种和北欧亚种间的基因流及进化历史得知：大兴安岭种群与新疆种群和长白山种群间的基因流水平最高(Nm=0.1260和0.1427)，新疆与长白山种群间最低Nm=0.0053紫貂种群在进化过程中可能发生过种群膨胀，经历过种群增长过程。2、对紫貂和黄喉貂的线粒体全基因组结构进行分析发现：全长分别为16 523bp和16549bp，均包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个非编码序列区(D-Loop区，紫貂全序列中碱基组成为A-32.0%，C-27.6%，G-14.7%，T-25.8%，黄喉貂为A-32.5%，C-26.9%，G-14.1%，T-26.5%；基因排列顺序与日本貂和貂熊的一致，但碱基组成、起始密码子和终止密码子的使用及控制区中串联重复序列模式等均存在一定差异。

Myriophyllum aquaticum Verdcourt is a commonly cultivated, and nearly naturalized, species in Taiwan, characterized as follows: plants dioecious (only female plants known in China); aerial leaves glaucous or light bluish green; all leaves whorled, never entire, pinnately divided with linear segments; bracteoles subulate with 1(or 2) lateral lobes.

Myriophyllum aquaticumVerdcourt一通常栽培的，植株雌雄异株的(只女性的植株被在中国知道)；气生叶苍白的或浅蓝绿色；全部叶轮生，从未全缘的，成羽状全裂具线形裂片；小苞片钻形，具(1或)2侧叶裂片。

The pioneering environmentalist, technology thinker, and founder of the Whole Earth Catalog has written a new manifesto, Whole Earth Discipline , in which he defends genetic engineering, nuclear power, and other longtime nemeses of the green left as good for the planet.

环境保护论者先驱、技术思想家、以及《地球目录全编》创始人已经写下了新的宣言《地球全科》，他在书中为遗传工程、核能、以及其他就绿色左翼而言为时甚久的人力所不能克服或做不到的事情辩解为对这颗行星有益。

Basal leaves 2-6, long petiolate; stipules over 1/2 adnate to petioles, broadly lanceolate, membranous, margin sparsely denticulate and ciliate or entire, apex acuminate; petioles 3-9 cm at anthesis, usually green, sometimes purplish, glabrous, nitid, elongated at fruiting to more than 20 cm; leaf blade glabrous on both surfaces or pubescent along veins, 3-sect, segments shortly stipitate, lateral segments deeply 2-fid, central segment deeply 2- or 3-fid, terminal lobes highly variable in shape and size, ovate-lanceolate, lanceolate, oblong, or linear-lanceolate, margin irregularly dentate or shallowly divided, sometimes triparted to pinnatiparted or 3-lobed, apex obtuse or acute.

基生叶2-6，具长叶柄；托叶超过1/2的贴生至叶柄，宽披针形，膜，边缘疏生细齿和具缘毛或全缘，先端渐尖；叶柄3-9厘米在花期，通常绿色，有时略带紫色，无毛，有光泽的，在果期拉长到超过20厘米；叶片两面无毛沿脉具短柔毛，3全裂，裂片具短柄，侧裂片2深裂，中裂片2或3深裂，顶裂片形状和大小极易变，卵状披针形，披针形，长圆形，或线状披针形，边缘不规则牙齿或浅裂，有时3深裂到羽状深裂或3浅裂，先端钝或锐尖。

The results indicate that the high efficiency and stable performance of shortcut nitrogen removal in MBR-PBBR process is attributed to the predominance of functional microbiology Nitrosomonas eutropha during the period of operation,which occupies over 54%of total bacteria.However,large amount of organic carbon can destroy the predominance of N.eutropha,whose proportion decreased to 31%at TOC/N=0.3.The diversity of microbial community in MBR increases along with operating time.

结果发现，生物强化的MBR-PBBR工艺能实现相对稳定和高效的捷径生物脱氮，主要归功于MBR中的功能微生物Nitrosomonas eutropha在运行过程中始终保持优势地位，占全菌数的比例大于54%；然而，当外加有机碳源较高(TOC/N≥0.2)时，可动摇氨氧化菌的优势地位，N.europha占全菌数的比例在TOC/N=0.3时下降至31%。

The proliferation stimulating effects on whole and nonadherent splenocytes of mice were assayed by MTT method; The cell cycles of whole splenocytes were tested with flow cytomytry.

通过MTT法测定小鼠全脾细胞或不粘附细胞的增殖活性，流式细胞仪检测小鼠全脾细胞的细胞周期。

Benzene perfluorous nonene ether was prepared by the reaction of perfluo-rous nonene (C9F18) and phenol,and the molecular structure of which was identified by IR and NMR spectra.

以全氟壬烯、苯酚和氯磺酸为主要原料，经醚化、磺化和中和反应制备对全氟壬烯氧基苯磺酸表面活性剂，用红外光谱和19FNMR，1HNMR对分子结构进行了表征。

Two kinds of basic structure modes, thoroughly transparent structure and nontransparent structure, are analyzed respectively, and the reliability model is proposed for the former structure.

分析了机载公共设备的两种基本控制结构：全透明结构和非透明结构，同时分析了机载公共设备综合管理系统全透明结构的任务可靠性模型。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。