兔耳

With the basal stem of short spike L.brevitaba and its coterminous stolon, clonal ramet taken from grasslands which were non-degraded, light-degraded, moderate-degraded and heavy-degraded as the materials, the differences of stolon number and its length and the dry weight per unit of length of short spike L.brevitaba stolon from different degraded grassland were studied.

方法］以未退化、轻度退化、中度退化、重度退化草地上挖取的短穗兔耳草基茎及与其相连的匍匐茎和无性系分株为材料，研究不同退化草地上短穗兔耳草匍匐茎的数量、长度和单位长度匍匐茎干重的差异。

Results In control group,three weeks after the epithelization of the wound,the thickness of the hypertrophic tissue was 3-4 times of ventro ear skin. Under microscope,the dermis was hyperplasia and got thicker,consisted with amount of fibroblast cells,collagen and vessels,the collagen was untidy,nodule or vortex,and the cartilage could be observed in some region.

结果：对照组兔耳创面上皮化后3周时，增生厚度为兔耳腹侧皮肤全层厚度的3～4倍，镜下可见真皮组织明显增生、变厚，由大量成纤维细胞、胶原组织及血管组成，胶原排列不整齐，呈结节或旋涡状分布，部分可见对应处软骨细胞增生。

Retinal photocoagulation was induced in the left eyes after mydriasis with tropicamid under general anesthesia, then 1% iodine sodium was injected in the vein of the rabbits at a dose of 40 mg/kg at the same time; their right eyes were not treated.

选用健康成年青紫蓝兔10只，美多丽散瞳后在全麻状态下行左眼眼底激光光凝，同时按40 mg/kg的剂量自兔耳缘静脉注入1%过碘酸钠溶液，考虑动物的生存质量，模型动物右眼均未予处理。

Bacterial pyrogen was injected into denervated rabbbitear and novocain blocked rabbit knee joint capsule to see whether pyrogen induces fever through nervous reflex mechanism via local receptors or not.

为观察细菌致热原是否通过局部感受器——神经反射机制引起发热而进行下列实验：把细菌致热原注入去神经兔耳和完整神经兔耳皮下；把细菌致热原注入事先用奴弗卡因封闭的兔膝关节囊和事先注射生理盐液的兔膝关节囊；分别比较两组动物的发热效应。

Methods Three different wound healing models were established: removing skin, perichondrium and cartilage, removing skin and perichondrium, removing skin. The process of wound healing was observed morphologically and histologically.

建立三种兔耳皮肤创伤愈合模型，A组在切除兔耳皮肤的同时，切除皮下软骨及软骨膜；B组切除皮肤及软骨膜但保留软骨；C组只切除皮肤，保留软骨及软骨膜。

Methods Rabbits auricular cartilage without perichondrium was cut into 1.5 mm×1.5 mm nieces, which were smeared on the three dimensional polylactic acid foams, then implanted into subcutaneous pocket. The implants were taken out at 3rd and 6th month respectively, thespecimens were used for gross inspection and histological analysis.

将兔耳软骨切割成大小为1.5 mm×1.5 mm的耳软骨片，均匀地涂布在具有三维空间结构的聚乳酸泡沫支架上，然后将此复合物埋植于兔背皮下，分别于移植后3、6个月取出标本，行大体观察及显微镜下检查。

Methods rabbit's auricular cartilage without perichondrium was cut into 1.5 mm×1.5 mm nieces, which were smeared on the three dimensional polylactic acid foams, then implanted into subcutaneous pocket. The implants were taken out at 3rd and6th month respectively, thespecimens were used for gross inspection and histological analysis.

将兔耳软骨切割成大小为1.5 mm×1.5 mm的耳软骨片，均匀地涂布在具有三维空间结构的聚乳酸泡沫支架上，然后将此复合物埋植于兔背皮下，分别于移植后3、6个月取出标本，行大体观察及显微镜下检查。

Methods Autologous BMMSC of New Zealand rabbits were acquired and induced into chondrogenic differentiation by adding transforming growth factor-β1 (TGF-β1), dexamethasane and ascorbic acid in vitro. Then the chondrocytes were implanted onto novel polymeric scaffolds made by Poly (dl-lactide-co-glycolide) embedding with chitosan nonwoven cloth. The tissue engineering cartilaginous grafts composed of chondrogenetic BMMSC/scaffolds were finally transplanted into the defects of ear cartilage in rabbits of the experimental group.

取新西兰大白兔BMMSC体外培养扩增，以含转化生长因子(TGF-β1)、地塞米松和维生素C的培养液做诱导培养，实验组以诱导后的软骨细胞与聚丙交酯－乙交酯共聚物Poly(dl-lactide-co-glycolide)包埋甲壳胺无纺布的新型支架形成复合物修复兔自体耳廓软骨缺损；对照组以PLGA/甲壳胺无纺布支架修复兔耳廓软骨缺损。

TyPe II collagen induced arthritisln the rat ank1e joint andoVathumin as antigen induced arthritis WA in the rabbit knee joint wereestab1ish2 Qualitative evaluation of me in skin, muscle, synovium, cedilagearound joint and blood was performed by OMA3 The CIA rats were treated on day 7 after hind paw swelling and erythemaAnimals were injected intravenously with ase at a dose of 10mg/kg,tWenty minuots 1ater, one ankle of the rats random1y assigned was exPosedlaser irradiation at l00J/cm fOr l000 seconds, and another ankle wasM grouP wihout laser The other two groups is unmanipulatedcontrol group and untreated CIA group Bimaleolar ankle widthmeasuremellts were taken in all animals every tWo days using amicrometer The histopathology of the ank1e Joint was assessed at day 21after disease onset4 The pro1iferating cell nuclear antigen WCNA of CIA treated by PDT andthe HMME group without laser was doterdrined by immunohistochemiStry5 The AfA rabbits were treated on day 7 after knee swelling and erythemaThe theraPy invo1ved lntravenous injection of l0mg/kg HMME, fOl1owedby 20 minues period in dim light, and transdermal light treatment with\l00 J/cm2 fOr l000 seconds The inner sides of the treated Anees wereirradiated at first, and then the outer side did 24 hours later, the synovialtissue of the Anees joint were removed and in situ cel1 aPoptosis wasdetCCted With tednal deoxync1eotidyl transferase-mediated dUTP nickend labelingR6suIt8:l The pathologic changes of CIA and AIA include subsynovial inflammation,opovial hyPerplasia, pannus formation, cartilage and bone destructionresemble RA.2 The studies demonstrated that there are different uptake of HMME withinskin, muscle, synovium, cartilage and b1ood, and the synovium cou1draPidly uPtake more ase than skin and cartilage at the firSt 30 minuesaller intravenous injection of HMME3 The bimaleolar anke width had no different among PDT treated group,H group withollt 1aser and untreated CIA group But hlstologicalevaluation showed statiStical1y significallt reductions in synovialhyperplasia, pannus formation and cart1lage reosion, bone destruction andtotal score in PDT treated group4 Image analysis showed that the ratlo bforeen the areas of the coufltedobect to that of the entire area in PDTtreated grOup is lower than that in conirol group, but the integrated oPticaldensity had no different between the two groups5 Imape analysis showed that the ratio between the area of the countedobject to that of the e

治疗组在大鼠出现踝关节红肿后1周，炎症达到高峰时进行PDT治疗。随机治疗大鼠一侧的踝关节，另。2。一一侧作单纯HMME 对照。治疗方法是大鼠麻醉后尾静脉注入 HMME10ngkg，20分钟后踝关节照光，激光波长627.sum，功率密度 100mwcm'，照射时间1000秒，能量密度100）／。治疗后避光喂养72 小时。隔日一次测量大鼠的踝关节左右横径，治疗后两周取关节进行病理d 观察。 4。大鼠CIA模型用上述方法进行PDT治疗后，治疗组和单纯HMME 组用兔疫组化SP法检测石蜡切片的核增殖抗原。 5。兔AIA模型在关节炎出现第七天进行PDT治疗，随机治疗一侧膝关节，另一侧作自身对照。兔耳静脉注入I'arrainrelomg／Kg，20分钟后，膝关节用金蒸气激光照射，激光能量密度100）儿旷。24 ／J'时后取膝关节滑膜作病理检查，并用脱氧核昔酸末端转移酶介导的缺口末端标记法原位检测凋亡细胞。结果： 1。模型观察：CIA大鼠炎症高峰期滑膜下炎细胞浸润明显，滑膜细胞明显增殖，炎症达到高峰后二周，血管缀形成，并侵蚀和破坏软骨和骨， CIA模型病理改变与人类RA相似。兔AIA模型膝关节滑膜病理可见滑膜细胞增生，滑膜下炎细胞浸润，也与人类RA滑膜改变相似。 2。关节周围组织中光敏剂含量的测定结果表明，各组织对HMME 的吸收速度和吸收量不同，荧光值一时间曲线不同，滑膜组织比皮肤和软骨对 HMME的吸收多，在 2 0分钟时即有明显差异。 3.PDT对CIA模型的治疗结果表明：PDT治疗后关节炎组、单纯 HMME组和治疗组踝关节左右横径统计学检验差异没有显著性，但病理评分PDT治疗组滑膜增生、血管资形成及软骨破坏、骨破坏和总分比关节炎对照组和HMME对照组好，统计学检验差异有显著性。。3＿军医进修学院硕士学位论文中文摘要 4.PDT治疗组PCNA阳性细胞较对照组少，图像分析结果表明面密度（阳性染色的面积总和与统计视野面积的比值）治疗组小于对照组，统计学检验差异有显著性。。 5.PDT治疗组凋亡阳性细胞较对照组明显增多，图像分析结果单位视野内阳性细胞数和面密度PDT治疗组高于对照组，统计学检验差异有显著性。凋亡细胞核直径PDT治疗组较小，与对照组相比，统计学检验差异有显著性。结论：二。CIA、AIA的病理改变类似人类RA，可作为研究RA病因、发病机制、检查及治疗方法的模型。 2。各组织对HMME的吸收速度和吸收量不同，滑膜组织比皮。

Five weeks later, comedones on rabbit in group Zi had mostly extincted, which were near to that on normal rabbit, and there was a faint or negative SP expression; In group sulfur, comedones on rabbit had a lot change and SP was positive or faint positive. While in group 0.9% normal saline nothing had happened, SP was positive.

外用药3周后紫金霜组兔耳粉刺大部分消退，基本接近正常对照兔，SP呈弱阳性或阴性；硫磺粉刺组粉刺部分减少，SP呈阳性或弱阳性；生理盐水组无明显变化，SP呈阳性。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。