免疫素

Plasma motilin, somatostatin and cholecystokinin were determined by radioimmunoassay.

运用放射免疫同批测定血浆胃动素、胆囊收缩素和生长抑素水平。

Locksley believes that AMCase, comparable to the chitinolytic enzyme found in marine and soil bacteria—and likely conserved in human evolution—attenuates the chitin-induced innate immune response by degrading the chitin.

洛克斯利相信AMCase比得上在海洋和土壤细菌中发现的甲壳素消化酶－并且可能在人类进化中保存下来了－通过降解甲壳素减弱了甲壳素诱导的先天性免疫反应。

In order to develop a safe and effective immunoadjuvant to enhance the immunity and resistance of animals against infection, a novel CpG Oligodeoxynucleotides containing 11 CpG motifs was synthesized and inserted into the VR1012 plasmid, designated as VR1C. Then the recombinant VR1C was entrapped with Chitosan nanoparticles prepared by the method of ionic cross linkage, and employed to inject muscularly 3-weeks old Kunming mice; the blank VR1012 packed with CNP and saline were used to inoculate mice as the control groups. 28 days after inoculation, all mice were orally fed with 0.4ml 2x108CFU/ per mouse virulent hemorrhagic enteritis E. coli to challenge the resistance against infection.

为研制安全高效免疫调节剂增强动物免疫抗病能力，本实验设计合成含11个C pG基序的寡聚核苷酸，重组构建含CpG的VR1012质粒(VR1C)；制备壳聚糖纳米颗粒包裹重组质粒(CNP-VR1C)，肌注接种3周龄昆明小白鼠，设壳聚糖包裹空质粒和生理盐水对照组；接种后28天口服大肠杆菌攻毒观察小鼠天然免疫的变化和对强毒感染的抵抗力，Sandwich ELISA测定血清免疫球蛋白和白细胞介素含量。

Based on these previous work, in this research, in order to explore the the molecular mechanism of enhanced tumor cell immunogenicity treated by elemene and mytomycin C compositely, we used murine Hca-F ascitic hepatic carcinoma ( high lymphatic metastasis substrain of H22) as an experimental model to compare the immunoprotective effects on Hca-F cells as well as the mechanism of the effects caused by HSP70-peptide complex purified from untreated tumor cells, EC-TCV and BCG; We studied the mechanism of increased tumor cell membrane HSP expression treated with elemene by the techniques of RT-PCR and immunoelectron microscopy; In order to deeply elucidate the antitumor effects and the molecular mechanism of elemene in increasing tumor cell immunogenicity we analyzed and compared the effects of elemene and heat shock on the gene expressions of tumor cell using gene expression profile chip.

本论文在上述研究成果的基础上，选择小鼠肝癌H22的高淋巴道转移亚系Hca-F为模型，进一步比较了未处理肿瘤细胞来源、榄香烯复合瘤苗来源的和卡介苗来源的HSP70-肽复合物对Hca-F的免疫保护效应、效应机制，探讨榄香烯-丝裂霉素复合处理增强肿瘤细胞免疫原性的分子机制；以Hca-F和人肝癌细胞株HepG〓等为模型研究了榄香烯处理增强肿瘤细胞膜热休克蛋白表达的机制；以HepG〓为模型用表达谱基因芯片研究比较了榄香烯与热休克处理对肿瘤细胞基因表达的影响，从而进一步阐明榄香烯抗肿瘤作用及增强肿瘤细胞免疫原性的分子机制。

These results show that two lectins can be used as a kind of immunity-stimulants to activate immunity system and strengthen the nonspecific immunity function of Cyprinus caarpio.

由此可看出：两种凝集素都可以作为一种免疫添加剂激活鲤的免疫系统，对鲤的非特异性免疫功能具有明显的增强作用。

Double fluorescent immunohistochemical staining revealed close apposition of VIP-, PACAP-, and vasopressin-immunoreactive terminals with tyrosine hydroxylase-ir neurons within the dorsomedial arcuate nucleus. We were then of interest to learn from where VIP-, PACAP- and AVP-containing neurons that project to the ARN originate.

经由双重免疫萤光染色法，可以在弓状核的背中区，具酪胺酸羟化酶免疫活性的细胞旁，发现有VIP、PACAP及血管加压素免疫活性的神经末稍的分布及并置，显示确有VIP，PACAP及AVP之神经元投射至弓状核。

The immune function was evaluated with T lymphocyte proliferation,NK cell activity assay,IgMplaque forming cell assay,blood serum erythrocytolysin assay and delayedtypehypersensitivity to SRBC.

采用T淋巴细胞增殖和迟发型变态反应来评价细胞免疫功能，采用血清溶血素测定和抗体生成细胞检测来评价体液免疫功能，采用乳酸脱氢酶法测定自然杀伤细胞活性来评价非特异性免疫功能。

After 24 hours, all rats were anesthetized again and their left common carotid arteries were cannulated to facilitate blood withdrawal for serum sample. The contents of tumor necrosis factor-a and interleukin-6 (IL-6) in serum were determined before and after experiment by ELISA; the activity of superoxide dismutase and content of malondialdehyde were determined by xanthinoxidanse method and modified TBA microdetermination. The content of NO2(superscript -)/NO3(superscript -), and activity of nitric oxide synthase and inducible NOS were determined by nitrate reductase method, and chemical chromogenic reaction, respectively; the immunoglobulin G, IgA, IgM and complement 3 (C3) and C4 were determined by immunoprecipitation method before and after experiment.

实验评估：手术创伤后24h进行采用酶联免疫吸附法测定实验前后血清肿瘤坏死因子α、白细胞介素6含量；应用黄嘌呤氧化酶法测定超氧化物歧化酶活性、改良TBA微量法测定丙二醛水平；以硝酸还原酶法、化学显色法分别检测NO2/NO3浓度及一氧化氮合酶、诱导型一氧化氮合酶活胜；以免疫比浊法经全自动生化分析仪检测血清免疫球蛋白IgG、IgM、IgA及补体C3、C4含量。

When the ark shell was challenged with Vibrio anguillirum, the phagocytic activity of the haemocytes increased markedly in comparison with those treated with saline or sterilized Vibrio anguillirum.

凝集素作为贝类体内参与体液免疫的非特异性免疫因子，在免疫防御活动中发挥着对异体成分进行识别、调理和促吞噬等重要作用。

ResultsIn the immune deficit mice ,1.33g/kg Naoshu capsule could enhance the phagocytic activity of monocytes, 0.67g/kg and 1.33g/kg Naoshu capsule could increase content of haemolysin antibody,and decrease footpad swelling induced by cyclophosphamide.

结果1.33g/kg脑舒胶囊能提高免疫低下小鼠单核巨噬细胞吞噬功能；0.67g/kg和1.33g/kg脑舒胶囊能增加免疫低下小鼠溶血素抗体生成，并拮抗环磷酰胺诱导的免疫低下小鼠足垫肿胀度减轻。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。