免疫素

Methods: Seven male patients with kidney stones were admitted. Abdominal subcutaneous adipose tissue and perirenal adipose tissue were collected from the operating-patients, and were cultivated with different concentrations of curcumin (10 and 100 μg/ml) in vitro.

用体外组织培养法培养手术后离体人脂肪组织，分别予10和100μg/ml姜黄素干预6h和24h，用夹心酶联免疫吸附测定法检测各脂肪组织培养液中APN和IL-6的含量。

Objective To explore the possibility of pharmacodynamic monitoring of cyclosporine immuno-suppression by measuring T-cell functions in peripheral whole blood.

目的：以不同的T淋巴细胞功能标志物作为药效动力学指标。探讨环孢素免疫抑制作用监测的可能性。

The binding of pisum sativum lectin to its receptors on normal human esophageal epithelia and changes of its distribution during neoplastic transformation were investigated by immunohistochemistry technique.

应用免疫组化技术研究了豌豆凝集素与其受体在人食管上皮的结合，以及在癌变过程中的分布变化。

Endogenous hormones, including gibberellin (GA4), indole acetic acid, isopentenyl adenine and abscisic acid, were analyzed during bolting in spring radish (Raphanus sativus L.).

采用酶联免疫吸附法测定了春萝卜抽薹过程中顶端生长点处嫩叶的赤霉素(GA4)、生长素、细胞分裂素和脱落酸等含量的变化情况。

To express chicken osteoprotegerin in Pichia pastoris and determine its inhibitive effects on mature osteoclastic bone resorptive function in vitro. The chOPG cDNA encoding for 382 amino acid without signal peptice was subcloned into Pichia pastoris expression vector pPICZα-A. The constructed plasmid was transformed into yeast X-33 by electroporation. The recombinant transformants were selected by Zeocin. Induced by the addition of methanol every 24 hours, the product analyzed by SDS-PAGE was sized about 43kDa and 53kDa at a yield of 200mg per litter of culture. The result of Western blotting indicated that the recombinant protein had specific antigenicity mainly owing to heterogeneous glycosylation.

为了在毕赤酵母中分泌表达鸡骨保护素（osteoprotegerin，OPG），采用DNA重组技术将chOPG成熟肽cDNA片段插入毕赤酵母表达载体pPICZα-A中，以电穿孔法转化酵母X-33，用Zeocin 平板筛选重组子，经甲醇诱导表达后， SDS-PAGE 和免疫印迹分析表达产物，由于糖基化不同，表达产物有两种，其相对分子量分别为43kDa和53kDa，表达量约为200mg/L，经Western印迹验证，有较好的抗原性。

Drugs containing salicylate, such as aspirin.

环孢素，一种用于抑制免疫系统的药物

Hypodrmins are good candidates for immunotherapy and for use as serodiagnostic reagents .

皮蝇素HA具有很好的抗原性和较强的免疫调节功能，是制作重组疫苗的理想候选抗原。

The N gene protein was expressed in the CHO cells proved by IFA. The N gene was transcripted as proved with RT-PCR. CHO/CDV-N cell strain was constructed, which provided evidence for research of CDV serumal detection and gene vaccine.

然后通过磷酸钙共沉淀法转染CHO-K1细胞，通过潮霉素筛选得到阳性克隆，间接免疫荧光实验鉴定N基因在CHO细胞中的表达，并用RT-PCR方法从转录水平证实N基因在CHO-K1细胞中的表达，最终建立了CHO/ CDV-N细胞株，为犬瘟热病毒的血清学检测和基因疫苗的研制奠定了基础。

After the type 1 diabetic encephalopathy rat models were set by streptozotocin for 5 days, insulin and saline were used respectively to treat the related model rats for 55 consecutive days. Morris water maze and bromodeoxyuridine immunohistochemistry were used to detect the changes in spatial memory and learning, and the neural stem cell proliferation in the subgranular zone of hippocampal dentate gyrus of each rat group.

在用链脲佐菌素建立1型糖尿病脑病模型后的第5天，分别用胰岛素和生理盐水替代治疗55天，再通过Morris水迷宫测试及5-溴脱氧尿嘧啶（BrdU，神经干细胞DNA合成的标记物）免疫组织化学方法，分别观察和比较各组大鼠SGZ神经干细胞增殖和空间学习记忆的动态变化。

There was no significance change in immunoglobulin and interleukin-1 concentration in blood after the supramaximal exercise.

超最大运动后，血液中免疫球蛋白与白血球间素-1浓度没有变化。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。