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After all the groups were intragastric administrated for 3 weeks,the lever of INHB is measured by ELISA.

灌胃三周后,用酶联免疫吸附法测量血清抑制素B的含量。

The eukaryotic expression plasmids were used as genetic vaccines to immunized intramuscularly experimental animals and post-weaning piglets.

同时还研究证明猪白细胞介素2和6基因对猪伪狂犬病核酸疫苗的免疫增效作用。

Sequences analysis indicated 26 unique cDNA sequences that encoded putative proteins showing similarities to HL35 antigen U, hlim3, heat shock protein, NADH dehydrogenase subunit 1 and calreticulin of H. longicornis, disulfide isomerase of Ambiyomma variegatum and Ixodes scapularis.

结果表明:在长角血蜱雌蜱唾液腺cDNA表达文库中获得26个长角血蜱免疫相关cDNA序列,其编码蛋白与长角血蜱HL35、黏附素hlim3假定蛋白、热休克蛋白、NADH脱氢酶、钙网蛋白以及杂色花蜱和肩突硬蜱的二硫异构酶等具有同源性。

Besides as nucleus protein, ProTαparticipate in cell multiplication and it could apply to knubbly forecasting, diagnosis and treatment, which is studied by scientists mainly.

由于在免疫和细胞生长调控方面的广泛作用,ProTα在其被发现后的20多年里,一直作为胸腺素家族中的研究重点而备受人们的关注。

In this paper, we studied seasonal variation of endogenous IAA、 GA_3、ZR and ABA content of Leymus chinensis、 Agropyron michno、 Stipa grandis、 Cleistogenes sguarrosa、 Koeleria cristata、 Carex korshinskyi、 Allium bidentatum、 Artemisia frigida, Kochia prostrate and Potentilla acaulis in different restoring succession stages, which were determined by ELISA in the growing season in 2005. At the same time we recorded the phenological development and growing regulation of each kind of plant.

本文于2005年生长季,对处于不同恢复演替阶段的羊草、米氏冰草、大针茅、糙隐子草、草、黄囊苔草、双齿葱、冷蒿、木地肤和星毛委陵菜十种植物,运用酶联免疫吸附法,测定内源生长素、赤霉素(GA_3)、细胞分裂素和脱落酸含量的季节动态,同时记录每种植物的物候期和生长规律情况。

Objective:To explore the effect of PAA and IL-2 enhanced immunoconstitution of the lethally irradiated mice following syngeneic bone marrow transplantation.

目的:为了探讨多抗甲素PAA与IL-2对骨髓移植小鼠免疫功能重建的影响。

Methods Models of leucocytopenia induced by irradiation were prepared by 60Coγ-ray and by cyclophosphamide injection. The shengbaiyin was administrated orally. The white blood cell counts, reticulocyte count, the number of nucleated bone marrow cells,T cell transformation indices, activity of tunmor necrosis factor and interleukin-6(IL-6) in blood of leukopenia mice on the 5th, 10th, 15st day after chymisty therapeutics were checked.

用60Coγ照射和注射给予环磷酰胺的方法形成骨髓抑制和免疫低下小鼠模型,中药生白饮合剂给予口服,以外周血白细胞、骨髓有核细胞、淋转指数以及细胞肿瘤因子、白细胞介素-6(IL-6)活性为观察指标。

After the culture under the mineralization fluid condition in about 1 wk, the compact circular group appeared in the intercellular space, simultaneously cell multiplication ability reduced compared with the conventional culture; The sodium alizarinsulfonate dyeing showed the compact and circular lightproof briquetting brown laminated shape in the mineralizd intercellular spac, I collagen immunity group dyeing was strongly positive in the coloring region.

在矿化液条件下培养1周左右,细胞间出现了致密圆形团,同时细胞的增殖能力较常规培养有所降低;茜素红染色结果显示矿化细胞间出现的致密的、圆形不透光团块呈现片状的棕染,着色区域, I型胶原的免疫组化染色强阳性。

Appraised the clinical effect of the flavone of rhizome drynania on Senile Osteoporosis, according to the dynamic observation on integrative scores of lumbodorsal pain and lumbodynia,"VAS section grading method" on lumbodynia,"Barthel index rating method" on ADL, detected BMD on anterior-posterior position lumbar2~4 vertebra body and proximate of femur including neck/ward/troch, and determined the index of bone transition including BGP/PINP/ICTP through the radioimmunity method before and after treated half a year, then contrast intragroup and selfgroup. Depending on the standard of curative effect, contrast the difference of curative effect between two groups.

采用随机对照的方法,分为治疗组36例,对照组34例,依据对腰背痛及腰痛综合评分进行动态观察;依据&疼痛VAS线段分级法&对腰背痛(静止痛、翻身痛、久坐痛、起身痛、弯腰痛、步行痛、睡眠痛、叩击痛、压痛)进行量化动态观察;依据&Barthel指数评定法&对老年性骨质疏松症日常生活活动能力进行观察;采用双能X线骨密度仪对腰椎(L2-L4)、股骨近端各部位:股骨颈、股骨三角区、股骨粗隆进行骨密度定量检测;应用放射免疫法对骨转换指标进行测定,骨形成指标:骨钙素、Ⅰ型前胶原氨基端前肽;骨吸收指标:Ⅰ型胶原交联羧基末端肽等,评价骨碎补总黄酮治疗老年性骨质疏松症的临床效果。

Methods The high blood sugar animal model was induced by intraperitoneal injecting streptozotocin; the MACO model was made by the thread occlusions.

采用链脲佐菌素腹腔注射复制高血糖模型;线栓法制作大脑中动脉栓塞模型;应用免疫组化技术观察MMP-9蛋白表达,并测血脑屏障的通透性。

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