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METHODS: A series of N-octyl-N'-succinyl chitosan labeled with fluorescein, were incubated with HepG2 liver cancer cells, K562 leukemia cells, A549 human lung cancer cells, and BGC cancer cells respectively and followed by using flow cytometry and enzyme-linked immunosorbent assay to assess the affinity and inhibition of N-octyl-N'-succinyl chitosan for the four kinds of tumor cells already mentioned.

用异硫氰酸荧光素对N-正辛基-N'-琥珀酰基壳聚糖进行标记,再分别与人肝癌细胞(HepG2)、人白血病细胞(K562)、人非小细胞肺癌细胞(A549)及人胃癌细胞共培养,通过流式细胞仪及酶联免疫检测仪测定N-正辛基-N'-琥珀酰基壳聚糖对肿瘤细胞的亲和性及抑制力。

This makes sense from an evolutionary point of view, since testosterone suppresses the immune system.

从进化论的观点而言,这是有道理的。因为睾丸素制约免疫系统。

Methods: Serum sICAM-1, IL-15 and monocyte ADCC of 66 Spleen-Deficiency patients were determined by enzyme-linked immunosorbent assay and modified thiazole blue test.

采用酶联免疫吸附法和改良噻唑蓝法对66例脾虚患者血清sICAM-1和白细胞介素-15(IL-15)及单核细胞抗体依赖性细胞介导的细胞毒反应进行检测。

The phenotype, calcium tuberculation and the expression of alkaline phosphatase were studied with alizarin red staining and modified Gomori Ca-Co assays respectively.

采用碱性磷酸酶Gomori 钙钴法染色、钙结节茜素红法染色及Ⅰ型胶原免疫组织化学染色方法进行鉴定成骨细胞生物学特性。

The correlation between ICJ mucosal MC counts and the number of SP positive fibers was found ( r =0.646 7, P .01), and MCs were close to SP positive fibers in lamina propria, which were demonstrated as unmyelinated nerves.

广泛分布于肠道黏膜和黏膜下层的肥大细胞(mastcell,MC)很有可能是这一机制中的一个中心环节,因为MC参与肠黏膜的免疫调节,受炎症刺激激化后,可分泌多种介质,如组胺、5 羟色胺、细胞介素

Beam walking test was used to evaluate the improvement of motor function at weeks 1,2,3 and 4 after operation. The quantity of growth-associated protein 43(GAP-43) and synaptophysin around ischemia area were examined by immunohistochemical techniques.

术后1周,2周,3周,4周应用横木行走试验评定运动功能改善,免疫组织化学方法检测缺血周围区生长相关蛋白(GAP-43)、突触素SVP)表达的变化。

The expression of survivin gene was associated with initial white blood cell count and clinical types of AL.

生存素基因的表达与白细胞总数及临床分型有关,与性别、年龄、免疫分型无明显相关性。

Methods The whole-length X gene was directly cloned to pcDNA3.1 vector. The recombinant vector (pcDNA3.1-X) was transfected into HEPG2 cells after selection with hygromycin, steady clones (HEPG2-X cells) were obtained. The expression of RhoC protein was analysed with immunohistochemical stain.

用定向克隆的方法构建X基因的真核表达载体pcDNA3.1-X,脂质体转染HEPG2细胞;潮霉素选择培养稳定表达X基因的HEPG2-X细胞;免疫组化鉴定HEPG2-X细胞RhoC蛋白表达。

The liver tissues of rats in all groups were stained by hematoxylin-eosin ,reticular fiber staining,and were employed by immunohistochemical response,and then the pathological changes, fibra accrementition and the expression of ILK andα-smooth muscle actin in rat liver tissues were observed.

对2、4、6、8周肝纤维化模型及对照组大鼠肝苏木素-伊红染色、网状纤维染色、免疫组织化学反应,观察肝组织病理变化、纤维增生及ILK和α-SMA的表达情况。

Methods: 60 SD rats were divided into 4 groups randomly, the nomal group, the model group, QZHJ group, and the hydrocortisone group. Rats were sacrificed in bdtch on the 3rd, 7th and 21st day after treatment, then the concentration of interleukin-8(IL-8) in bronchoalveolar lavage fluid and the content of transforming growth factor-β1 (TGF-β1) of lung tissue were deected, while the severity of alveolitis and fibrosis were measured.

将60只健康SD大鼠随机分为正常组、模型组、芪术合剂组和强的松组,经气管滴入博莱霉素复制肺纤维化大鼠模型,并分组予以治疗,于治疗后第3、7、21日分批处死大鼠,每组每次6只;用免疫组化法观察肺组织转化生长因子-β1的表达,并测定支气管肺泡灌洗液中中性粒细胞和白介素-8的水平。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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