免疫素

A sandwich ELISA was established taking the antiserum as the primary antibody. The sensitivity of the ELISA was 0.412 ng GH/ml of sample. Inter-assay and intro-assay coefficients of variation were 2. 58％(n=6) and 5. 69％(n=6) respectively. Serial dilutions of serum and pituitary homogenate from orange-spotted grouper yielded dose response curves that were parallel to the standard curve. And the GH of Acanthopagrus butcheri was also parallel to the standard curve. Prolactin of goldfish and recombinant growth hormone of common carp showed no cross-reactivity with the antiserum of orange-spotted grouper GH. It showed that the established RIA was specific and sensitive.

以斜带石斑鱼重组GH抗血清为第一抗体，羊抗兔抗体为第二抗体建立了双抗体夹心酶联免疫测定法，该系统的灵敏度为0.412ng GH／ml(n=6)，组内变异系数为2.58％(n=6)，组间变异系数为5.69％(n=6)，斜带石斑鱼血清和脑垂体稀释液曲线与斜带石斑鱼重组GH蛋白的标准曲线相平行、黑棘鲷GH的系列稀释曲线与标准曲线相平行，金鱼催乳素、重组鲤鱼GH的系列稀释曲线与标准曲线不平行，表明建立的斜带石斑鱼GH ELISA具有良好的特异性和重复性，灵敏度达到了测试血清样品中GH的水平。

Methods The cognitive impairment rat model was induced by hypodermic D-galactose injection and half fat feeder (6 weeks), the Morris water maze was used to screen the model rats, which were divided randomly into 4 groups (12 rats in each group): the model group, the hydrochloricdonepezil group (0.4 mg/kg, weight, sic passim), the low-dose Huannao Yicong Fang group (7 g crude drug/kg), the high-dose Huannao Yicong Fang group (14 g crude drug/kg), they were treated respectively by intragastric administration. Besides, a control group of 12 rats was set up, they were given tales doses of distilled water. After 4 weeks, the behavior of the rats was detected, the contents of plasma P-selectin and PAI-1 were measured by ELISA, also the hemorrheology was measured.

采用皮下注射D-半乳糖及喂饲半高脂饲料（6周）的方法造成大鼠认知功能障碍，Morris水迷宫行为学测试确定模型成功的动物，随机分成四组（每组12只：模型对照组给予等量水，盐酸多奈哌齐组（0.4mg/kg，体质量，下同），还脑益聪方低剂量组（7g生药／kg），还脑益聪方高剂量组（14g生药／kg），同时灌胃给药，并设立正常对照组，给予等量水。4周后，对大鼠行为学检测，应用酶联免疫法检测大鼠血浆P-选择素及PAI-1含量和血液流变学各项指标。

Paulownia A hormone has anti-inflammatory, analgesic, enhance immunity, reduce blood sugar, and low toxicity.

泡桐甲素具有抗炎、镇痛、增强免疫、降低血糖作用，而且毒性小。

Generally, interleukin-1β(IL-1β) is synthesized by phagocyte leukocyte cells and plays an important role in the immune and peripheral inflammatory response.

一般认为，白细胞介素-1β（IL-1β）是由巨噬细胞产生并在外周的免疫和炎症反应中起重要作用。

Methods The rabbit model with hyperhomocysteinemia was established by L-methionine subcutaneouse injection. The changes of white microthrombus and microvascular diameter in pia mater microvessel were observed by using cranial window method and closed microcirculation detection system, The concentration of Hcy was detected by high performance liquid, The level of von Willebrand factor, endothelin-1 (ET-1) were assayed by ELISA. The content of NO was measured by nitrate reduction method. Meanwhile, the relationship between the changes of microvascular diameter and blood concentration ET-1, NO was analyzed.

采用L-蛋氨酸皮下注射法建立HHcy家兔模型，应用"颅骨窗口法"和微循环闭路检测系统观察其软脑膜微血管中白微栓数量及微血管口径变化；采用柱前衍生反相高效液相色谱－荧光法检测同型半胱氨酸浓度；平行观测血管内皮功能指标血管性血友病因子抗原（vWF，用酶联免疫吸附法）、血浆内皮素－1ET-1，用ELISA）、血清一氧化氮水平；并对HHcy所致ET-1、NO血液浓度变化和微血管口径变化进行相关分析。

In order to investigate the effect of IFN-γon the expression of TNF-αin the hypothala- mus-pituitary-ovary axisof RU486-induced aborted SD rats, RU486 was used to establish the abortion model, then the aborted rats were administrated with different doses of IFN-γintraperitoneal injection, the method of immunohistochemical ultrasensitive SP was us- ed to study the variation of positive TNF-αcells in hypothalamus, pituitary, ovary and uterus in normal pregnant group A, aborted model group B and three different doses of IFN-γgroup C,D,E. The main goal of this research was to study whether the low dose of IFN-γcould have a protective function to pregnancy and the mechanism of its function so as to provide morph- ologic basis for the scientific using of IFN-γin the process of animal abortion control.

为探讨IFN-γ对流产大鼠下丘脑-垂体-卵巢轴(hypothalamus-pituitary- ovary-axis，HPOA )TNF-α表达的影响，本试验用米非司酮(RU486)建立SD大鼠流产模型，通过腹腔注射不同剂量IFN-γ，采用超敏感免疫组织化学SP法(链霉素抗生物素—过氧化物酶法)，观察了孕13天(d13)SD大鼠正常妊娠A组、流产模型B组、不同剂量IFN-γC、D、E组下丘脑、垂体、卵巢、子宫TNF-α的表达变化，探讨低剂量IFN-γ对妊娠是否有保护作用及其作用机制，期望能为在妊娠过程中如何科学应用IFN-γ防止动物流产提供形态学依据。

Methods Blood samples were collected from the precaval vein of 6 female Tibet mini-pigs to test the concentration of sex hormones including T,P4,E2,FSH,LH,PRL and blood biochemical indexes including Ca, Mg, P, ALP, Cre, CHO, TP, and Alb during the estrous cycle.

对6头雌性西藏小型猪在发情周期内的发情当天、第5天、第10天、第15天、再次发情时分别采血5 mL并分离血清，应用放射免疫法测定血清睾酮、孕酮(P4)、雌二醇(E2)、促卵泡生成激素、促黄体生成激素、泌乳素；用自动生化仪测定血清钙、镁、磷、碱性磷酸酶、肌苷、总胆固醇、总蛋白、白蛋白。

Abstract］ Objective: To investigate the effect of polysacchaide of silkworm pupa on immunological function in mice.

目的：通过测定蚕蛹多糖对小鼠淋巴细胞增殖、巨噬细胞吞噬活力及溶血素生成的影响，研究蚕蛹多糖对小鼠免疫功能的影响。

Objective: To investigate the effect of polysacchaide of silkworm pupa on immunological function in mice.

目的：通过测定蚕蛹多糖对小鼠淋巴细胞增殖、巨噬细胞吞噬活力及溶血素生成的影响，研究蚕蛹多糖对小鼠免疫功能的影响。

The results were as follows:(1) The CGRP- positive fibers were found in the middle and caudal part of the striatum, and located between the caudate putamen and pallidum, corresponding to the MrD of the striatum.

应用免疫细胞化学ABC法对降钙素基因相关肽在大鼠纹状体边缘区内的分布以及与杏仁核、终纹床核等边缘系统结构之间的联系进行了研究。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。