免疫素

At 0,3,6,9 and 12 weeks after infection,the ELISA was used for the quantitative determination of IFN-γ and IL-4 produced by cultivated spleen cells in vitro after stimulated by soluble worm antigen preparation,soluble egg antigen and Concanavalin A.

为进一步探讨新模型小鼠抗再感染的细胞免疫机制，该研究观察了新模型小鼠脾细胞在体外培养条件下，经可溶性成虫抗原、SEA及刀豆素A刺激后Th1细胞因子和Th2细胞因子IL

Objective:Interleukin-12(IL-12) is an important cytokine in the immunologic response when the body suffers from an fungal infection,but there are few researches in the effect of Cryptococcus Meningitis pathogenesis.

目的：白细胞介素12(IL-12)是真菌感染时机体免疫应答中发挥重要作用的一种细胞因子，但于新型隐球菌性脑膜炎发病机制中的作用研究较少。

The result of immuno-electron cytochemistry showed the number of positive reaction cells of gastrin increased significantly.

胃泌素免疫电镜细胞化学观察结果显示阳性细胞数量明显升高。

Methods: Introduce cytopathology experimental methods, human hepatic cancer cell line Bel-7402 co-cultured with Triptolide (10 g/mL, 5μg/mL and 0.25μg/mL), Using MTT method to test the effect of Triptolide on Bel-7402 cell proliferation, using TUNEL technique to test the effect of Triptolide on Bell-7402 cell apoptosis, immunohistochemistry staining method to test Bax and Bcl-2 protein expression.

采用细胞药理学实验方法，人肝癌细胞株Bel-7402与雷公藤甲素（10μg/mL、5μg/mL及0.25μg/mL）共培养，MTT法检测其对Bel-7402细胞增殖的影响，TUNEL技术检测其对Bel-7402细胞凋亡的影响，免疫组织化学染色法检测Bax及Bcl-2蛋白表达。

Objective To investigate the immunostimulation and the depressant effects of rhIL-24 protein on angiopoiesis in vitro.

目的 探讨人重组白细胞介素－24(rhIL-24)蛋白体外免疫刺激功能和血管形成抑制作用。

METHODS: A limb ischemia-reperfusion injury model in rats was established. The animals were randomly divided into three groups: control group, IR group and IPC group. The contents of diamide oxidase, nitric oxide, endothelin-1 (ET-1) and ratio of nitric oxide/endothelin-1 (NO/ET-1) in the plasma and the gut were measured. The leavels of myeloperoxidase, ratio of DNA chain, total nitric oxide synthase, inducible nitric oxide synthase and constitutive nitric oxide synthase in the gut were determined.

雄性Wistar大鼠18只，随机分为对照组，缺血再灌注组和缺血预适应组，每组6只，分别测定血浆和小肠组织二氨氧化酶、一氧化氮、内皮素-1(ET-1)、NO/ET-1比值的含量变化及小肠组织的髓过氧化物酶、DNA双链百分率(ratio of DNA chain %)、总一氧化氮合酶、诱导型一氧化氮合酶、结构型一氧化氮合酶的水平；免疫组化法检测小肠组织的诱导型一氧化氮合酶、内皮型一氧化氮合酶的表达。

RESULTSdiabetic rats showed typical symptoms involving elevated serum glucose and weight loss (2)The rats in DM+VaD group reacted slower and made more error numbers in Y-maze than the rats in VaD group did, with a prolonged total reacting time in a whole day.(3)More neurons with serious damages, such as edema, ischemia or pyknosis could be found in DM+VaD group than in VaD group. More disarrangement of cone neurons, obvious glia proliferation, and more neuron apoptosis were found in CA area of hippocampus. CONCLUSIONS (1)2-VO in STZ-induced diabetic rats succeeded in establishing VaD animal models.(2) The cognitive dysfunction induced by VaD rats had been deteriorated by diabetes mellitus.(3)Morphology evidences proved that diabetes mellitus aggravated brain damages induced by VaD.PART II Effect of diabetes mellitus on the cholinergic nervous systemin CA1 area of hippocampus of VaDOBJECTIVE To estimate the role of cholinergic nervous system in hippocampus during the process of cognitive dysfunction aggravated by diabetes mellitus in DM+VaD group. METHODS Observe the changes of ChAT protein by immunohistochemistry stain. Chose three rats randomly from each group at each pointat two week, four week, and eight week, then sepa

经腹腔注射链脲佐菌素诱导慢性实验性糖尿病，1周后永久性结扎双侧颈总动脉(2-VO)制作VaD模型记录术后2周、4周和8周各组大鼠的体重及血糖；应用Y-迷宫检测空间定向学第二军医大学博士论文中…丈摘…要1ia；一色恤~﹁染澎糕行HE染色观察组织病理学孪叱；胶质细胞原纤维酸性蛋白protellZ，Cf冰尸标记观察星形胶质细舱的激活情况、橄声软公点娜育票票众糯黑橄居端粗默孺篇筑价井袱第二部分糖尿病对血管性痴呆大鼠海马c心区胆碱能神经系统的影响探讨海马CAI区胆碱能神经系统在糖尿病加重确D一大鼠认知障碍中所实验动物及分组同第一部分，应用免疫组织化学染色方法现察海马CAI{介狱牛第晕军l袭拔常博粗{论义

Avian influenza virus ; Hemagglutinin ; DNA vaccine ; Protection efficacity

禽流感病毒；血凝素基因； DNA疫苗；免疫保护效力

At early stage after brain injury, there were a quantity of fibrous callus and cartilaginous callus formation in brain injury and fracture group and many neuropeptides immunoreactive nerve fibers in callus were found. Strong immunoreactivites of CGRP, SP, VIP, NPY, TOH occurred to osteogenitor cells and chondroblast, which proliferated in thickened endothecium.

脑损伤合并骨折组早期形成大量纤维骨痂和软骨骨痂，骨痂中神经肽免疫阳性神经纤维较多，明显增厚的骨膜内层骨祖细胞、幼稚的软骨细胞胞质内降钙素基因相关肽、P物质、血管活性肠肽、酪氨酸羟化酶、神经肽Y强阳性表达。

Methods EOM specimens were obtained from 12 patients with severe TAO and 10 healthy subjects within 1 hour after accidental death and the paraffin-embedded sections were observed by streptavidin-peroxidase immunohistochemical methods.

方法收集手术治疗的12例严重的TAO患者的眼外肌组织标本及10例健康人意外死亡1 h内取出的眼外肌组织标本，4％中性甲醛固定24 h，石蜡包埋组织，作连续切片，采用免疫组织化学链霉卵白素·过氧化物酶连接法对12例严重TAO患者及10例健康人的眼外肌石蜡组织切片进行光学显微镜观察。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。