免疫素

Further,with indirect immunohistochemistry technique,CD25(IL-2 receptor or IL-2R) cross-reaction has been identified on lymphocyte membrane of Ctenophaxyngodon idellus and Trionyx sinensis using mouse monoclonal antibody directed against Human Leucocyte Differentiation Antigen CD25(IL-2R).

进一步用抗人白细胞分化抗原CD25(IL-2受体，简称IL-2R)单克隆抗体进行免疫组化交叉反应提示草鱼、中华鳖淋巴细胞膜上含有与人类IL-2R类似功能和结构的物质。关键词：白细胞介素-2；白细胞介素-2受体；发育和比较免疫学；草鱼；中华鳖

By the affinity chromatography,fusion protein is purificated. The antiserum of chicken and rabbit were prepared with the fusion protein and Ghrelin-KLH.With active immunity and passive immunity,confirmed that Ghrelin of chicken have no obviously impact on ultimum metabolite,plasma Insulin,glucagons, T_3 and T_4.It entrances plasma GH,but inhibit food intake and increase of fat and body weight.These conclusion are obviously different from the mammal.

以纯化的融合蛋白和人工合成的Ghrelin-KLH为抗原，制备了鸡、兔抗血清：通过主动免疫、被动免疫中和实验证实禽类Ghrelin对鸡血浆中代谢产物、胰岛素、胰高血糖素、甲状腺素浓度没有明显的影响，对GH的分泌有促进作用，但与哺乳动物中不同，Ghrelin抑制鸡的脂重、体重的增加，抑制鸡的摄食。

Key words: vernonia anthelmintica flavone; melanocyte; melanogenesis; western blotting

驱虫斑鸠菊黄酮；表皮黑素细胞；黑素合成；免疫印迹

Immunoprecipitation reaction of three hemocyanin types was tested with antiserum against female specific protein and hemocyanin. It shows that the FSP and hexamer have the higher cross reaction thus they may have similar molecular structure.

利用抗FSP血清对三种型式血蓝素做扩散免疫沈淀试验，显示FSP与六隅体血蓝素有部份相似可被抗体辨识的部位，推测FSP与六隅体血蓝素的分子结构比较相近。

Methods Human luteinized granulosa cells were isolated from follicular fluid obtained during oocyte retrieval of in vitro fertilization-embryo transfer program and were cultured with M199 medium plus various concentration of leptin (0, 10, 30, 100, 300 ng/ml),human menopausal gonadotropin (hMG, 0, 0.1, 0.2, 0.5, 1, 2, 5, 10 IU/ml) and testosterone 100 μg/ml. For 2 days the media were collected for estradiol and progesterone measurements.

方法将来自体外受精-胚胎移植的卵巢黄素化颗粒细胞纯化后，在不同浓度瘦素(0、10、30、100、300 ng/ml)和人绝经期促性腺激素（hMG，0、0.1、0.2、0.5、1、2、5、10 IU/ml）单独或联合作用下进行体外培养，收集培养液，采用放射免疫方法测定颗粒细胞产生雌二醇及孕酮的量。

Methods Streptavidin-biotin complex immunohistochemistry was used to explore the expression of CD44V6 and PCNA in 35 cases of ocular squamous cell carcinomas, 20 cases of papillomas, and 11 cases of normal eyelid tissue.

方法应用链霉亲和素-生物素-过氧化物酶复合物免疫组化法检测35例眼部鳞状细胞癌、20例乳头状瘤和11例正常眼睑皮肤组织标本CD44V6蛋白表达。

The affinity constant of E1-McAb reacting with coated complete antigen was 8.2×10^8L/mol. Biotin-streptavidin complex was used for the determination of E1. The calibration curve of free E1 covered a range of 10～10 000 pg/mL.

以生物素化的羊抗鼠免疫球蛋白及辣根过氧化物酶标记的链亲和素为标记体系，通过竞争抑制的方式测定游离的雌酮，结果表明：雌酮在10～10000pg/mL内呈线性关系。

METHODS: Fasting serum levels of IL6, IL8, TNFα were measured in 30 cases of T2DM with insulin resistance and 30 cases of T2DM without insulin resistance and 26 normal control subjects by enzymelinked immunosorbent assay.

采用酶联免疫吸附法测定T2DM伴有IR患者30例和不伴有IR患者30例及正常对照26例的空腹血清白细胞介素6（IL6），白细胞介素8（IL8）及肿瘤坏死因子的浓度，并同时测定空腹血糖、糖化血红蛋白（GHbA1c）及空腹胰岛素水平，分析其与IL6，IL8，TNFα的相关性。

Methods The Biotin-Strept -Avidin enzyme linked immunosorbent assay, spectrophoto-metric assay were used to measure the plasma levels of CT-1 and sICAM-1 in 56 patients with hypertension before and after treatment of Perinopril or Benidipine and 30 normal controls.

应用生物素－链霉亲和素酶联免疫吸附法对56例高血压患者应用培哚普利和贝尼地平治疗前后及30名正常健康人，测定血浆CT-1水平和血浆sICAM-1水平。

To master fluid mosaic model of cell membrane;peripheral proteins and integral proteins;the type of extracellular matrix;Collagen;glycosaminoglycan and proteoglycan;elastin; Occliding junctions;anchoring junctions;desmosome hemidesmosome、the structure and function of adhesion belt and focal adhesion;cell coat;cadherins;selectin;Ig-superfamily; integrins

掌握生物膜的流动镶嵌模型；膜周边蛋白和膜内在蛋白；细胞外基质的类型；胶原纤维；糖胺聚糖和蛋白聚糖；弹性蛋白；封闭连接；锚定连接；桥粒与半桥粒、粘着带与粘着斑的结构与功能；细胞外被；钙粘素；选择素；免疫球蛋白超家族；整联蛋白

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。