免疫素

Prolyl isomerase, originally called cyclophilin is the target of the cyclosporin class of immunosuppressive drugs.

脯胺酸异构酶原本称为亲和素，是环孢子素这类免疫抑制药剂的目标。

Prolyl isomerase, originally called cyclophilin is the target of the cyclosporin class of immunosuppressive drugs.

脯胺酸异构酶原本称为亲和素，是环孢菌素这类免疫抑制药剂的目标。

Methods:Some immunological factors, such as IAP,IL-6 and IL-8 in serum and in prostatitic fluid were detected in 33 cases with chronic nonbacteric prostatitis, and 16 control cases.

对33例慢性非细菌性前列腺炎患者进行血清和前列腺液免疫抑制因子、白细胞介素6(IL-6)、白细胞介素8(IL-8)的检测作为病例组，并对16例健康男性也进行此三项指标检测作为对照。

HE's photomicrographs were analyzed for morphology. The proteinic expression of cyclophilin A was observed by immunochemistry. The mRNA's amount of ICAM-1 and P-selectin were quantitated by real-time quantitative reverse-transcript PCRs. All replicate data are expressed as mean±SEM.

每组随机取4例标本通过HE染色切片进行血管形态学分析，并使用免疫组织化学染色方法分析亲环素A在蛋白质水平的表达情况；另每组随机取7只小鼠通过实时定量反转录方法检测ICAM-1与P-选择素mRNA的表达。

MTA was used to manage clinical problems. These included pulpless permanent teeth with incomplete root formation and perforation in roots or furcations. Total 10 cases (12 teeth), former 7 cases (7 teeth) and latter 4 cases (5 teeth), were present. The follow-up periods were various from 6m to 21m.

应用免疫组织化学手段，观察细胞外非胶原蛋白骨唾蛋白（bonesialoprotein，BSP）、骨桥素（osteopontin，OPN）和骨钙素（osteocalcin，OC）在根尖周组织愈合期间的表达，尤其是在与倒充填材料直接相邻区域的表达。

Objective To study the relationship between expression of peripheral blood survivin and prognosis of breast cancer patients.Methods Expression of peripheral blood survivin of breast cancer was determined by the methods of RT-PCR、quantitied PCR and immunohistochemistry.

目的 探讨乳腺癌患者外周血中生存素表达的情况与预后的关系方法应用RT-PCR、定量PCR、免疫组化法检测，检测10例晚期乳腺癌患者外周血及组织中生存素的表达。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

These results indiacated that tranferring macrophages combinedwith cytokines could be a new adoptive immunotherapy protocol foradvanced cancer,and rTNF used in vivo locally could induce andactivate macrophages to kill tumor cells.Monocytes when activatedunderwent a series of phenotypic and functional changes includingthe expression of IL-2R which may provide an important immunoregu-latory pathway.The presence of lectin-like molecules on thesurface of monocytes and tumor cells may bring theeffector/target cells together,thus facilitating the inductionof apoptosis in target cells by triggering the production ofcytolytic factors and the modification of target of targer cell surface anti-gens.(such as HLA-DR).

综合以上结果可以得出以下结论：转输巨噬细胞并结合细胞因子的联合应用是肿瘤继承性免疫中的又一新的、有潜力的方法；抗肿瘤细胞因子rTNF可通过激活巨噬细胞在体内抑制对其不敏感的肿瘤细胞的生长；人单核细胞在一定条件下激活后，可表达IL-2R，进而一方面增强其自身对IL-2的敏感性，另一方面在体内也具有调节T细胞功能的作用；单核细胞和肿瘤细胞上存在的凝集素类受体可促使两种细胞之间在凝集素介导下相互接近，并诱发单核细胞产生细胞毒因子，以及通过调节靶细胞上表面抗原的表达，促进诱导靶细胞的细胞凋亡。

Methods Plasma thyroxine (T4, T3) and thyrotropic stimulating hormone concentration were measured in 78 patients with CKD (including before treatment, catabatic patients after treatment, uncatabatic patients after treatment for 8 days, then added small dose of L-thyroid hormone for 4-6 weeks) by radioimmunoassay.

对78例CKD患儿进行分期（入院治疗前，患儿达到完全缓解后，住院治疗8周确定患儿未缓解时口服小剂量L-甲状腺素片4~6周），于清晨采取其空腹静脉血2~3 mL，血标本均采用放射免疫分析法检测其总甲状腺素(T4)、三碘甲状腺原氨酸(T3)、促甲状腺激素等指标，观察其变化。

METHODS: Osteoporosis model was prepared with injection of dexamethasonc. Antagonism osteoporosis acupoint paste on acupoints and non-neridian places was administrated to compare with oral recipe for kidney tonification in the treatment of osteoporosis. It took the changes in serum estrogen, bone density, calcitonin/parathyroid hormone, thyrotropic hormone, free T3 and free T4 as the measuring indexes. Immunocyto-chemistry stain and morphological measurement of TSH cell,follicular adenoma of thyroid gland and thyroid globulin were applied in pituitary gland and thyroid gland to observe the therapeutic results.

采用注射地塞米松建立骨质疏松模型，用抗骨松穴位贴剂(antagonism osteoporosis acupoint paste，AOAP)外贴穴位、非经非穴位、并与口服补肾方药比较治疗骨质疏松，以血清雌激素、骨密度、降钙素/甲状旁腺素、促甲状腺激素、游离T3、游离T4的变化为衡量指标，取垂体、甲状腺作促甲状腺激素细胞、C细胞和Tg等免疫细胞化学染色，并形态计量，观察其治疗效果。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。