免疫的

RSB-66 was obtained from the SSH library of round spermatid-specific cDNAs against those of primary spermatocyte.

尽管FcαRI也是一种免疫球蛋白的Fc受体，但是它却和其它免疫球蛋白的受体有很大的不同，其它免疫球蛋白的FcRs大都定位在1号染色体的q21-23，上，而FcαRI却定位在19号染色体的q13.4，这个区域因为定位了几个包括杀伤细胞抑制受体和白细胞Ig样受体的白细胞受体家族而被称为

The recombinant proteins were purified according to the protocol of Ni-NTA Agarose and detected by SDS-PAGE, and TLC. Western blotting was used to identify their immunoreactivities with the serum of patient with clonorchiasis.

寄生性蠕虫以胞质GSTs为主，参与对由虫体代谢和宿主免疫系统所产生的脂质过氧化物和细胞毒性的羰基的解毒，对虫体的生存起重要的作用，是免疫疗法、化学疗法和免疫诊断潜在的靶标。

In order to investigate the role of ICAM-1 and VCAM-1 in the pathogenesis of glomerulonephritis, ICAM-1 and VCAM-1 expression were evaluated from both protein and gene level by in vivo and in vitro study, We have conducted (1) immunocytochemical analysis and in situ hybridization to examine the alteration in expression of ICAM-1 and it's relationship with interstitial infiltrating cells and TNFα; A total of 64 renal biopsies were classified in three groups according to the degree of cellular proliferation and infiltration in glomerulus;(2) indirect immunofluoresence and immunoblotting. methods to detect the VCAM-1 level both in renal tissue and in serum from the patients of lupus nephritis (17cases) and crescentic nephritis (4 cases);(3) Cell ELISA and northern blot technique to study the effects of TNFα and IL-1β on ICAM-1 and VCAM-1 surface expression and gene expression by cultured human mesangial cells .

为了探讨ICAM-1和VCAM-1在肾小球疾病中的作用，本文从蛋白质和基因两个水平，整体（研究ICAM-1时根据肾小球内细胞增生和浸润程度，将64例病人分为A.B.C三组）和细胞培养两个方面，做了如下工作，（1）利用免疫细胞化学和原位杂交技术观察了ICAM-1在64例肾小球疾病患者中的表达及其与间质浸润细胞、TNFα之间的关系；（2）利用间接免疫荧光和膜免疫印迹方法检测了VCAM-1在17例狼疮肾炎和4例新月体肾炎病人肾组织及血清中的表达；（3）利用细胞ELISA和Northern杂交技术研究了IL-1β或TNFα对体外培养的人肾小球系膜细胞ICAM-1和／或VCAM-1表面表达及mRNA表达的调节作用。

Considering, it is limited the cross-reactivity displayed by a single HVR peptide. We designed to ligate several high reactive HVR1 genes, and the recombinant fused protein containing several HVRls would have the ability to cross-react with most of HCV infected sera, and could be an effective immunogen of HCV vaccine.

但我们认为通过一条肽获得的交叉反应毕竟是有限的，故设想如果能将几个具有高交叉免疫反应性的HVR1片断用基因重组的方法连接起来，可能会获得与绝大多数病人血清发生免疫交叉反应性的抗原，并为疫苗的研究提供真正有效的免疫原。

In addition, the influences of BIS on the activities of lymphocytes in vivo and in vitro were studied, including the blast transformation of lymphocytes, the number of acid α-naphthyl acetate esterase positive lymphocyes, the cytostatic effect of hepatic LGL against hepatoma cells, in older to provided the most adequate scientific foundation for the best application of hepatoma immunotherapy.

此外还对上述不同免疫制剂在体应用对正常大鼠血淋巴细胞转化率及酸性非特异性酯酶阳性淋巴细胞数量的影响；不同免疫制剂对分离培养的肝大颗粒淋巴细胞抑肝癌细胞增殖的直接影响的研究，为肝癌免疫治疗的更佳选择提供更充分的科学依据。

Thus our study indicates that impaired immunity against nontyphoidal Salmonella bacteremia in HIV infection results from excess inhibitory antibodies against Salmonella LPS whereas serum killing of Salmonella is induced by antibodies against outer membrane proteins.

他们的发现意味着HIV所造成的缺陷不止是细胞介导的免疫，而体液免疫（是由分泌的抗体所介导的）在以LPS蛋白为标靶的免疫反应中也受到了相当大的损害。

In contrast with mucous cells, the lymphocytes in spleen and pronephric kidney performed positive for the mRNA of IgM VH detection.

上述观察表明，鲤鱼的皮肤粘液中存在的大量的免疫球蛋白是由粘液细胞分泌的，但是，粘液细胞自身不具备合成免疫球蛋白的能力，其细胞中的IgM要依赖于体内的免疫细胞。

METHODS:The gene of human ET1 was synthesized according to the preferential codons of E. coli, cloned into the EcoRI and SalI sites of vector pThioHisA. The recombinant plasmid pThioHisA-ET1 was constructed , sequenced and transformed into E.coli TOP10. Induced and expressed fusion protein were identified and analysed by 12% SDS-PAGE and densitometry analyses. After the elution, denature and renature, the fusion protein Thioredoxin-ET1 was obtained by ProBondTM chromatogragraphy. The purity of Thioredoxin-ET1 was detected by HPLC. Inoculate Thioredoxin-ET1 once per mouse every 2 weeks in 25, 50 and 100μg separately on 3 groups for 4 times. 10 days after last inoculation, we obtained venipuncture blood.

根据人ET1的多肽序列合成ET1基因，将其插入到pThioHisA的EcoRI和 SalI位点，重组质粒pThioHisA-ET1进行酶切鉴定及序列测定验证后转化TOP10，IPTG诱导的重组菌经SDS-PAGE检测融合蛋白Thioredoxin-ET1的表达量；表达的融合蛋白用ProBond亲合层析纯化并经HPLC鉴测其纯度；每只小鼠按25、50、100ug/次剂量的Thioredoxin-ET1每两周免疫一次，共4次，最后一次免疫10d后制备抗血清，经Western blot和ELISA检测证明Thioredoxin-ET1融合蛋白具有ET1免疫反应原性。

The key problem for limb , skin or other composite tissues transplantation is to control posttransplantation rejection , which encumbers long term functional survival of transplanted organ and tissues .

作为皮肤复合组织移植的受者，术后出现排斥反应的几率很高，排斥反应发生的程度和频率将直接影响移植组织能否长期存活及功能能否及时恢复，而免疫抑制剂的个体化用药是否合理又直接影响术后免疫排斥反应发生的频率和程度，因此为受者&量体裁衣&制定免疫抑制剂术后个体化给药方案，是保证移植组织或器官有功能性的长期存活的关键因素之一。

To elucidate the molecular basis of the attenuation ofDLA-EIAV in virulence thus provide theory foundation for designing HIV vaccine, thewhole genomes of DLA-EIAV and EJAV L provirus were cloned and sequenced. Aninfectious molecular clone derived from DLA-ELAV was constructed and characterized.Three DLA-EIAV/ L chimeric viruses were constructed. Promoting efficacy of the longterminal repeat of the DLA-EIAV was charactenzed.

为了揭示我国马传贫弱毒疫苗毒力致弱及免疫保护的分子机制，为人免疫缺陷病毒及其它慢病毒的免疫预防提供借鉴，我们对马传贫驴白细胞弱毒及其亲本马强毒EIAV L株前病毒进行了全基因克隆和序列测定，比较分析了二者的前病毒基因组核苷酸序列，在此基础上构建了EIAV驴白细胞弱毒株的感染性分子克隆和三株含有EIAV强/弱毒嵌合病毒基因组的重组质粒，并对EIAV弱毒株长末端重复序列的启动子活性进行了初步研究。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。