免疫的

The second part of the study examined the distribution of type Ⅰ and Ⅲ collagens, fibronectin and laminin in murine liver using protein A gold silver tealnique.

研究的第二部分建立了光镜水平的葡萄球菌A蛋白金银染色法并将其运用于对肝脏基质抗原FN和LN的免疫细胞化学研究，获得了优于免疫荧光法和免疫酶法的结果。

In summary, under the circumstances that protect of swine plague vaccine for sow, the differenceof immune response effect between advanced immunity and normal immunity is not quiet. This willprovide evidence and reference not only for illuminate the reaction mechanism of humoral immunity,mucosa immunity and cell immunity reduced by swine plague vaccine, but also for laboratory reseach,immunoprophylaxis and integrate control.

这将为进一步阐明猪瘟疫苗免疫所诱导的体液、黏膜和细胞免疫反应机制，进而为猪瘟的试验室研究及目前猪瘟的免疫预防及综合防治提供一定的理论依据和参考。

Objective To screen the protective antigen of G.intestinalis and set the foundation for immune reseach of vaccine. Methods The trophozoite antigens were obtained with in vitro culture system of Giardia lamblia Stile. After further separation, purification and activity verification, the antigens were used to immunize the BALB/C mice divided into different groups,then indexes associated with immunity were determined. Through challenge infection, the most protective antigenic component of G.intestinalis were selected based on its anti-infection ability.

目的 筛选蓝氏贾第鞭毛虫的保护性抗原，免疫疫苗的研制奠定基础方法利用已建立的蓝氏贾第鞭毛虫滋养体体外培养系统，获取滋养体抗原，并进步分离、纯化、活性鉴定；然后分组免疫小鼠，检测相关免疫指标；再行攻击感染试验，视其抗感染能力，筛选出保护性强的抗原组分。

The challenge of achieving improved monitoring of all transplant patients may allow tailoring of immunosupression in a proportion of recipients thereby increasing the opportunities for the induction of specific unresponsiveness to donor alloantigens in the future.

未来的挑战是，对移植患者的免疫系统进行良好的监测，应用个体化的免疫抑制方案，从而增加诱导针对供体同种抗原特异性免疫无反应性的机会。

In addition, the volume of milk in the immune substances, such as immunoglobulins, immune cells, lysozyme activity, as well as AKGs (ie alkoxy glycerol, breast milk is the milk AKGs content 10 times) and so on, did not smell and cattle colostrum to enhance your baby's resistance to a similar role in human milk.

此外，人乳中的在量免疫物质，像免疫球蛋白、免疫活性细胞溶菌酶，以及AKGs(即烷氧基甘油，母乳中的AKGs含量是牛奶的10倍)等，未闻及牛初乳对增强孩子的抵抗力有类似人奶的作用。

More recent evidence suggests that antimicrobial peptides are effective adjuvants, are synergistic with other immune effectors, initiate the adaptive response, support wound healing, induce or modulate of chemokine and cytokine production, alterate of gene expression in host cells, and inhibit of proinflammatory responses of host cells.

近来，越来越多的证据表明抗菌肽是有效的免疫辅助因子，能够与其他的众多免疫效应子协同作用，从而起始适应性免疫，促进伤口愈合，抑制前炎症反应以及诱导和调节细胞因子和趋化因子的产生。

Double fluorescent immunohistochemical staining revealed close apposition of VIP-, PACAP-, and vasopressin-immunoreactive terminals with tyrosine hydroxylase-ir neurons within the dorsomedial arcuate nucleus. We were then of interest to learn from where VIP-, PACAP- and AVP-containing neurons that project to the ARN originate.

经由双重免疫萤光染色法，可以在弓状核的背中区，具酪胺酸羟化酶免疫活性的细胞旁，发现有VIP、PACAP及血管加压素免疫活性的神经末稍的分布及并置，显示确有VIP，PACAP及AVP之神经元投射至弓状核。

But the major shortcoming of these vaccines is the disability to induce cellular immunity, which is essential for eliminating the endocellular virus and breaking immunologic tolerance which exist widely in chronic hepatitis B patients.

但此类疫苗最大的缺点是不能诱导有效的细胞免疫，而细胞免疫在清除细胞内病毒，以及打破慢性乙肝患者持续的免疫耐受状态具有极其重要的作用。

The number of CD〓 and CD〓 lymphocytes subpopulation and IgA, IgM, IgG antibody-producing cells, the ratios of CD〓 and CD〓 T-cell in the cecal tonsils and Harderian glands, the content of IgA, IgM, IgG in tear, tracheal fluid, intestinal fluid and bile of CIAV-infected chicks vaccinated with ND vaccine were significantly decreased compared with vaccinated control chicks 7～28 days PV.

CIAV感染雏鸡ND免疫后28天内，哈德尔腺、盲肠扁桃体以及泪液、气管液、肠液、胆汁的上述各项免疫学指标，均不同程度的明显低于未感染CIAV的ND免疫雏鸡，表明CIAV感染雏鸡呼吸道和消化道局部粘膜对ND疫苗的细胞和体液免疫应答机能下降。

PartⅡThe mechanism elucidation about effects ofα-(2,3)/(2,6)sialic acid on the Cx43gap-junction functions.(1) Westernblotting experiment showed that the decrease of sialic acid didn\'t changethe Cx43 expression and its phospholation level.(2) Westernblotting experiment showed sialidase didn,t change the ZO-1 expression,IP and confocal experiment showed sialidase improved the interaction of Cx43 andZO-1.(3) Westernblotting experiment showed sialidase didn\'t change N-cadherin expression,IP and confocal experiment showed sialidase promoted the complex formation ofCx43 and N-Cadherin.(4)Sialidase could increase the ERK1/2 phospholation level,and enchanedintercellular homotypic adhesion,Immunofluorometric assay showed sialidase couldpromote the N-cadherin cluster on the membrance.

第二部分：α—(2，3)／(2，6)唾液酸对肿瘤细胞CX43间隙连接功能影响的机制研究1、Westernblotting结果表明降低细胞膜表面唾液酸并不改变Cx43的表达及其磷酸化水平：但Cx43连接斑形成增多；2、Westernblotting结果表明唾液酸酶作用后细胞ZO—1表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与ZO-1的结合；3、Westernblotting结果表明唾液酸酶作用后细胞N-cadherin表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与N-Cadherin复合物的形成；4、唾液酸酶作用后细胞内ERK1／2磷酸化水平明显增加，细胞间同质粘附增加，以及免疫荧光表明唾液酸降低后可促进N-cadherin的膜成簇。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。