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Methods: Chitosan/pVAX1-pZP3α nanoparticles was prepared and administered into each experimental mouse on weeks 0,3 and 6 by intranasal and intravaginal respectively, whereas chitosan without DNA was given to control.

制备pVAX1-pZP3α壳聚糖纳米微粒,分别滴鼻和阴道免疫10只BALB/c 小鼠,对照组免疫不含DNA的壳聚糖,第3周和第6周分别加强免疫一次,ELISA法检测小鼠血清、阴道冲洗液中抗pZP3 IgA和IgG抗体

Twenty-six eyes which appeared rejection after allograft penetrating keratoplasty between April 2003 and April 2004 in Shandong Shi'erming Eye Hospital were collected, including 16 males and 10 females, 9 cases were repeated relapsing viral keratitis, 8 cases were traumatic posterior corneal leucoma, 5 cases were corneal decompensation caused by various intraocular surgery, 2 cases were congenital leucoma, and 2 cases had other symptom. The rejection occurred at 2 month to 2.5 years after operation. Among them 19 cases were transferred to our hospital and asked adding traditional Chinese therapy because western medicine was ineffective in other hospital.

选择山东施尔明眼科医院2003-04/2008-04接诊的同种异体穿透性角膜移植后发生排斥反应病例26例,男16例,女10例,反复发作病毒性角膜炎9例,创伤性后角膜白斑8例,各种内眼手术引起角膜失代偿5例,先天性角膜白斑2例,其他2例,上皮型免疫排斥反应、基质型免疫排斥反应、内皮型免疫排斥反应均有,排斥反应发生在术后2个月~2.5年,其中19例是外院西药治疗无效而转入山东施尔明眼科医院要求加用中医治疗。

Methods Using prime-boost immunization strategy,BALB/c mice were inoculated muscularly with HIV-2 gp105 recombinant DNA vaccine and fowlpox virus.

目的:探讨应用prime-boost免疫策略进行HIV-2外膜蛋白重组鸡痘病毒与重组DNA疫苗联合免疫引起小鼠的免疫应答。

Some serum samples from ducks immunized with attenuated DPV were assayed with the ELISA kit, and the antibodies were detected first on day 6 or day 9 post-immunization in ducks vaccinated subcutaneously, orally or nasally, and still detectable on day 60 PI.

应用该试剂盒对皮下注射、口服和滴鼻免疫DPV弱毒的20日龄樱桃谷鸭血液中抗DPV特异性IgG抗体效价进行了测定,皮下免疫鸭于第6d,口服和滴鼻免疫鸭于第9d可在血清中检测到DPV特异性抗体,且至第60d时依然可检测到高效价抗体。

The whole immune enhancement of bacterial DNA adjuvants was weaker than aluminium oxyhydrogen adjuvant, but bacterial DNA adjuvants could induce Th1 immune response in vivo, activate immune cells to kill pathogenic microbe.

细菌DNA佐剂的整体免疫增强作用弱于氢氧化铝佐剂,但其能在体内诱导Th1型免疫应答,使IgG2a/IgG1比值上升,激活免疫细胞杀灭病原体。

Challenge was made after one month of immunization by vein. Re sults:The parasitemia of recombinant virus immunized monkey was 0.0 1%in first tw o days of post-challenge. No parasites were determined from third to 12th day. W hereas the parasitemia of control virus immunized-monkey and blank control monk ey rose progressively,went to 2.5%~6.0% at 6th~8th days of post-challenge, an d the t ime of parasitemia was 12~13 days.

结果: 在免疫后1个月攻虫,重组痘苗病毒免疫猴从第3天一直到第12 d的采血检查中均未发现疟原虫;非重组痘苗病毒免疫猴第3天后原虫感染率上升,第6天原虫感染率最高达到6.0%,而后原虫感染率逐渐下降,原虫持续时间为13天;空白对照猴第3天后原虫感染率也上升,第8天原虫感染率最高达到2.5%,而后原虫感染率逐渐下降,原虫持续出现时间为12 d。

The last indigenous wild poliovirus in China was isolated in 1994,and since then,the only known isolates that can cause poliomyelitis in Chinese population appear to be the Sabin oral polio vaccinerelated strains.

自从中国承诺 1995年消灭脊髓灰质炎后,每年在常规免疫口服脊灰疫苗的基础上,还增加了国家免疫日或局部地区强化免疫活动,即对 4岁以下儿童,不论既往有无服苗史一律接种OPV。

The clinic of nursing care have documented that large dose injection of gamma globulin can prevent and cure the complication of aeteria coronaria , large dose intravenous drip of gamma globulin,2g/kg,intravenous drip slowly in 8 to 12 hours, gamma globulin has two therapeutic actions including immune vicariousness and immune accommodation. It can improve the patients' IgG level of blood quickly after intravenous drip of gamma globulin.

护理临床已证明大剂量丙种球蛋白注射,可有效防治川崎病时冠状动脉并发症,大剂量丙种球蛋白静脉滴注,2g/kg,于8-12h缓慢输入,丙种球蛋白具有免疫替代和免疫调节双重治疗作用,经静脉输入后,能迅速提高患儿血液中的免疫球蛋白

Serum immunoglobulin of Amur sturgeon Acipenser schrenckii, Chinese sturgeon Acipenser sinesis and Huso sturgeon Huso dauricus which were healthy and unimmuned, was first time respectively purified by using saturation ammonium sulfate precipitation followed by Sephadex G-200 gel column chromatography. Molecular weight and partial characterization of Ig and heavy chain and light chain of Ig were measured by polyacrylamide gel electrophoresis, SDS polyacrylamide gel electrophoresis, Western blotting and Ouchterlony method.

采用饱和硫酸氨分步沉淀和Sephadex G-200凝胶层析的方法,首次分别纯化制备了健康非免疫状态下中华鲟、史氏鲟和达氏鳇的血清免疫球蛋白,并采用聚丙烯酰胺凝胶电泳、SDS-聚丙烯酰胺凝胶电泳、蛋白免疫印迹及免疫琼扩实验等方法对其Ig及Ig亚单位的分子量和部分特性进行了分析。

Three different antiserum, rabbitantiserum against formalin-killed-cells of V. parahaemolyticus zj2003 (apathogenic strain isolated from diseased large yellow croaker, from Xiangshan bay,Zhejiang province), rabbit antiserum against OMP of the bacteria and large yellowcroaker convalescent antiserum were prepared for western blotting of the OMPpreparation.

以饱和硫酸铵梯度盐析法结合琼脂糖柱sephadex G200凝胶过滤法初步纯化了大黄鱼血清免疫球蛋白,再经割胶回收法得到进一步纯化的重链片段,分子量约为75kDa,免疫新西兰兔后获得兔抗大黄鱼免疫球蛋白血清,为ELISA和Western blotting检测技术奠定了基础。

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