免疫测定

Calf thymosin fration 5 (THF 5), prepared by modified Goldstein method, was given to 7 patients with acute acquired immunodeficiency diseases, including 1 fulminant Japanese B encephalitis associated with respiratory failure: 1 fulminant infectious hepatitis associated with hepatic coma; 1 chronic active hepatitis associated with peritonitis; 1 systemic lupus erythematosus associated with herpes zoster: 1 severe heat-exhaustion associated with deep coma, bacteriemia, and decubitus ulcer: 1 chronic granulocy...

本组7例急性T细胞免疫功能缺陷病人，经用小牛FHF_后，临床症状及实验室检查初步说明，该制品具有一定的改善患者细胞免疫功能状态和抗病毒、霉菌感染的作用。本文所采用的小牛THF_是在Goldstein氏的提取方法基础上进行了改进，分子量在15，000以下，免疫电泳分析显示没有牛血清白蛋白的大分子物质，脐带血玫瑰花结测定存在活性。由于本组病例尚少，还有待进一步探讨。

objective to study the anti-tumor activity and enhancing immune effect of phellinus linteus.methods the anti-tumor activity of phellinus linteus was estimated by the longevity of eac mice,the immune effect of tumorous mice was tested by the experiment of carbon particle expurgation and the il-2 concentration in serum of tumorous mice was determined by elisa.results phellinus linteus can prolong eac mouse's life and can increase the il-2 concentration in serum of tumorous mouse but can't affect the immune effect of tumorous mouse.conclusion chinese hurb phellinus linteus possess some antitumor power for tumor-loaded mice,but further research is needed.

目的 观察桑黄对肿瘤的抑制作用及对机体免疫功能的影响。方法以小鼠艾氏腹水癌的生命延长率评价桑黄的抗肿瘤作用，以炭末廓清实验观察荷瘤小鼠非特异性免疫功能，以elisa法测定荷瘤小鼠血清il-2浓度。结果桑黄对eac荷瘤小鼠的生存时间有延长作用，对荷瘤小鼠血清il-2浓度有提高作用，对荷瘤小鼠非特异性免疫功能无明显影响。结论中药桑黄对荷瘤小鼠具有一定的抗肿瘤能力，作用的强度和作用机制的深度还有待进一步研究。

Scanning electron micrography and X-ray fluorescence spectrometry were employed to characterize the construction process of the immunosensor. Cyclic voltammetry and differential pulse voltammetry were used to study its electrochemical properties. The content of CAP was determined with competitive immunoassay.

采用扫描电镜、X射线荧光光谱表征了免疫传感器的制备过程，采用循环伏安法和示差脉冲伏安法研究了免疫传感器的电化学性质，结合竞争性免疫分析法测定了CAP浓度。

In order to develop a safe and effective immunoadjuvant to enhance the immunity and resistance of animals against infection, a novel CpG Oligodeoxynucleotides containing 11 CpG motifs was synthesized and inserted into the VR1012 plasmid, designated as VR1C. Then the recombinant VR1C was entrapped with Chitosan nanoparticles prepared by the method of ionic cross linkage, and employed to inject muscularly 3-weeks old Kunming mice; the blank VR1012 packed with CNP and saline were used to inoculate mice as the control groups. 28 days after inoculation, all mice were orally fed with 0.4ml 2x108CFU/ per mouse virulent hemorrhagic enteritis E. coli to challenge the resistance against infection.

为研制安全高效免疫调节剂增强动物免疫抗病能力，本实验设计合成含11个C pG基序的寡聚核苷酸，重组构建含CpG的VR1012质粒(VR1C)；制备壳聚糖纳米颗粒包裹重组质粒(CNP-VR1C)，肌注接种3周龄昆明小白鼠，设壳聚糖包裹空质粒和生理盐水对照组；接种后28天口服大肠杆菌攻毒观察小鼠天然免疫的变化和对强毒感染的抵抗力，Sandwich ELISA测定血清免疫球蛋白和白细胞介素含量。

Some serum samples from ducks immunized with attenuated DPV were assayed with the ELISA kit, and the antibodies were detected first on day 6 or day 9 post-immunization in ducks vaccinated subcutaneously, orally or nasally, and still detectable on day 60 PI.

应用该试剂盒对皮下注射、口服和滴鼻免疫DPV弱毒的20日龄樱桃谷鸭血液中抗DPV特异性IgG抗体效价进行了测定，皮下免疫鸭于第6d，口服和滴鼻免疫鸭于第9d可在血清中检测到DPV特异性抗体，且至第60d时依然可检测到高效价抗体。

Before the first immune and 1 week after strengthened immune, take blood and measure AsAb with ELISA method, measure the immune integral of IgG and IgA in uterus tunica intima with SABC method; meanwhile, compare the pregnant rats and average nidation points in all groups.

在首次免疫前、各次加强免疫后1周取血ELISA法测定AsAb，SABC法检测子宫内膜IgG、IgA免疫积分，同时比较各组大鼠受孕数及孕鼠平均着床点数。

METHODS: A total of 264 chronic HBVinfected patients were enrolled in our study, and were divided into lowlevel HBsAg group(147 cases) and highlevel HBsAg group(117 cases) based on the HBsAg level or were divided into immune tolerance stage, immune clearance stage and nonactive stage based on the natural history. Realtime PCR and microparticle enzyme immunoassay were used to determine the content of HBV DNA and HBV M in serum samples of highlevel and lowlevel HBsAg patients, respectively, then the quantitative results were compared.

对264例慢性HBV感染者的HBsAg浓度(高浓度HBsAg组、低浓度HBsAg组)及自然史(免疫耐受期、免疫清除期、非活动期)进行分组，采用实时荧光聚合酶链反应和微粒子酶免疫试验对147例低浓度HBsA组和117例高浓度HBsAg组的血清标本进行HBV DNA和HBV M的定量测定并进行比较。

This research determines the change that analysed patient of mobile sex tuberculosis to treat around CRP, medium clinical import is evaluated in order to discuss CRP in phthisical curative effect. 1.1 average data choose 1 data and method to come from January 2005 August 2007 the patient of mobile sex tuberculosis of my courtyard be in hospital 120, all case of illness all combine the phlegmy smear, diagnose such as germiculture according to sternum of line of clinical expression, X or bosom CT, the tuberculosis that chooses case of illness to all accord with China cure to learn tuberculosis to learn branch to make is diagnosed and treat a guideline [2] , 120 all are patient of mobile sex tuberculosis is treated first, male 78, female 18 ～ is 73 42; age years old, average age (40 ± 6) year old. 1.2 methods are all patient early morning is hollow vein collects blood, the United States is used after detached serum elegant earth up Ci8200 full automatic biochemical analyzer determines, use scattering immunity to determine than chaotic law serum CRP level, and determine at the same time red blood cell sedimentations rate , bacterium of phlegmy n/med tuberculosis is checked of education of n/med tuberculosis bacterium...

本探究测定分析了活动性肺结核患者治疗前后CRP的变化，以探索CRP在肺结核疗效评估中的临床意义。1资料和方法1.1一般资料选自2005年1月至2007年8月我院住院的活动性肺结核患者120例，所有病例均根据临床表现、X线胸片或胸部CT结合痰涂片、细菌培养等确诊，所选病例均符合中华医学会结核病学会分会制定的肺结核诊断和治疗指南[2]，120例均为初治活动性肺结核患者，男78例，女42例；年龄18～73岁，平均年龄(40±6)岁。1.2方法所有患者清晨空腹静脉采血，分离血清后采用美国雅培Ci8200全自动生化分析仪测定，采用散射免疫比浊法测定血清CRP水平，且同时测定红细胞沉降率，痰结核菌检查。。。

METHODS:The gene of human ET1 was synthesized according to the preferential codons of E. coli, cloned into the EcoRI and SalI sites of vector pThioHisA. The recombinant plasmid pThioHisA-ET1 was constructed , sequenced and transformed into E.coli TOP10. Induced and expressed fusion protein were identified and analysed by 12% SDS-PAGE and densitometry analyses. After the elution, denature and renature, the fusion protein Thioredoxin-ET1 was obtained by ProBondTM chromatogragraphy. The purity of Thioredoxin-ET1 was detected by HPLC. Inoculate Thioredoxin-ET1 once per mouse every 2 weeks in 25, 50 and 100μg separately on 3 groups for 4 times. 10 days after last inoculation, we obtained venipuncture blood.

根据人ET1的多肽序列合成ET1基因，将其插入到pThioHisA的EcoRI和 SalI位点，重组质粒pThioHisA-ET1进行酶切鉴定及序列测定验证后转化TOP10，IPTG诱导的重组菌经SDS-PAGE检测融合蛋白Thioredoxin-ET1的表达量；表达的融合蛋白用ProBond亲合层析纯化并经HPLC鉴测其纯度；每只小鼠按25、50、100ug/次剂量的Thioredoxin-ET1每两周免疫一次，共4次，最后一次免疫10d后制备抗血清，经Western blot和ELISA检测证明Thioredoxin-ET1融合蛋白具有ET1免疫反应原性。

Adoping the simple and easy anthology to gather the unit to gather urine of 5 hours measures, Watch different series of urine quantitative changeization .(2) Experiment of immunity: Adopted CY methods copy immunity to repress the little rat pattern, To divide randomly small healthy white mice originated from kunming into normal contrast group ,pattern group, Fulin group, Guizhi group,Fulin and Guizhi group (Two medicine are according to 1 : 1、2: 1、 1: 2、 4: 3 the proportion compose ).Be total to 8 groups . To observed Fulin and Guizhi different combinition adjust the repressing small mice's spleen and thymus, Ascertaining by measuring little rat phagocyte the gobbleing uprate and serum IL-2 -* TNF — a 's content.

2免疫实验采用环磷酰胺法复制免疫抑制小鼠模型，健康昆明种小鼠随机分为正常对组、模型组、茯苓组、桂枝组、茯苓与桂枝配伍组(两药按1：1、2：1、1：2、4：3比例进行组合)共8组，观察茯苓桂枝药对及其各配伍组对免疫抑制小鼠脾、胸腺的影响，测定小鼠吞噬细胞吞噬率及血清IL-2、TNF—α含量。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。