免疫测定

Three different immunoglobin subtypes, alexin C3, alexin C4, blood lipid, fasting plasma glucose, and fasting plasma insulin were examined. Results The IgG, IgM, IgA and alexin C3 of simple obesity children were significantly lower than those of healthy children.

运用散射比浊法测定免疫球蛋白IgG、IgA、IgM及补体C3、C4，同时测定空腹血糖、血脂、胰岛素，两组进行比较并探讨影响体液免疫功能相关因素。

Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.

(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗；(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力；(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性；(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡；(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型，应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况，明确其靶向性；(6)将荷瘤裸鼠分3组进行放射免疫治疗，分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。

In addition, Immune nephelometry assay was imployed to analysis the level of rats' plasmic Fg, in the mean time, Platelet aggregation meter was used in observing the ratio of rats' platelet aggregation at 3 min. and 10 min, respectively.

实验采用酶联免疫吸附法测定各组大鼠血浆中TXB2、6-Ke-PGF1α、PGE2的水平；另外，采用免疫比浊法测定两组大鼠血浆中纤维蛋白原的含量，并同时观察各组大鼠3min，10min内的血小板聚集率。

Methods The serum sICAM-1 was measured by ELISA in 20 patients with pollinosis and in 2 0 healthy persons as normal control group.The serum tIgE was analyzed with the P harmacia Uni-CAP System for two groups but Artemisia pollen-specific IgE was a ssayed only for patents group.

对20例花粉症患者及20名健康人采用酶联免疫吸附实验测定血清sICAM-1，用荧光免疫法测定血清tIgE和蒿草花粉特异性IgE(W6)。

On days 7, 14 and 28 after the addition, the total and differential counts of leucocytes, lymphocyte proliferation activity, and serum contents of immunoglobulin and cytokines, as well as immune organ index, were determined.

添加后7、14和28d每组选5头采血，测定外周血白细胞分类和淋巴细胞转化活性以及血清免疫球蛋白和细胞因子含量，并屠宰测定免疫器官指数。

Methods Among 86 patients in observation group with acute coronary syndromes,there were 47 cases with unstable angina pectoris and 39 cases with acute myocardial infarction,and 40 cases of health people in contrast group.The serum level of sICAM1 and sVCAM1 were detected by ELISA,and CRP was detected by immune turbidimetry in all cases.

选取ACS患者86例为观察组，将观察组分为不稳定型心绞痛和急性心肌梗死两组，UAP组47例，AMI组39例，另选健康成人40例为正常对照组，采用酶联免疫吸附法测定各组血中sICAM1和sVCAM1水平，采用免疫浊度法测定各组血中CRP浓度，并对其结果进行比较。

Methods Twenty-two cases of nasal polyp and 10 cases of normal inferior turbinate were studied with immunohistochemical SABC method and ELISA method.

应用免疫组化和酶联免疫吸附测定法，对22例鼻息肉和10例对照组下鼻甲组织内IL-8 的分布及含量进行测定。

After Bel-7402 was treated with melittin, detection methods were applied as followed, 1 measure the change of proliferation by MTT ; 2 light microscope and transmission electron microscope were used to observe the morphous and biological behaviour and ConA agglutination test and clonality test were applied to measure the adhesion capability and growth potency of Bel-7402; 3 ELISA , colorimetry , bromocresol green mehod were used to determine AFP level, the activity of y - GT and ALP, ALb level respectively; 4 the change of c-myc gene expression was detect by immunohistochemical method.

观察蜂毒素作用于人肝癌Bel-7402细胞株后(1)MTT方法测定对肿瘤细胞增殖的影响(2)光学显微镜和透射电镜下观察对形态及生物学行为的影响，ConA凝集试验及克隆形成能力反映处理后细胞粘附能力及生长潜能(3)ELISA法测定甲胎蛋白含量、重氮反应比色法测定γ-谷氨酰转肽酶活力、比色法测定碱性磷酸酶活力、溴甲酚绿法测定白蛋白含量，检测对肝癌细胞代谢与分泌的影响(4)免疫组织化学法检测处理前后c-myc基因表达的差别。

Change in the concentration of endogenous IAA was measured with enzyme-linked immunosorbent assay, and the distribution of IAA-binding protein in the protoplasts in the cambial region was tested with an immuno-fluorescence technique, being improved by the authors, during the cambial active period in Broussonetia papyrifera L.

构树（Broussonetia papyriferaVent。）形成层活动周期中用酶联免疫吸附法测定了形成层区域的内源IAA浓度的变化，并用自行发展的免疫荧光检测方法测定了由此处生活细胞制得的原生质体中IAA结合蛋白的分布。

The titer test showed that the polyclonal antibodies immunonized by two immunogens all had high titer and the longer interval made for obtaining higher titer. The speciality test showed that the polyclonal antibodies immunonized by conjugations MQCA-RSA had the better speciality than the other one. Usage of RSA as the carrier protein could avoid producing antibody for carrier protein.

以两种偶联物为不同免疫原、采用不同的免疫时间间隔免疫新西兰大白兔，获得多克隆抗体，抗体效价测定和特异性鉴定的实验结果表明：两种免疫原均能产生高效价的抗体，免疫间隔延长有利于效价提高，且以RSA为载体有利于避免针对蛋白载体的抗体产生。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。