免疫测定

At the time of aorta opening in routine cardiac surgeries with ECC,the oxygen flux was changed respectively. Two millilitres blood was taken at the time of pre-operation（T0）,30 min after aorta opening（T1）,6 h (T2),24 h (T3) and 48 h after operation (T4) to measure the levels of serum cTnI were measured through ELISA method.

常规体外循环下进行手术，升主动脉开放时改变各组复灌氧流量，分别于术前（T0）、开放升主动脉后30 min（T1）、术后6 h（T2）、24 h（T3）、48 h（T4）各时间点抽取患者锁骨下静脉血2 ml，采用ELISA酶联免疫法测定血浆cTnI含量。

Mature and immature embryos of maize inbred line 87-1 were used as explants and calli were induced and cultured on improved N6 medium. The method of enzyme-linked immunosorbant assay was employed to detect endogenous contents of abscisic acid, indoleacetic acid, gibberellins (GA3) and cytokinins of immature, mature embryos and their calli during culture process.

以玉米自交系87-1的幼胚和成熟胚为材料，采用酶联免疫法测定了不同培养时间诱导的幼胚及成熟胚愈伤组织内源脱落酸、吲哚乙酸、赤霉素(GA3)和细胞分裂素含量的动态变化。

The dynamic variation in content of cndogenous abscisic acid,indoleacetic acid,gibberellinsand isopentenyladenodines of normal juvenilefruit and dropped fruit was measured with enzyme linked immunosorbent assayin the course of physiological fruit drop of 'Shuizhang Longan.

应用酶联免疫法测定了水涨龙眼生理落果期间正常幼果和脱落幼果中内源激素ABA、IAA、GAs及iPAs含量的变化。

The methods of enzyme-linked immunosorbant assay were employed to detect endogenous contents of abscisic acid, indoleacetic acid, gibberellins (GA3, GA4) and cytokinins of immature embryos, embryonic and non-embryonic calli during culture process.

酶联免疫法测定不同培养时间后未成熟胚，以及由此诱导的胚性和非胚性愈伤组织，内源脱落酸、吲哚乙酸、赤霉素(GA3、GA4)和细胞分裂素(DHZR、IPA、ZR)含量。

To determine the immune reactivities in kidney transplant recipients and investigate their correlation with clinical events.

目的：测定肾移植受者免疫反应性，并探讨临床事件的关系。

In severe patients, the change of CRP and cTnⅠ was higher than the mild patients.

以化学免疫荧光法定量测定血清CRP及cTnⅠ水平。

Methods Serum CTn Ⅰ and CK MB levels were determined by the chemiluminoimmunoassay and enzyme assay in 65 neonates of MAS with or without myocardial lesions (n=35 and n=30, respectively) and 15 normal newborns.

用化学发光免疫法及酶法分别测定 35例合并心肌损伤的MAS患儿、30例无心肌损伤的MAS患儿及 15例正常对照组血清CTn Ⅰ及CK MB的水平。

Serum aminoterminal propeptide of procollagen type III was quantified by RIA ( Riagnost PIIIP c.t. Biodesign, 20 controls= 0.4 ± 0.2 U/L ).

用放射免疫方法来定量地测定血清中III型原骨胶原的氨基酸末端（PIIIP的放免诊断，生物设计20个对照组=0.4±0.2U/L）。

Methods In the present study, incubation of postnatal Wistar rat cortex neurons in culture with matrine at 0.5, 1.0, 2.0mmol/L was carried out to have knowledge of the effect of matrine on the expressions of GABAR.

对Wistar新生大鼠原代培养皮质神经元分别给予剂量为0.5、1.0、2.0mmol/L培养基的苦参碱进行药物干预，4h后用细胞免疫组化方法测定GABAR的蛋白表达。

Objective To study the mechanism of the inbitory effect of matrine on central nervous. Methods In the present study, incubation of postnatal Wistar rat cortex neurons in culture with matrine at 0.5, 1.0, 2.0mmol/L was carried out to have knowledge of the effect of matrine on the expressions of GABAR.

目的 观察苦参碱对鼠原代培养皮质神经元γ-氨基丁酸受体蛋白表达的影响，探讨苦参碱抑制中枢的作用机制方法对Wistar新生大鼠原代培养皮质神经元分别给予剂量 0.5、1.0、2.0mmol/L培养基的苦参碱进行药物干预，4h后用细胞免疫组化方法测定GABAR的蛋白表达。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。