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免疫测定

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Methods The intra-dermal test and intranasal provocation test with ragweed pollen allergen, and specific serum IgE determination were given to patients with pollenosis. Results 85 patients were proved as ragweed pollensis in 502 patients with pollenosis based on the ARIA standard. They received step-treatment and specific immunotherapy.

在就诊的花粉症患者中,通过豚草花粉变应原皮内试验、鼻黏膜激发试验、血清特异性IgE测定等检测方法,按照&ARIA&关于变应性鼻炎新的分类标准进行了分类和阶梯式治疗及特异性免疫治疗。

Methods: ELISA was used to determine the serum of TPA and sVCAM-1 in pre-eclamptic and normotensive pregnant women, and the correlation of pre-eclampsia and neonatal outcomes was analyzed by rank correlation.

采用酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)测定子痫前期患者和正常晚孕妇女外周血中TPA、sVCAM-1的浓度,并与子痫前期患者病情及新生儿体重进行相关分析。

Based on pre-experiment foundation of the earlier period, 60 male patients among 45-65 year old were chosed as the clinical case according to the PADAM diagnosis standard, and were divided into 3 groups according to the block stochastic principle, the observation group (acupuncture combined with BUSEHN capsule), group 1, group 2 (takes orally BUSEHN capsule group), each group had 20 cases, and each group of patients was measured by the method of radio-immunity for the serum level of testosterone, luteinizing hormoha, follicle-stimulating hormone before and after the treatment.

在前期预实验的基础上,选择符合诊断标准的PADAM诊断标准的45-65岁男性患者临床病例60例,按照区组随机原则分为3组,观察组、对照组1、对照组2,每组各20例,采用放射免疫法分别测定各组患者治疗前后血清中血清睾酮、黄体生成素、卵泡刺激素的血清浓度的水平;通过三组及三组间治疗前后PADAM症状评分指数的对比研究,揭示针药结合的整体调节作用特色,探讨提高针灸疗效的新途径;总结出针刺对中老年雄激素缺乏综合征患者的内分泌的调节及针刺对睾丸的改善程度,探讨针药结合治疗本病的机制及针灸增效剂的应用前景,为今后进一步研究打下坚实基础。

Methods Cellular immunity detection and humoral immunity detection were made to76cases with OHS and the results were analyzed.

对76例OHS患者进行了细胞免疫,体液免测定,并对结果作分析。

Methods Expressions of Survivin and eIF4E in specimens of 40 LSCC and 20 cases with polyp of vocal cord were detected via immunohistochemical technique.

应用免疫组化法测定40例喉鳞癌组织标本及20例声带息肉组织中Survivin 与eIF4E的表达,并进行比较。

Combined with solvent extraction separation, the method has been applied to the determination of trace amount titanium in human hair, vegetable, tea and rock samples.

微量元素钛在生物体内有增强免疫能力,刺激植物生长等功能,随着环境科学和生命科学的发展,痕量钛的测定越来越受到人们的关注。

Single-color or two-color direct immunofluorescence staining were used by means of flow cytometry.

采用单标或双标直接免疫荧光标记法标记20种细胞表面分化抗原,经流式细胞仪测定。

Methods: The blood levels of insulin, glucagon and somatostatin before and on 60 minutes after ingestion of test meal were measured by radioimmunassay in 40 children with OB and 30 age-sex matched healthy children as controls.

采用放射免疫分析法测定SO患儿空腹和摄入液体实验餐6 0min时外周血胰岛素、胰高血糖素和生长抑素水平。

The jugular vein blood samples of Jining Gray She-Goat were collected during the oestrus cycle. The secretory characteristics of FSH, LH, E2 and P were measured using radioimmunoassay system.

本试验对处于繁殖季节的济宁青山羊进行颈脉采血,用放射免疫分析法测定了血浆内促性腺激素和性激素(E2、P)的含量。

Methods The experimental SAH models of Wistar rats were completed by transcranial drilling and the reperfusion of self-blood, the levels of GMP-140 and TXB2 in plasma were determined by radioimmunologic method.

经颅骨钻孔-注入自家血方法建立大鼠SAH的动物模型,应用放射免疫法对SHA后不同时间内大鼠血GMP-140、TXB2水平进行测定。

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