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免疫测定

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Methods: control group, model and the Effects of the Qishen Fukang capsule low, middle and high 0.3, 0.6, 1.2 gkg^(-1 drug group, gavage for 25 days after the administration of the immune function of normal control And model groups were given distilled water.

实验设正常对照组、模型组和芪参复康胶囊低、中、高(0.3、0.6、1.2g/kg)药物组,灌胃给药25天后进行免疫功能的测定,正常对照组和模型组均给予蒸馏水。

In order to determine the immunomodulatory effect of the dietary intake of levamisole in the Chinese mitten crab, the crab were fed diets containing 0, 100, 200 and 300 mg levamisole kg1 dry diet for 7 days. The total haemocyte count, differential haemocyte count, phagocytic activity, respiratory burst, phenoloxidase activity and lysozyme activity were examined at 0, 2, 4, 6, and 8 weeks after administration of levamisole.

中文摘要:摘要:为测定在饲料中添加左旋咪唑对中华绒螯蟹的免疫调节作用,以0、100、200和300 mg · kg1干饲料的剂量将LMS添加于基础饲料中制成颗粒饲料,投喂中华绒螯蟹7 d,然后投喂不含LMS的对照组饲料。

And we investigated the effect on humoral immunity with haemolysis plaque forming cell test.

用溶血空斑形成细胞测定法研究对体液免疫功能的影响。

In order to evaluate the effects of yeast cell wall on carp growth, immunity apparatus and muscle nutrients, carp growing performance, head kidney index, spleen index and muscle nutrients were determined after breeding for 40 days.

为评定酵母细胞壁对鲫鱼生长、免疫器官和肌肉营养成分的影响,测定饲养40d后鲫鱼的生长性能、头肾指数、脾脏指数和肌肉营养成分。

Pseudosciaena crocea were immunized with formalin-killed and heat-killed trivalent vaccines by immersion and injection, which was made up of four experimental groups.

采用0.5%福尔马林4℃灭活和65℃热灭活2种不同的方法制备三联疫苗作为抗原,以多次腹腔注射或浸泡的方式免疫大黄鱼,通过测定受免大黄鱼血清中抗体凝集效价变化并进行攻毒实验以评估保护效果。

Each group was treated by intraperitoneal injection once every two days. The accumulation and metabolism of Al was analyzed by atomic absorption spectrum, expression of ED-1 by Kupffer cell was analyzed by immunohistochemistry.

采用原子吸收分光光度法测定铝元素在肝组织中含量的变化;免疫组织化学染色观察肝组织中枯否细胞的活化;RT-PCR检测凋亡相关基因Bax及Bcl-2 mRNA表达的改变。

The cell biological features were observed by inverted phase contrast microscope, l ight microscope, electron microscope, cell vital ity assay, cell growth curve and cells staining after harvest and during the periods of culturing the primary, the 1st passage and 2nd passage.

分别在取材后、原代、第1 代、第2 代细胞培养期间,进行髓核细胞活力测定;爬片培养后进行甲苯胺蓝、HE、聚集蛋白聚糖番红O、Ⅰ型及Ⅱ型胶原免疫组织化学染色观察;MTT 法绘制髓核细胞生长曲线,并行原代及第2 代细胞透射电镜观察,对体外细胞的生物学特性进行研究。

9 healthy adult Holstein cows were selected as experimental cows and they were divided into 3 groups according to different milk lactations. Individual tracking method was used to observe and record the amount of Diptera insects. The blood was took on the tail vein under the aseptic condition and then the serum was extracted. The level of ACTH, COR, PRL, GH and NE in the serum were detected by the method of radioactive immunoassay method and fluorescence spectrophotometry.

方法]选择9头成年健康的荷斯坦奶牛作为试验牛,按不同泌乳阶段分3组,采用个体跟踪法观察并记录侵袭试验牛双翅目昆虫的数目,自尾静脉无菌采血,提取血清,采用放射性免疫法和荧光分光光度法测定血清中AGTH、COR、PRL、GH和NE的水平。

The left legs were taken for the biomechanics test on the EnduraTEC ELF3200, the biggest vertical deformation and stiffness were calculated, the number of empty osteocyte lacunas was calculated, and the cumulative absorbance value was compared between two groups.

分别于2,4,6,8周处死大鼠,取右侧股骨头标本,于EnduraTEC ELF3200生物力学材料动态力学性能测试系统行压力测定,计算样本纵向最大位移、刚度吸收等生物力学参数,计算空骨陷窝数,并行免疫组织化学法Bcl-2染色,比较不同组之间的累计吸光度值。

Methods We detected c-Myc, Bcl-2 and Fas proteins and performed immunophenotyping by flow cytometry in 21 cases of CML marrows and 9 normal bone marrows.

采用流式细胞术,测定21例CML及12例正常骨髓标本单个核细胞的髓系免疫表型及三种与PCD有关的调节蛋白――c-Myc、Bcl-2、Fas的表达。

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