免疫测定

Results:The positive expression of P53 and c－Myc protein in nuclei was bound up with poor-differentiated tumours;the positive expression of Fas and c－Myc protein in cytoplasms was consistent with well-differentiated tumours.

采用免疫组织化学方法对骨肉瘤、骨化性纤维瘤、骨纤维结构不良和正常骨痂组织进行c－Myc、Fas和P53蛋白测定，与肿瘤组织学分级、细胞分化及核丝分裂比较分析。

The serum and CSF levels of IL1β, IL6, TNFα and IL1ra in the patients with different kinds of dementias and the controls were measured using ELISA.

用夹心式酶联免疫吸附法测定各组老年人血清和脑脊液中白介素1β（IL1β），白介素6（IL6），肿瘤坏死因子α和白介素1受体拮抗剂（IL1ra）的水平。

Methods The astrocytes were isolated from cerebral cortex of neonatal rats which are born within three days, cultivating by DMEM media added with 15% fetal calf serum and depurating by swinging and passaging, to simulate nulli-nutrition model by substituting medium for PBS buffer solution 3h then applying methylprednisolone and recnutrition immediately, we will go on culturing for three days, verificating astrocytes by immunofluorescent technique; detecting activity of AST; determining GFAP of change by PCR.

采用出生3d内的SD大鼠的新生鼠的大脑制成细胞悬浊液后，应用含15%胎牛血清的DMEM培养液中培养，通过摇床和逐渐传代纯化星形胶质细胞，通过PBS缓冲液代替培养基模拟缺营养模型3h后即刻应用甲基强的松龙(10umol/l)，然后继续培养3d，免疫荧光技术鉴定星形胶质细胞；MTT法检测细胞的增殖活性和凋亡；PCR技术测定细胞GFAP表达的变化。

In order to select monomer of Chinese herbal polysaccharide with better immunity, four CHPs, APS, ABPS, IRPS and DOPS at six doses were added in the splenic lymphocyte of chick in vitro, and cultured 48 h. The splenic lymphocyte proliferation was determined with the MTT method.

为了筛选免疫增强作用较好的单味中药成分，将6种浓度的黄芪多糖、牛膝多糖、板蓝根多糖、山药多糖等4种中药多糖分别加入到体外培养的鸡脾脏淋巴细胞中，培养48h后，用MTT法测定淋巴细胞增殖的变化。

The expression of p16INK4a protein was tested by immunochemical assay, and the life span in vitro of nasopharyngeal epithelial cells was calculated as population doublings.

我们应用老化相关半乳糖苷酶染色检测SA-β-Gal活性的表达情况，并利用免疫组化测定p16INK4a蛋白的表达。

Ca2+ blocker was given in control group. Their blood glucose level, blood pressure and Ualbumin excretions were determined both in the treatment and the control group before and after the treatment.

采用免疫比浊法测定2型糖尿病合并高血压患者的尿微量白蛋白，选择34例尿微量白蛋白正常的患者，随机分为2组，治疗组17人，在口服降糖药的基础上加咪达普利，对照组17人，在口服降糖药的基础上加钙离子拮抗剂，两组治疗前后均监测血压、血糖、糖化血红蛋白和UAE。

Methods A total of 34 diabetes patients with hypertension were studied. A dose of 5 mg imidapril was given daily in treatment group except oral medicine for decreasing glucose level. Ca2+ blocker was given in control group. Their blood glucose level, blood pressure and Ualbumin excretions were determined both in the treatment and the control group before and after the treatment.

采用免疫比浊法测定2型糖尿病合并高血压患者的尿微量白蛋白，选择34例尿微量白蛋白正常的患者，随机分为2组，治疗组17人，在口服降糖药的基础上加咪达普利，对照组17人，在口服降糖药的基础上加钙离子拮抗剂，两组治疗前后均监测血压、血糖、糖化血红蛋白和UAE。

A dose of 5 mg imidapril was given daily in treatment group except oral medicine for decreasing glucose level. Ca2+ blocker was given in control group. Their blood glucose level, blood pressure and Ualbumin excretions were determined both in the treatment and the control group before and after the treatment.

采用免疫比浊法测定2型糖尿病合并高血压患者的尿微量白蛋白，选择34例尿微量白蛋白正常的患者，随机分为2组，治疗组17人，在口服降糖药的基础上加咪达普利，对照组17人，在口服降糖药的基础上加钙离子拮抗剂，两组治疗前后均监测血压、血糖、糖化血红蛋白和UAE。

A dose of 5 mg imidapril was given daily in treatment group except oral medicine for decreasing glucose level. Ca2+ blocker was given in control group. Their blood glucose level, blood pressure and U??albumin excretions were determined both in the treatment and the control group before and after the treatment.

采用免疫比浊法测定2型糖尿病合并高血压患者的尿微量白蛋白，选择34例尿微量白蛋白正常的患者，随机分为2组，治疗组17人，在口服降糖药的基础上加咪达普利，对照组17人，在口服降糖药的基础上加钙离子拮抗剂，两组治疗前后均监测血压、血糖、糖化血红蛋白和UAE。

Material and methods: Human breast cell line HBL-100 and breast carcinoma cell line MCF-7 were cultured in a medium either in the absence or presence of folacin for 30 days.

材料与方法：将人乳腺细胞株HBL-100和人乳腺癌细胞株MCF-7在叶酸缺乏的培养基和正常培养基中的培养30天，用细胞计数、FCM和电镜判断叶酸缺乏对HBL-100和MCF-7细胞生长、周期分布和凋亡状况的影响；用免疫组化和原位杂交等技术，观察叶酸缺乏对HBL-100P53蛋白表达与mRNA表达的影响；用半定量PCR的方法，测定了叶酸缺乏对HBL-100 P53外显子6、7、8甲基化水平的影响。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。