免疫测定

The maxillofacial and brain injuries were examined. Craniptomy was done to animals of impact injury 6 hours after damage, however it was done to animals of explosive injury in different time point (1 hour, 24 hours, 72 hours, 7 days, 14 days after damage). The pathological characteristics of corelative tissues were examined in detail under light and electron microscopes. The content of LPO, SOD, NO in soft tissue, brain, blood, and cerebral water content were measured, compared with control. The distribution and tissue location of NOS were studied by histochemistical and immunohistochemistic methods. Their relationship was studied to assess the pathological and physiological characteristics of maxillofacial injuries and brain damage associated.

撞击伤各组动物于伤后6h、爆炸伤各组动物于伤后不同时期（1h、24h、72h、7d、14d）开颅检查颅脑损伤情况，切取伤区肌组织、左侧脑组织、脑干标本行光镜、电镜观察；测定伤后肌组织、脑组织与血中LPO、SOD、NO含量及脑水含量变化；并使用组织化学和免疫组织化学的方法，观察伤后脑组织中NOS的分布和组织定位，研究伤后机体的病理及病理生理学改变特点，以及颅脑损伤与机体自由基反应、NO含量改变之间的关系，证实其病理作用。

The radioactivity immune analysis was adopted to determine serum extradio-E2 (E2), progesterone and prolaction in Crab-eating Macaque after copulation.

方法］采用放射免疫分析法测定食蟹猴配种后血清雌二醇(E2)、孕酮和催乳素的水平变化。

Methods: Four hantavirus strains, which were positive by antigen immunofluorescence assay, were analyzed by reverse transcription polymerase chain reaction, nucleotide sequence and cell culture.

采用逆转录－聚合酶链反应、核苷酸序列测定和细胞培养方法对免疫荧光抗原阳性的4份鼠肺标本进行汉坦病毒分析。

When we begin to the medicine, urine protein was measured in urine volume of 24 hour the every two day. 4. Specimen collections and laboratory check: In the 21 days of pregnant rats, infants were taken which registered weight and height after aesthesia. Cr and BUN in the blood were measured.

标本收集和实验室检查：于妊娠第21天在戊巴比妥钠(50mg/kg)麻醉下，剖宫取胎，记录胎仔体重，身长；同时抽取腹腔静脉血，分离血清以待测定血尿素氮，血肌酐；取左肾部分置于10%甲醛液浸泡固定，待用HE染色、PAS染色及免疫组织化学染色；另取部分肾脏剪成体积约1cm〓大小组织块，生理盐水冲洗至发白，-70℃保存。

Methods The serum and synovial fluid of the pathologic knee joints were obtained from patients with RA,and samples from leg traumatic amputees were used as the control group.

方法用酶联免疫吸附法测定27例类风湿关节炎患者血清及病变膝关节滑液中RANTES、MCP-1水平，并与外

Methods The Biotin-Strept -Avidin enzyme linked immunosorbent assay, spectrophoto-metric assay were used to measure the plasma levels of CT-1 and sICAM-1 in 56 patients with hypertension before and after treatment of Perinopril or Benidipine and 30 normal controls.

应用生物素－链霉亲和素酶联免疫吸附法对56例高血压患者应用培哚普利和贝尼地平治疗前后及30名正常健康人，测定血浆CT-1水平和血浆sICAM-1水平。

Methods After Candida albicans were inoculated into the specific pathogen free mice, the total amount of Candidas in cecum and the amount of which attached to the mucosal membrane were counted at different interent intervals. The lymphocyte proliferation in Peyer's patch and in lamina proper was shown by BrdU incorporation, meanwhile B cells isotype switch in PP was investigated. IgA plasma cells were shown by immunohistochemical staining. Specific IgA antibodies to Candida albicans were measured with ELISA.

采用无特殊病原菌动物经口喂入白念菌，在不同时相处死后，观察肠内白念菌总数及肠粘膜表面白念菌粘附数量；取肠系膜淋巴结做白念菌选择培养，观察移位体内发生率；采用Brdu体内掺入显示局部粘膜淋巴细胞的增殖情况；流式细胞计数潘伊尔氏结细胞表面IgA阳性率；免疫组化染色后计数固有层中IgA浆细胞数量变化；ELISA法测定肠粘液中特异抗白念菌IgA含量。

The purpose of the present study was to investigate the role of central arginine vasopressin in corticotropin releasing hormone-induced fever in the rat. Guide cannulae were inserted into the third ventricle and placed over the ventral septal area. The content of arginine vasopressin in the VSA of the brain was determined by radioimmunoassay. Colon temperature was monitored in lightly restrained rats by insertion of a catheter-mounted thermistor probe 5 cm in the rectum.

实验对大鼠进行第三脑室和脑腹中隔区插管，用数字体温计测量大鼠的结肠温度，用放射免疫分析法测定脑中隔区精氨酸加压素(arginine vasopressin， AVP)含量，观察脑中隔区AVP在大鼠促肾上腺皮质激素释放激素(corticotrophin releasing hormone， CRH)性发热机制中的作用。

Methods Radio-immunological method and radioligand combination were used to determine the contents of plasma cortisol and the GR contents of blood corpuscles of 25 MS cases.

方法用放射免疫法和放射配体结合法测定25例MS患者的血浆皮质醇含量和血白细胞GR含量。

The roots and leaves of the four reed ecotypes were determined for thelevels of abscisic acid and cytokinins using an enzyme linked immunosorbent assay throughout the whole of growing season.

采用酶联免疫吸附法，测定了整个生长季节四种芦苇生态型根和叶组织中脱落酸和细胞分裂素含量的变化。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。