免疫测定

The mechanical contraction of circle muscularis strips, the contractile response of dispersed smooth muscle cells, the change of 〓 stimulated by sennoside with different concentration and that stimulated by Ach after incubated with sennoside were evaluated. The translocation of RhoA by sennoside were measured by laser confocal scanting microscopy too.

检测不同浓度的番泻甙对小肠环行肌条和游离环行平滑肌细胞的收缩作用及对〓的影响，然后测定番泻甙对Ach刺激的小肠环行肌条、游离环行平滑肌细胞收缩反应和细胞内〓变化的影响作用，并通过免疫荧光实验检测番泻甙对RhoA的活化和空间转位的影响。

The expression of PCNA tested by the endometrium sectioning method was compared with the two methods mentioned above.

结论经内膜吸管获取宫腔收集液，用涂片免疫组化法及FCM测定子宫内膜脱落细胞的PCNA，均可反映性激素对子宫内膜增殖状态的影响，在HRT时子宫内膜监测中是可行的。

Objective To estimate the value of gold immunochromatography assay of serum heart-type fatty acid-binding protein for diagnosis of AMI.

目的探讨国内研制的胶体金免疫层析法试剂测定血清心肌型脂肪酸结合蛋白及其在急性心肌梗死诊断中的应用价值。

Objective:To establish a real-time quantitative reverse transcription polymerase chain reaction method for examining the expression of sialic acid-binding immunoglobulin-like lectin-one (Siglec-1)gene in the peripheral blood mononuclear cells in patients with coronary heart disease,so as to explore the relationship between Siglec-1 mRNA expression and the development/progression of CHD.

目的：建立检测人Siglec-1(sialic acid-binding immunoglobulin-like lectins，唾液酸结合的免疫球蛋白样凝集素1，CD169)mRNA 含量的实时荧光定量RT-PCR 的方法，并用来测定冠心病患者外周血单个核细胞中Siglec-1的基因表达水平，探讨Siglec-1基因表达水平与冠状动脉粥样硬化发生发展的关系。

The affinity constant of E1-McAb reacting with coated complete antigen was 8.2×10^8L/mol. Biotin-streptavidin complex was used for the determination of E1. The calibration curve of free E1 covered a range of 10～10 000 pg/mL.

以生物素化的羊抗鼠免疫球蛋白及辣根过氧化物酶标记的链亲和素为标记体系，通过竞争抑制的方式测定游离的雌酮，结果表明：雌酮在10～10000pg/mL内呈线性关系。

In this study, the content of UCP1 in BAT in Tree Shrews was measured during cold exposure for 0 day, 7 days, 21 days and 28 days by enzyme-linked immunosorbent assay.

自备抗血清采用酶联免疫法测定了中缅树鼩在(5±1)℃冷暴露0d、7d、14d、21d、28d时，褐色脂肪组织中解偶联蛋白1的含量。

At the end of experiments, the rats were decapitated for sampling blood, pituitary gland and hypothalamus tissues, followed by assaying β-EP concentrations with radioimmunoassay.

实验结束采集动物血浆、脑垂体及下丘脑组织，用放射免疫技术测定β-EP含量的变化。

Methods Fifty white guinea pigs with normal hearing were randomly divided into the control group, ligustrazine group and traditional Chinese drug group. The alternations of hearing function were evaluated by the examination of auditory brainstem response. Some specimens were perfused by AgNO3 and observed the damage of bristle cell. And some were sent for immunohistochemistry to detect the expression of iNOS. Results Compared with the control group, the threshold of ABR in ligustrazine group and TCD group were tignificantly different.

将50只健康白色红目豚鼠随机分为对照组、顺铂组和中药组，各组动物均于用药前及停药后测试听觉脑干诱发电位，采用耳蜗铺片观察毛细胞损伤情况，应用免疫组织化学方法测定各组动物耳蜗内iNOS的表达情况。

Establishing the atherosclerotic model of rabbits by feeding them with cholesterol, we measured their body weight, TC, TG and LDL. Drawing the materials from rabbit aortic wall and embedding them with paraffin to make into section preparation, we detected type Ⅰ collagen, NF-κB, VCAM-1, CD36, type Ⅰ collagen mRNA and NF-κB mRNA with immunohistochemistry and in situ hybridization technique and by means of color image analyzer .

通过高脂饲料建立兔AS模型，测定体重及血脂成分的改变；取其主动脉壁，石蜡包埋制成切片，采用HE、免疫组化及原位杂交法染色，光镜观察AS病变，并应用CMIAS真彩色医学图像分析系统，检测Ⅰ型胶原mRNA及其蛋白、NF-κBmRNA及其蛋白、VCAM-1及CD36蛋白表达定量A值，与对照组进行比较。

Plasma PCT and IL-18 levels in 98 patients with liver cirrhosis and 30 normal control were determined by immunoluminometric assay and ELISA, respectively.

采用免疫发光法和ELISA法测定 98例肝硬化患者和 30例正常对照者血浆PCT、IL - 18水平。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。