免疫测定

The molecular approach to detecting peptide hormones using cDNA pro bes should also be much faster than the immunological method because i t can take years of tedious purifications to isolate peptide hormon es and then develop antiserums to them.

采用cDNA探子来测定肽激素的这一分子生物学方法同时也应该比免疫学的方法速度来得快，因为对于免疫的方法来说，需耗费好几年枯燥的提纯进程，方能将肽素分离了出来，然后再培养出针对它们的抗血清。

The molecular approach to detecting peptide hormones using cDNA probes should also be much faster than the immunological method because it can take years of tedious purifications to isolate peptide hormones and then develop antiserums to them.

采用cDNA探子来测定肽激素的这一分子生物学方法同时也应该比免疫学的方法速度来得快，因为对于免疫的方法来说，需耗费好几年枯燥的提纯进程，方能将肽素分离了出来，然后再培养出针对它们的抗血难句类型：复杂修饰、易混指代

Analysis of endohormone using ELISA(Enzyme Linked Immunological Sorbent Assay)showed contents of ABA increased while iPA decreased during bulbing,which is similar tophysiological dormany and sencence.

采用酶联免疫吸附测定法对试管鳞茎形成过程中的内源激素分析结果表明，鳞茎形成过程中，内源激素变化表现休眠和衰老的生理趋势，ABA含量不断升高，iPA含量不断下降。

Grade Ⅳ is that the rat circumrotates to right in automatic action.②15 rats in each group were selected. 24 hours, 48 hours, one week after operation, we opened the skulls, took out the brain and used 2,3,5-Triphenyltetrazoluim Chloride staining to measure infarction volume, hematoxylin-eosin staining to observe the pathological change , and immunohistochemistry to detect the infiltration of CD34+ cells.

将两组大鼠各取15只，分别于术后24，48 h和1周开颅取脑，用2，3，5-氯化三苯基四氮唑染色测定脑梗死体积、苏木精-伊红染色检查脑组织病理变化、免疫组织化学检测CD34阳性细胞浸润情况。

Objective To explore the association between the level of folate,VitB12 and acute cerebral infarction disease.Methods 88 acute ischemic cerebrovascular stroke disease events were disparted in two subgroups,32 of arteriosclerotic cerebral infarction,56 of lacunar cerebral infarction,and 35 contrast group were set up.All the participants' serum folate,VitB12 were measured when they entered the hospital.

目的 探讨急性脑梗死患者血清叶酸、VitB12水平及其临床意义方法对88例首次发病的急性缺血性脑中风患者分两亚组，动脉硬化性脑梗死组32例，腔隙性脑梗死组56例，并设对照组35例，入院后用罗氏公司Elecsys 2010型全自动电化学发光免疫分析仪测定血清叶酸、VitB12浓度。

Methods The bile duct of rabbit was excided and a pipe supporting bile duct was inserted through duodenal major papilla to establish a model. V.G staining and TGF-beta 1 immunohistochemistry staining on tubal wall of rabbit bile duct in different times were used to determine quantitatively the intensity of light TGF-beta 1 positive area and the relationship between the integral-optical density of bile duct tissue collagen content and time.

切开兔胆管并自十二指肠乳头行胆管内支撑方法建立模型，将不同时期梗阻段胆管壁进行V.G染色和β1型转化生长因子（transforming growth factor beta 1，TGF-β1）免疫组化染色，测定TGF-β1阳性区域的光度以及胆管组织胶原含量积分光密度随时间变化关系。

Methods Human luteinized granulosa cells were isolated from follicular fluid obtained during oocyte retrieval of in vitro fertilization-embryo transfer program and were cultured with M199 medium plus various concentration of leptin (0, 10, 30, 100, 300 ng/ml),human menopausal gonadotropin (hMG, 0, 0.1, 0.2, 0.5, 1, 2, 5, 10 IU/ml) and testosterone 100 μg/ml. For 2 days the media were collected for estradiol and progesterone measurements.

方法将来自体外受精-胚胎移植的卵巢黄素化颗粒细胞纯化后，在不同浓度瘦素(0、10、30、100、300 ng/ml)和人绝经期促性腺激素（hMG，0、0.1、0.2、0.5、1、2、5、10 IU/ml）单独或联合作用下进行体外培养，收集培养液，采用放射免疫方法测定颗粒细胞产生雌二醇及孕酮的量。

Methods Human luteinized granulosa cells were isolated from follicular fluid obtained during oocyte retrieval of IVF-ET program and were cultured with M199 media plus various concentration of follicle stimulating hormone (FSH, 0% 0.5% 5% 10% 25% 50) or human chorionic gonadotropin(hCG, 0% 5% 10% 50% 75% 100IU/ml). The media were collected and PAPP-A in the media were measured by time-resolved fluoroimmunoassay.

将来自体外受精—胚胎移植的卵泡黄素化颗粒细胞分离纯化后，利用不同剂量促卵泡激素(FSH，0、0.5、5、10、25、50)或人绒毛膜促性腺激素(hCG，0、5、10、50、75、100IU／ml)刺激体外培养的颗粒细胞，收集培养液，采用时间荧光免疫分析法测定培养液中PAPP-A的量。

Methods: The immunohistochemical expression of IGFBP-2 protein was measured by image analysis in 35 surgically resected specimens of gastric cancer (include normal mucosae, primary tumors and metastases of lymph node).

对35例外科切除胃癌标本的正常粘膜、肿瘤原发灶和淋巴转移灶进行IGFBP-2蛋白的免疫组化染色，并用计算机图象分析软件测定其积分光密度。

Serum total cholesterol,triglycerideand ICAM-1 were quantitated before and 2,4,6,8 weeks after the treatment.2,4,6 and 8 weeks after treatment,6 animals of the experiment group and 2 of the control group were randomly chosen and sacrificed.

各组于处理的第0周和第2、4、6、8周抽血测定血清ICAM-1、总胆固醇、甘油三酯水平，并于第2、4、6、8周分批处死动物，行双侧股骨头病理切片和髋周软组织ICAM-1免疫组化检测，并对股骨头行常规病理组织学观察。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。