免疫测定

Methods The expressions of TNF-α and TGF-β1 proteins in uteroplacental tissues were detected with immunohistochemistry in 28 early spontaneous aborters and 28 normal early pregnant women.

采用免疫组织化学技术半定量测定28例早期自然流产患者和28例正常妊娠妇女绒毛和蜕膜组织内TNF-α及TGF-β1表达强度。

Morphology of the EPCs was observed, and the growth curve of the EPCs was investigated. Surface antigens of the EPCs were analyzed by the flow-cytometer. The capability of intaking the acetylated low-density lipoprotein of the EPCs was detected using fluoresencent chemical method. The vasoformative capability and genetic stability of EPCs were cultured in matrigel, and examined by karyotype analysis.

观察培养细胞的形态，测定其生长动力学，采用流式细胞仪及免疫细胞化学方法检测其表型，应用细胞荧光化学法分析EPCs对乙酰化低密度脂蛋白(acetylated low-density lipoprotein， ac-LDL)的摄取功能，Matrigel上培养并检测其形成血管能力，染色体核型分析其遗传稳定性，裸鼠实验、软琼脂实验观察致瘤性。

All the cases were phlebotomized venously 8ml under fasting condition in the morning.Serum No and IL-6 were determined by colorimetry and radioimmunoassay method respectively.

血清NO采用硝酸还原酶法检测，血清IL-6 采用放免法检测，血浆D-二聚体和GMP-140采用酶联免疫吸附双抗体夹心法原理定量测定。

All the cases were phlebotomized venously 8ml under fasting condition in the moming.Serum No and IL-6 were determined by colorimetry and radioimmunoassay method respectively.

血清NO采用硝酸还原酶法检测，血清IL-6采用放免法检测，血浆D-二聚体和GMP-140采用酶联免疫吸附双抗体夹心法原理定量测定。

Results TNFα-induced ICAM-1 expression in HK-2 cells was increased in both protein and mNRA levels( P .01). NFkB inhibitor N-tosyl phenylalanine chlormethyl ketonecould inhibit these effects of TNFα.

方法用 HK-2 细胞作靶细胞，用细胞酶联免疫吸附法和 Northem 杂交观察 ICAM-1 的蛋白和基因的表达，以电泳迁移率变动法测定转录因子核因子 kB和激活蛋白 1(AP-1)的活性。

The molecular approach to detecting peptide hormones using cDNA probes should also be much faster than the immunological method because it can take years of tedious purifications to isolate peptide hormones and then develop antiserums to them.

采用 cDNA 探子来测定肽激素的这一分子生物学方法同时也应该比免疫学的方法速度来得快，因为对于免疫的方法来说，需耗费好几年枯燥的提纯进程，方能将肽素分离了 59。

The molecular approach to detecting peptide hormones using cDNA probes should also be much faster than the immunological method because it can take years of tedious purifications to isolate peptide hormones and then develop antiserums to them.

采用cDNA探子来测定肽激素的这一分子生物学方法同时也应该比免疫学的方法速度来得快，由于对于免疫的方法来说，需耗费好几年枯燥的提纯进程，方能将肽素分离了出来，然后再培养出针对它们的抗血清。

The molecular approach to detecting peptide hormones using cDNA probes should also be much faster than the i******unological method because it can take years of tedious purifications to isolate peptide hormones and then develop antiserums to them.

采用cDNA探子来测定肽激素的这一分子生物学方法同时也应该比免疫学的方法速度来得快，因为对于免疫的方法来说，需耗费好几年枯燥的提纯进程，方能将肽素分离了出来，然后再培养出针对它们的抗血清。

The molecular approach to detecting peptide hormones using cDNA probes should also be much faster than the immunological method because it can purifications take years of tedious to isolate peptide hormones and then develop antiserums to them.

采用 cDNA 探子来测定肽激素的这一分子生物学方法同时也应该比免疫学的方法速度来得快，因为对于免疫的方法来说，需耗费好几年枯燥的提纯进程，方能将肽素分离了出来，然后再培养出针对它们的抗血清。

The molecular approach to detecting peptide hormones using cDNA probes should also be much faster than the immunological method because it can take years of tedious purifications to isolate peptide hormones and then develop antiserums to them.

采用cDNA探子来测定肽激素的这一分子生物学方法同时也应该比免疫学的方法速度来得快，因为对于免疫的方法来说，需耗费好几年枯燥的提纯进程，方能将肽素分离了出来，然后再培养出针对它们的抗血清。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。