免疫测定

Meanwhile we observed the change of symptoms, biopsy, T cell inferior group of serum (CD3、 CD4、CD8)、immunoglobulin (IgG、IgA、IgM) and the alexin (C3、C4), cAMP、 cGMP. CD3、CD4、CD8 are detected by immunomomoclonal antibodies APPAP; IgG、IgA、IgM are tested by immunoturbidimetry assay; the content of cAMP and cGMP in blood plasm are tested by radioimmunoassay.

所有观察病例于治疗前均作血、尿、大便常规化验；心、肝、肾功能检查；阴道分泌物检查包括念珠菌、滴虫、阴道清洁度；尿糖、血糖检查；分别观察中药组和西药组治疗前后相关症状及体征变化情况，活体组织病理检查的变化情况；血清T淋巴细胞亚群（CD3、CD4、CD8）和免疫球蛋白(IgG、IgA、IgM)以及补体（C3、C4）各项指标的变化情况；血中cAMP、cGMP含量变化情况，T淋巴细胞亚群水平测定采用单克隆抗体APAAP桥联酶标技术，血清免疫球蛋白IgG、IgA、IgM及补体C3、C4的测定采用免疫透射比浊法。

After hybridization of two DNA probes with target DNA, EDTA-〓 and β-diketone come close to each other, and an EDTA-〓-β-diketonate ternary complex having strong and long-lived fluorescence was formed, thus the target DNA was detected sensitively with a detection limit of 6 pM (0.6 fmol per assay) by time-resolved mode.

系统研究了使用EDTA-〓为标记物的石墨炉原子吸收光谱免疫分析法，并将之用于人血清中AFP和水中BSM的测定。该法中使用了EDTA-〓标记SA及生物素标记抗体或抗原，在96孔板中的免疫反应结束后，利用稀硝酸将反应产物分解，然后用石墨炉原子吸收光谱法对免疫反应产物进行测定。

The level of tumor necrosis factore alpha, interleukin-6 (IL-6) and interleukin-8 (IL-8) were evaluated using ELISA method. Western blotting was used to detect the protein expression of protein kinase B and lipid phosphatase PTEN. Immunoprecipitations and radioactivity of γ〓P incorporated into its specific substrate histon H〓B was utilized to determine the PKB activation. Phosphate concentration released from PTEN-specific substrat diC16PIP3 was assessed using green reagent method.

以细胞计数、噻唑蓝比色法测定细胞增殖能力，酶联免疫法检测培养上清液中细胞因子TNFα、IL-6、IL-8水平，Western免疫印记定量蛋白激酶B、蛋白磷酸酶PTEN表达水平，免疫沉淀、特异底物组蛋白H2Bγ〓P掺入量测定PKB活性，特异底物dic16PIP3脱磷酸绿色试剂法反应PTEN活性。

The result indicated that the percentage of lymphocyte were decreased, the percentage of neutrophile granulocytes were magnified significantly with the lighting time increasing, which was due to the level of the melatonin inhibited in the long time lighting; and to supply different exogenous dose, induce the percentage of lymphocyte were magnifying, the percentage of neutrophile granulocytes were decreased. And the statistical analysis showed there was significant difference.

结果表明：在给予不同光照时间的条件下，随着光照时间的延长，褪黑素的分泌水平受到抑制，导致具有特异免疫功能的淋巴细胞百分率下降，具有非特异免疫功能的中性粒细胞百分率上升，经统计分析表明不同组间的测定结果均存在显着差异；采用24h全天光照，用以模拟机体切除松果腺的情况，再给予一定剂量外源性褪黑素，导致机体内具有特异免疫功能的淋巴细胞百分率上升，具有非特异免疫功能的中性粒细胞百分率下降，经统计分析表明不同组间的测定结果均存在显著差异，并且高剂量褪黑素对机体的影响更为明显。

Serum level of TNF-α was measured by unsaturation assay; Serum level of IL-2、IL-6、 IL-10 were measured by radioimmunoassay.

采用非饱和法测定肿瘤坏死因子α的水平；采用放射免疫法测定白介素-2(IL-2)、白介素-6(IL-6)和白介素-10(IL-10)的水平；采用酶联免疫吸附法测定肌钙蛋白Ⅰ的水平；采用速率法测定丙氨酸转换酶的水平；采用肌氨酸氧化酶法测定肌酐的水平。

Serum alt,ast,and bile acid were observed by enzyme assay and the ratio of ast/alt was worked out. serum bilirubin was measured by peariman and lee method. serum albumin was tested by bromphenol assay and was simultaneously measured to hyaluronic acid, type ⅲ procollagen, laminin and type ⅳ collagen by ria. meanwhile, prothrombin time and platelet count were measured and so on. results: the level of serum aca was raised increasingly in different cirrhotic cases with different classifications of child-pugh, according to severity of the condition.

应用酶联免疫吸附法分别对不同分级的肝硬化患者血清中抗心磷脂抗体的含量进行了测定；用酶法测定了alt、ast与胆汁酸并计算ast/alt值；用peariman和lee改良法测定血清胆红素；用溴甲酚氯比色法测定血清白蛋白；用放射免疫分析法测定透明质酸、ⅲ型前胶原氮端肽、层粘连蛋白与ⅳ型胶原；并测定了凝血酶原时间与血小板计数等相关指标。

Methods 1. The mice were divided into control and 3 Ganodermalucidum spores oil groups (at low, moderate and high doses), orally given forconsecutive 30 days. The body, spleen, thymus weight were observed, study on theeffect of the drug on the cellular immune function by proliferation and transformationof spleen lymphocytes test; study on the effect of the drug on the humoral immunefunction of mice by serum erythrocytolysin test; study on the effect of the drug on themononuclear macrophage function by carbon clearance and neutral red test.

实验方法 1设玉米油对照组和低、中、高三个灵芝孢子油剂量组，连续灌胃30天后观察小鼠体重、胸腺、脾脏重量指数；采用吞噬中性红和碳廓清实验测定对小鼠单核-巨噬细胞功能的影响；血清溶血素实验测定对小鼠体液免疫功能的影响；ConA诱导脾淋巴细胞转化实验测定对小鼠细胞免疫功能的影响；MTT法测定对小鼠NK细胞杀伤活性、脾细胞产生TNF-α和IL-2含量的影响。

After 24 hours, all rats were anesthetized again and their left common carotid arteries were cannulated to facilitate blood withdrawal for serum sample. The contents of tumor necrosis factor-a and interleukin-6 (IL-6) in serum were determined before and after experiment by ELISA; the activity of superoxide dismutase and content of malondialdehyde were determined by xanthinoxidanse method and modified TBA microdetermination. The content of NO2(superscript -)/NO3(superscript -), and activity of nitric oxide synthase and inducible NOS were determined by nitrate reductase method, and chemical chromogenic reaction, respectively; the immunoglobulin G, IgA, IgM and complement 3 (C3) and C4 were determined by immunoprecipitation method before and after experiment.

实验评估：手术创伤后24h进行采用酶联免疫吸附法测定实验前后血清肿瘤坏死因子α、白细胞介素6含量；应用黄嘌呤氧化酶法测定超氧化物歧化酶活性、改良TBA微量法测定丙二醛水平；以硝酸还原酶法、化学显色法分别检测NO2/NO3浓度及一氧化氮合酶、诱导型一氧化氮合酶活胜；以免疫比浊法经全自动生化分析仪检测血清免疫球蛋白IgG、IgM、IgA及补体C3、C4含量。

Western blots were performed to investigate the cross-reactivity of various labeled antibodies with serum proteins from other fish species. Results Immunoglobulins of five fish species were purified, including bighead carp, carp, argus snakehead fish, ricefield eel, sea bass. The titers of five rabbit anti-sera obtained up to 1:32 examined by agarose double immunodiffusion. Five kinds of purified rabbit anti-fish immunoglobulins antibodies were labeled with HRP and their activities were up to 1:10000 examined by EUSA. Four kinds of rabbit anti-fish immunoglobulins antibodies labeled with HRP could react with serum proteins from other fish species.

结果 纯化了鳙、鲤、乌鳢、黄鳝、鲈五种鱼血清免疫球蛋白，免疫双扩散法测定兔抗这五种鱼免疫球蛋白的抗血清效价均达到1：32，并对纯化的兔抗鳙、鲤、乌鳢、黄鳝、鲈五种鱼类免疫球蛋白的抗体进行了HRP标记，ELISA测定标记抗体的效价达到1：10000左右，Western blots显示标记抗体与部分其他鱼类免疫球蛋白之间存在不同程度的交叉反应。

The method of enzyme-linked immunosorbant assay was employed to determine the contents of indoleacetic acid, abscisic acid, gibberellin (GA3) and Zeatin under pot culture.

在盆栽基质培养条件下，进行了打顶和打顶+生长调节剂处理，采用酶联吸附免疫测定技术分析了烤烟根和叶中内源IAA、ABA、GA3和ZR的变化。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。