免疫测定

Rats were sacrificed at 0, 6, 12, 24 and 48 hours after reproduction of pancreatitis in both groups. The intestinal mucosal permeability was assessed with 99mTechnetium Diethlene Triamine Pentacetic Acid method, and the expression of substance P was evaluated with reverse transcriptase polymerase chain reaction and Western blotting analysis.

分别于制模成功后0、6、12、24和48 h处死两组大鼠，并留取标本；利用同位素法测定肠黏膜通透性，分别用逆转录-聚合酶链反应和蛋白质免疫印迹法检测P物质在肠组织中的mRNA和蛋白表达水平。

Group of malignant tumor and normal control were chosen and the level of serum Lipoprotein investigated by Turbidimetric Immunoassay.

方法应用免疫透射比浊法测定恶性肿瘤组和正常对照组血清Lp水平。

Methods By the use of immune turbidimetric method, CRP level in different kinds of coronary heart diseases and effects of medicines on CRP were investigated.

采用免疫比浊法测定不同类型冠心病CRP的浓度及观察用药后CRP的变化。

Serum hs-CRP in 253 patients with malignant tumor, 99 patients with benign tumor, 92 patients with inflammatory mass and 200 healthy people were evaluated by turbidimetric method based on the peak rate principle.

应用免疫速率散射比浊法测定治疗前的253例恶性种瘤、99例良性肿瘤、92例炎性包块患者及200例健康人血清hs-CRP水平。

Test the serum levels of hs-CRP of every group by turbidimetric immunoassay,and analyse the difference between the serum levels of hs-CRP before and after the treatment.

采用透射免疫比浊法，分别测定各组血清中hs-CRP的浓度，并分析治疗前后hs-CRP水平的差异。

Methods: The Serum cystatin-C concentration of 66 patients with cirrhosis, 64 patients with cancer and 62 healthy subjects were determined by turbidimetric immunoassay method.

应用德灵公司全自动特定蛋白分析仪采用免疫散射比浊法测定66例肝硬化、64例肝癌与62例非肝肾病的胱抑素C。

The method of turbidimetric immunoassay can be applied on automatic analyzer for detection of RBP in the serum.

结论免疫透射比浊法测定血清视黄醇结合蛋白，方法简便、快速、灵敏，结果准确，可用自动分析仪测试，适合临床检验科应用。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

We used the immunohistochemical ABC method to detect the expression of NF-κB at 2、 6、 12、 24、 48、 72 hours after infected by Salmonella typhimurium, and we deal the data statistically with univariant analysis of variance and Student-Newman-Keuls multiplerange test.

三组分别在感染鼠伤寒沙门氏菌后2、6、12、24、48、72小时6个时点，用免疫组织化学方法(抗NF-κB p65抗体、ABC法，石蜡包埋)和阳性细胞面积分布率半定量测定小肠、结肠及肾脏中NF-κB活性表达百分率，采用单变量多因素方差分析和Q检验做统计学处理。

Akt,AMPK and ERK proteins were detected by western blot analysis,and the distribution of GLUT4 in these skeletal muscles was observed by immunofluorescence. Results The expression of GLUT4 on the cell membrane was significantly higher in the electrically stimulated groups than in the unstimulated groups.

用Western Blot法测定骨骼肌中蛋白激酶B(protein kinase B，PKB/Akt)、腺苷酸激活的蛋白激酶、细胞外信号调节激酶的表达和活性变化，用免疫荧光方法观察GLUT4在细胞膜及细胞内膜的分布。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。