免疫测定

Methods Serumal CA153,CEA,AFP and CA125level of patients with malignant and benign mammary neoplasm was investigated respectively by electrochemoluˉminescence immunoassay.

采用电化学发光免疫分析法测定乳腺癌患者及乳腺良性疾病患者血清的CA153、CEA、AFP和CA125。

Results After screening, 59 subtracted library clones were isolated which were specific for strain VIB72, and the DNA sequences of these clones were determined. Seventeen fragments showed high homology to the genes of known functions in other bacteria. This includes soluble lytic murein transglycosylase, mobilization protein, transposase (IS66), resistance-related protein (metallo-beta-lactamase and acetyltransferase family), toxin protein (DT-201 and alveicin A immunity protein), ATP-dependent endonuclease of OLD family like protein, SocE and GTP-binding protein HflX (high frequency of lysogenization).

通过对差减文库筛选，分离到59个对菌株VIB72的克隆，并对这些克隆的DNA序列进行了测定。17个基因片断与其它细菌的已知功能的基因有较高的同源性，其中包括可溶性溶胞壁质转糖基酶、转移蛋白MobA和MobC、转座子IS66、抑制相关蛋白（金属β-内酰胺酶和乙酰转移酶家族）、毒素蛋白（DT-201和alveicin A免疫蛋白）、与OLD 家族相似的ATP依赖性核酸内切酶以及SocE 和GTP结合蛋白HflX。

The inducible nitric oxide synthase is obviously expressed in speen of rats under the predator stress, suggesting important function for iNOS in speen physiology. However, the expression and subcellular localization for NOS in speen development and differentiation are largely unknown. In the present study, the changes in iNOS activity in spleen of rats under predator stress were studied to explore the role of iNOS in the decreased immunoactivity of the stressed rats. The changes in iNOS were observed with immunobistochemical method, and the contents of NOS and NO in spleen with biochemical method.

在捕食压力下，大鼠脾脏诱导型一氧化氮合酶明显表达，说明iNOS起到了重要的生理功能，然而，NOS的表达及在亚细胞水平的定位以及相应的差异变化等都很不明确，本试验采用捕食应激模型，利用免疫组织化学方法和生化测定，检测心理性应激对大鼠脾脏iNOS活性的影响及探讨这种影响与应激动物免疫力下降之间的关系。

Changes of the NOS activity and the expression of NOS protein in central nervous systems of rats,which were subacutely intoxicated with oral exposure to lead acetate for 30 days,were observed with improved NADPH techniques and immunohistochemistry to study neurotoxic mechanism of lead.

本文采用SD成年大白鼠饮用醋酸铅水染毒30天，然后用改进的NADPH测定法及免疫组化SP法观察脑组织中NOS酶活性及NOS酶蛋白亚型的变化。

Methods The model of subacutely aging rats were made by injecting Dgal into the rats, abdominal cavity continually. After lavaging HSWY, the apoptosis rate and the levels of Fas and FasL mRNA were measured by TUNEL and RTPCR; the expressions of Fas/Fasl protein were detected by SP immunocytochemistry.

采用D半乳糖连续腹腔注射致亚急性衰老大鼠模型，同时以何首乌饮灌胃作为延缓衰老组；造模后应用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法检测各组大鼠卵巢细胞凋亡情况；测定各组Fas和FasL的信使核糖核酸水平；SP免疫组化法检测卵巢细胞Fas和FasL蛋白的表达情况。

With computer image analysis system, the expressions of NF-κB and ICAM-1 and subepithelial collagen deposition were checked.

将40只大鼠随机分为对照组、哮喘组（以卵蛋白激发的大鼠哮喘模型）、布地耐德早期治疗组、布地耐德晚期治疗组，应用免疫组化技术结合计算机病理图像分析系统测定大鼠气道上皮胶原的沉积和气道上皮细胞NF-κB、ICAM-1的相对含量。

To observe the change of behavior index and mensurate the contents of plasma CORT, CRH and ACTH by using radiation immunity methods, and observed the change of immunity function by testing IL-2 and lymphocyte multiplication produced by spleen cell. At the same time, the author examined the change of thalamencephalon CRH mRNA and pituitary ACTH mRNA.

观察行为学指标的变化，利用放免法测定血浆皮质酮、下丘脑促肾上腺皮质激素释放激素和血浆促肾上腺皮质激素的含量，并通过脾细胞产生白细胞介素2（IL-2）水平和淋巴细胞增殖试验观察大鼠免疫功能的变化，同时检测大鼠下丘脑CRH mRNA和垂体ACTHmRNA含量变化。

The concentrations of serum thyrotropin in 48 normals, 20 hatients with hyper thyroidism,10 patients with hypothyroidism and 121 inhabitants in the endemic goiter area were measured by monoclonal-antibody two-side solid-phase EIA and immunoradiometric assay.

应用单克隆抗体双面固相酶联分析法和免疫放射分析法测定48例正常人，20例甲亢病人、10例甲低病人和121例地方性甲状腺肿流行区居民的血清TSH.TSH—EIA法的结果平均值在各组中均低于IR MA法的结果。

Methods Rats aging 4 weeks were immobilized by limb-tail fixation for 2 and 4 weeks respectively, and then the wet weight, gray weight, bone mineral density of femurs and tibiae were measured, the histopathological and histomorphological parameters also were detected, which were used to evaluate the effect of immobilization on bone content and bone structure of femurs and tibiae in growing rats. The VDR expression of femurs and tibiae in the growing rats was evaluated by immunohistochemistry and image analysis.

通过腿－尾固定法对4周龄幼鼠分别进行右后肢2周和4周制动，采用股骨湿重、灰重、骨密度测量，骨组织病理学和骨组织形态学参数测定等方法对比评价制动对幼鼠制动侧股骨和胫骨的骨量、骨结构的影响，采用免疫组织化学和图象分析方法检测制动对幼鼠骨VDR表达的影响。

Sp were evaluated by computer-assisted image analysis software, and the content of OPG determined by immunohistochemistry, which included mean optical density 、positive area density in bone trabecula and total positive area of cancellous bone, were evaluated by computer-assisted image analysis and semiquantitative analysis.

Sp，免疫组化方法结合图像分析半定量分析OPG在胫骨的表达，测定平均光密度、OPG在骨小梁中阳性表达的面积密度和OPG在松质骨骨组织阳性表达的总面积。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。