免疫测定

Methods: Eotaxin protein expression was measured by immunohistochemical techniques in 5 nasal polypous tissue specimens following 30 mg predisone oral doses per day for 7 days, 5 nasal polypous tissue specimens following 10 mg dexamethasone intravenous drops, 5 without any treatment and compared with the results from the inferior turbinate tissue specimens.

随机将15例鼻息肉患者均分为强的松治疗组、地塞米松治疗组和未治疗组，以同期手术的5例鼻中隔偏曲者的下鼻甲黏膜组织作对照，用免疫组化法测定鼻息肉组织中嗜酸细胞趋化因子蛋白的表达。

The non-specific immune functions were studied in four populations of Portunus trituberculatus from Haizhou Bay, Dandong, Zhoushan and Laizhou Bay for the immunity enzyme activities in haemolymph, muscle and hepatopancreas tissue of the crabs. Results showed that SOD activities of haemolymph and muscle were not significant different by four populations.

本文测定了海州湾、辽东湾、舟山沿海和莱州湾4个野生群体三疣梭子蟹的血淋巴、肌肉和肝胰腺组织中的抗氧化及其它与非特异性免疫相关的酶活指标，并对这些指标在不同群体间的差异进行了比较。

The suitable concentration of free Ca〓 and the suitable pH for the activity of the tonoplast-bound CDPK was 10μmol/L and 6. 5, respectively. Its active site was provably oriented toward the cytoplasm.

用我们建立的非放射性同位素测定方法和免疫印迹鉴定了液泡膜上存在一种具有钙依赖蛋白激酶活性的膜固有蛋白。

Methods:establishing s180 tumor model,and establishing the model of pyretic syndrome by alcohol. the centrifuged blood was detected pge-2 by the method of radioimmunity,and observed the condition of hot syndrome.to detect il-8 in tumor tissue of s180 mouse be interfered in hot pathogenic factor by method of abc-elisa.

利用腋下种植s180肉瘤细胞株建立肿瘤模型，并用灌胃乙醇的方法建立肿瘤热证模型，利用放射免疫方法检测s180小鼠血清中pge-2的水平，采用双抗体夹心abc-elisa法测定热邪干预s180小鼠肿瘤组织中il-8的含量。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

The rate of abnormity was30.29%.Conclusion The sensitive and accuracy and spe-cialties of the detection in BALP confirmed good by clinic diagnosis of rachitic,superior to other methods and index.

用全血干化学免疫浓缩法对307例新生儿进行骨碱性磷酸酶活性测定，采用多元线性及逐步回归法分析新生儿BALP活性水平与母亲妊娠因素的相关性。

And52examples that between250and300u/L.The rate of abnormity was30.29%.Conclusion The sensitive and accuracy and spe-cialties of the detection in BALP confirmed good by clinic diagnosis of rachitic,superior to other methods and index.

用全血干化学免疫浓缩法对307例新生儿进行骨碱性磷酸酶活性测定，采用多元线性及逐步回归法分析新生儿BALP活性水平与母亲妊娠因素的相关性。

Methods: MUC1/Y extracellular domain was used as a target molecule to biopan Ph. D. 12 phage randem peptide library. Two protocols using affinity gel and cell culture plates respectively were carried out. Positive phage clones were identified by ELISA. ssDNA sequencing was done on 16 positive phage clones to get the amino acid sequences of MUC1/Y-binding peptides. Immunohistochemistry was done to show the capacity and specificity of positive phage clones to bind the tumor cell lines.

以MUC1/Y黏蛋白的胞外段蛋白(MUC1/Yex)为靶分子，用凝胶亲和法和酶联板法分别筛选十二肽噬菌体随机肽库，ELISA鉴定阳性克隆，DNA序列测定后确定MUC1/Yex结合肽的氨基酸序列；免疫组化鉴定阳性噬菌体克隆与正常及肿瘤细胞的结合能力及特异性。

Endogenous hormones, including gibberellin (GA4), indole acetic acid, isopentenyl adenine and abscisic acid, were analyzed during bolting in spring radish (Raphanus sativus L.).

采用酶联免疫吸附法测定了春萝卜抽薹过程中顶端生长点处嫩叶的赤霉素(GA4)、生长素、细胞分裂素和脱落酸等含量的变化情况。

It is less reliable than serologic tests in determining immune status.

其可靠性较测定免疫状态的血清试验为差。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。