免疫测定

Methods The PCO rat models, prepared by pregnancy hormone combine with human chorionic gonadotrophin, randomly divided into four group as control, model, Chinese crude drug and western medicine group; The levels of serum gonadal hormone were detected by radioimmunoassay; The effects of ZYC on ovaries and ovarial follicular development were observed by light microscope; And the expression level of TNF-α was detected with the immunohistochemical staining technique.

采用孕激素联合人绒毛膜促性腺激素造模法制备PCO模型大鼠。随机分为对照组、PCO模型组、PCO模型组+助孕胶囊组、PCO模型组+克罗米酚组，运用放免法测定血清性激素水平的变化；光镜观察卵巢、卵泡发育的影响，免疫组化学法检测卵巢组织肿瘤坏死因子α表达水平。

The CFb in blank control group was added with IMDM containing 10% FBS and those in Ang Ⅱcontrol group with Ang Ⅱ.The CFb in the 3 test groups were induced with Ang II,then added with Tau at dosages of 40,80 and 160 mmol/L respectively. Determine the proliferation of CFb by MTT method, the collagen content by oxyproline kit, the transforming growth factor- TGF-β1 (TGF TGF-β1) content by ELISA, the cell cycle by flow cytometer, the CFb NO content by nitrate reductase method, the iNOS activity by spectrophotometry, and the expression of iNOS protein by IFA.

胰酶消化法分离培养新生大鼠CFb，用Ang Ⅱ诱导促进其增殖，采用MTT法检测细胞增殖；羟脯氨酸试剂盒检测胶原含量；ELISA法测定转化生长因子β1(TGF-β1)蛋白的含量；流式细胞仪检测细胞周期；硝酸还原酶法、分光光度法和免疫荧光法检测CFb NO含量、一氧化氮合酶活性和iNOS蛋白的表达。

Zymography test and immunohistochemical technique were used separately to determine the activity of MMP-2 and MMP-9 of the paraffine sample and the refrigeration sample.

石蜡标本进行免疫组织化学定位检测MMP-2、MMP-9，冷冻标本用Zymography方法进行MMP-2、MMP-9活性测定。

Immunohistochemistry were used to evaluate express of VEGF in periodontium, computer image analysis system were used for semi-quantitative analysis.

应用免疫组织化学方法测定牙周组织中VEGF表达状况，计算机图像分析系统半定量分析。

Methods: Seven male patients with kidney stones were admitted. Abdominal subcutaneous adipose tissue and perirenal adipose tissue were collected from the operating-patients, and were cultivated with different concentrations of curcumin (10 and 100 μg/ml) in vitro.

用体外组织培养法培养手术后离体人脂肪组织，分别予10和100μg/ml姜黄素干预6h和24h，用夹心酶联免疫吸附测定法检测各脂肪组织培养液中APN和IL-6的含量。

Methods: The male wistar rats were infected with Pneumoniae Klebsiella species by the intratracheal peroral route. CAZ was administered at therapeutic doses intraperitoneally for 7 days and several immunologic parameters were measured.

雄性wistar大鼠采用气管内注入感染法制备肺炎克雷伯氏杆菌性肺炎模型，注射治疗剂量CAZ 7 d后测定免疫指标。

Methods The influence of marine algal polysaccharide on immune function of macrophages inγ-ray irradiat-ed mice was inspected through examining phagocytize activity,IL-1and NO levels of macrophages.

通过测定小鼠腹腔巨噬细胞吞噬功能、产生IL-1和NO能力，考察海藻多糖对γ射线照射小鼠巨噬细胞免疫功能的影响。

Methods:Plasmatic neuropeptid Y concentration was determined with radioimmunoassay in 38 Schizophrenia Patients and 20 normal controls.

采用放射免疫法测定38例精神分裂症患者及20例正常对照者的血浆神经肽Y含量。

Methods Plasmatic AVP concentration was determined with radioimmunoassay in 38 Schizophrenic Patients and 22 normal controls.

采用放射免疫法测定38例精神分裂症患者及22例正常对照者的血浆AVP含量。

Methods:plasmatic neuropeptid y concentration was determined with radioimmunoassay in 38 schizophrenia patients and 20 normal controls.results: compared with normal controls, plasmatic neuropeptid y concentration in schizophrenia patients was significant decreased (t=9.49,p.01).there were significant difference in plasmatic neuropeptid y of patients before and after clozapine treatment (t=2.20,p.05).conclusions: the results suggest that there are abnormal metabolism in plasmatic neuropeptid y of schizophrenia patients .

采用放射免疫法测定38例精神分裂症患者及20例正常对照者的血浆神经肽y含量。结果：精神分裂症患者血浆神经肽y含量较对照组低，差异非常显著（t＝9.49，p.01）。精神分裂症患者经氯氮平治疗4周，患者症状好转后，其血浆神经肽 y含量较治疗前显著升高(t=2.20，p.05)。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。