免疫测定

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果：倒置显微镜和透射电镜观察显示，经SAHA处理的细胞增殖速度显著减慢，细胞体积增大，细胞核较小，形状较为规则，核仁数量减少、体积变小，核内常染色质增多而异染色质减少，核质比例减小，细胞质内线粒体数量增多、线粒体嵴发达，高尔基体较为典型，粗糙型内质网增多，呈现出与正常上皮细胞相似的形态变化；MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用，并有明显的剂量依赖和时间依赖关系；免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达；流式细胞仪检测结果显示，SMMC-7721细胞经SAHA处理后，G0/G1期细胞明显增加，S期细胞则明显减少，细胞被阻滞于G0/G1期；RT-PCR检测结果表明，SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加，24h后更为明显。

The Concentrations of Luteinizing hormone and Oestradiol (E_2) in Peripheral plasma of the white Rock hens from 184 to 239 days of age were determined by the radioimmunoassay.

用放射免疫分析法测定了184—239日龄的白洛克母鸡外周血浆黄体生成素和雌二醇(E_2)的含量。

The Contents of progesterone and oestradiol(E_2) in the milk of 50 Chinese Holstein cows were measured within 23 days after delivery and 12 days during oestrus with radioactive immunity.

自放射免疫激素测定技术出现后，国内外在生殖内分泌领域的研究取得了巨大进展[1~6]。

The concentration of progesterone(P4 ),17β-oestradiol (E 2 )and luteining hormonein the peripheral serum of white Xiang pigs was measured by radioimmunoassaythroughout the whole oestrous cycle.

用放射免疫分析法测定了10头白香猪发情周期外周血清孕酮(P4)、17β-雌二醇(E2)和促黄体素含量的变化。

Changes of progeterone level in the peripheral blood plasma and pooled bucket milk during one oestrous cycle were measured by direct RIA in 10 Holstein-Friesian cows.

采用乳汁孕酮直接放射免疫分析法测定了10头黑白花奶牛外周血浆和乳汁孕酮在一个发情周期中的变化。

We collected the urinary samples of one adult male, three pregnant females and one oestrus female.

我们收集了一只雄性黑叶猴不同阶段、三只雌性黑叶猴妊娠期和一只雌性黑叶猴发情期的尿液，用放射免疫分析法分别测定了雄猴尿中的睾酮、雌猴尿中的雌二醇和孕酮浓度，分析了不同阶段、不同状态下各种性腺激素水平的变化，并将其与黑叶猴的繁殖行为相联系，探讨笼养状态下黑叶猴性腺激素水平与繁殖行为的关系。

To measure the estradiol and progesterone in female oestrus dogs by radioimmunological assay.

放射免疫法测定自然发情母犬体内雌二醇和孕酮水平。

Methods Serum sex hormone levels of 21 patients with azoospermia, 48 patients with oligospermia (there were 13 patients with severe oligospermia in them) and 25 healthy men with normal semen as normal control were determined by chemiluminescent immunoassay.

方法采用化学发光免疫分析法测定21例无精症、48例少精症（其中13例为重度少精症）患者与25例精液正常的健康体检男性的血清性激素水平。

The biological features of hBMSCs and cryogentic storaged cells were analyzed.hBMSCs memberane antigens were identified by immunofluorescence staining.The potential osteogenous differentiation of hBMSCs was evaluated by the osteogeneic inducer kit.

倒置光学显微镜下观察细胞形态及生长情况，细胞计数法绘制细胞生长曲线，并分析冻存细胞复苏后的生长特性，免疫荧光染色测定hBMSCs表面标志，成骨诱导试剂盒检测hBMSCs向成骨分化的潜能。

The levels of serum gonadal hormone were detected by radioimmunoassay. The effects of ZYJN on ovaries and ovarial follicular development were observed by light microscope. The expressed level of LIF was detected by using the immunohistochemical staining technique.

随机分为对照组、PCO模型组、PCO模型组＋助孕胶囊组、PCO模型组＋克罗米酚组，采用放免法测定血性激素水平的变化，光镜观察卵巢、卵泡发育的情况，免疫组化法检测卵巢组织LIF的表达水平。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。