免疫测定

The thickness of alveolar wall, the areas of mesenchyma in lung tissue were measured by HE staining. Immunohistochemical evaluation was to detect the expression of TNF-α, TGF-β1, FN protein.

灌药后第7、14、28天分组处死动物，提取肺组织，HE染色行肺泡炎、肺纤维化程度分级及免疫组化测定肺组织TNF-α、TGF-β_1、FN蛋白表达。

The central theme of this thesis is to investigate the expression of lymphocyte surface marker and concentration of serum cytokines in four groups of patients with sepsis : Group 1, patients with Gram positive bacterial infection; Group 2, patients with Gram negative bacterial infection; Group 3, patients with negative cultures but strong evidence of infection; Group 4, patients without clinical and microbiologic evidence of infection as control group.

本研究主要以临床诊断为主再配合血液培养将病人分为四组：第一组是有革兰氏阳性细菌感染的病人；第二组是有革兰氏阴性细菌感染的病人；第三组是没有培养出细菌的感染性病人；第四组是由临床诊断及微生物培养证实为正常对照组。利用单株抗体接上萤光物质再配合流式细胞分析仪来测定淋巴球上各种细胞膜表面分化抗原的变化及利用酵素免疫分析法来探讨血清各种细胞素之变化。

In addition of cytomorphologic identification, immunolhistochemic technique of bone morphogenic protein-monoclonal antibody, alkaline Phosphatase staining and concentration assays, as well as osteoblasts reaction to PTH-cAMP were used, according to the biologic character of osteoblasts.

除了在细胞形态学上对其鉴定外，还根据成骨细胞的生物学特性，首次引用了骨形成蛋白单克隆抗体免疫细胞化学技术，以及碱性磷酸酶染色和含量测定，成骨细胞对甲状旁腺激素-cAMP反应等方法，验证了经酶消化分离培养的兔颅骨细胞具有成骨细胞的功能和特性。

The induced MSCs were found to be positive for desmin and specific myoglobulin of the skeletal muscle.

MSCs免疫组织化学法测定CD44反应阳性，胞质呈棕褐颗粒，核周明显，CD34呈阴性反应；诱导后MSCs结蛋白、骨骼肌特异肌球蛋白均为阳性表达。

Methods: Forty mice were divided into four groups randomly:normal group,model group,ligation group and treatment group.The model was made by the method of partly ligating the recta outside the body.The level of endotoxin in blood plasma was measured by dynamic nephelometric method and the content of TNF-a in blood serum was detected by the method of radioimmunity.(2)After the mice were killed,their left lungs were taken out for bronchi-alveoli fluid, then the content of MDA in BALF was tested by TBA method.(3)The vitality of PAM in BALF was countied on the counting set of erythrocyte,and the blue-dyed cellswere counted, then the percentage of PMN was worked out.(4)The pathological features of the mice\'s lung tissue were observed with routine and ultramicro pathological mechanism.

①将40只大鼠随机等分为正常组、模型组、解扎组、治疗组，以体外直肠不全结扎法造模，以动态浊度法检测各组大鼠血浆内毒素水平，放射免疫分析法检测各组血清TNF-α含量；②大鼠处死后，取左肺进行支气管肺泡灌洗，以TBA法测定支气管肺泡灌洗液中丙二醛含量、白蛋白含量及肺表面活性物质水平；③在红细胞计数盘上作BALF中肺泡巨噬细胞活力计数，计数兰染的细胞数，求出死亡百分率；在光镜下计数BALF沉渣200个细胞，计算中性粒细胞所占百分比。

A monoclonal antibody against nitroaniline was prepared for measuring nitroaniline residuals and for an enzyme linked immuno-sorbent assay reagent kit.

为了测定水中硝基苯胺类化合物，进而开发酶联免疫分析法快速检测方法，制备了抗硝基苯胺单克隆抗体。

The cytotoxicity activity of the whole or nonadherent splenocytes from immunized mice was measured (Hca-F, L929 as target cells) by MTT method.

通过MTT法测定免疫小鼠全脾细胞或不粘附细胞的细胞毒活性(Hca-F、L929为靶细胞)。

Methods The activity of plasma t-PA and PAI were detected by enzyme linked immunosorbent assay respectively in 40 cases with hypertensive disorder complicating pregnancy, 20 nonfertile women and 20 pregnant women.

采用发色底物法和酶联免疫吸附法测定40例妊娠期高血压疾病患者、20例正常非孕妇女及20例正常晚孕妇女血浆组织纤溶酶原激活物及其抑制物活性，并进行比较。

To determine whether there was difference of plasma ANP and/or BNP levels between physiological pacing and nonphysiological pacing with the same daily physical activity scale.

用放射免疫法测定62例起搏患者的血浆ANP、BNP浓度，其中DDD17例，VV139例，VVIR2例，VDD4例，根据特定活动量问卷调查表(specific activity scalequestionnaire)来判断起搏患者的生理活动量，选择Ⅰ级(1组)、Ⅱ级(2组)及Ⅲ级(3组)入组进行研究。

Methods The plasma levels of CGRP in 59 cases of normal pregnant women, 57 cases of PIH, and 10 cases of nonpregnant women were determined with a sensitive radioimmunoassay.

采用放射免疫法对正常妊娠妇女59例、妊高征孕妇57例和正常非孕妇女10例的血浆CGRP水平进行测定。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。