免疫测定

Methods: To detect the spleen index, the thoracic gland index, and erythrocytolysin of the mouse.

运用免疫实验方法检测了正常小鼠空白组和实验组在脾指数、胸腺指数和溶血素水平等方面的测定。

Methods Study the effect of G. lucidum spores oil (at the dose of 2.5,1.25,0.725 g/kg) on reducing the toxicity of the Cy chemotherapy and 60Co radiotherapy by detecting the number of the peripheral blood leucocyte, the number of bone marrow karyota;spleen index, thymus index of normal mice and H22-bearing mice by carbon clearance test and serum erythrocytolysin test. Results It showed that G.

方法以正常和 H22荷瘤小鼠为对象，通过检测外周血 WBC数、骨髓有核细胞数、脾脏指数、胸腺指数来观察口服灵芝孢子油(2.5，1.25，0.625 g/kg)对于环磷酰胺化疗及60Co放疗的减毒作用；通过小鼠碳廓清实验、血清溶血素测定实验检测药物对 H22荷瘤小鼠免疫功能的影响。

Methods Using radioimmunoassay to sufferer hyperinsulinism degree and grouping,compare and analyze course of disease and serious degree of coronary heart disease and pancreatic B-cell excretive function damage.

采用放射免疫方法测定受检者胰岛素抵抗的程度并将其分组，并对比分析其病程与胰岛B细胞分泌功能受损之间的关系和发生冠心病严重程度之间的关系。

In this research, the pubertal five-week-old SD rats were chose as experimental object to establish the animal model for simulating maxillary protraction, on which was imposed the orthopedic force of 85g with utilizing the self-devised maxillary protraction appliance. Located lateral X-ray cephalograms were taken to measure and analyze the changes of maxillary development after exerting the orthopedic force for 4 weeks. The technique of immunohistochemistry was used to investigate theexpression of TGF-, in frontomaxillary sutures and palatomaxillary sutures with identical force performing for different extent of time. The average hue was selected as indicator, which was measured by the means of the analysis system of pathological color images. The measuring data were analyzed by the one-way ANOVA in software SPSS 11.0 version.

本研究采用自行设计的上颌前牵引装置，以生长发育期5周龄SD大鼠为实验对象，施加85g的矫形力，建立了模拟上颌前牵引的动物模型，通过X线头影测量分析，观察施力4周后上颌骨的生长发育情况；应用免疫组织化学技术，检测同一力值作用不同时段下TGF-β_1在上颌骨的额颌缝、腭颌缝的表达，彩色病理图象分析系统测定每张切片中阳性染色细胞的平均灰度值，结果采用SPSS 11.0进行方差分析。

Methods Wistar rats of 6 months old were randomly divided into normal control groupand IR group. Each group had eight rats. Euglycemic insulin clamp technique was used to determine IR and glycose infusion rate was used to IR. Cardiocyte apoptosis was evaluated by TUNEL. Heart weight and body weight were measured to calculate HW/BW. Fine structure of heart and ultramicrostructure of cardiocyte were observed.

6月龄Wistar大鼠，分为正常对照组和IR组，正常血糖胰岛素钳夹技术测定各组大鼠IR，葡萄糖输注速率表示IR情况；测量大鼠心脏重量和体重并计算心脏重量与体重之比；原位末端酶标记法检测各组大鼠心肌细胞凋亡情况；观察心脏微细结构和心肌细胞超微结构；免疫组化检测心脏间质Ⅰ、Ⅲ型胶原水平。

Objective To explore the regulation effect of haematoxylin on immunogenic reaction of rats suffered from UC by serum TLC subgroup, IgG and lysozyme level examination.

目的 通过对溃疡性结肠炎大鼠的血清TLC亚群的检测和IgG、溶菌酶含量的测定，探讨苏木对UC大鼠免疫功能的调节作用。

Real-time quantitative pcr, immunohistochemistry, dry-wet weight method, histological techniques and haematoxylin and eosin stain were used to detect expression of icam-1, the change of mrna at different time phases, water containing in the brain tissue and the inflammatory infiltration respectively after diffuse brain injury.

采用marmarou方法获得大鼠弥漫性脑损伤模型，实时定量rt-pcr、s-p免疫组化法分别测定icam-1蛋白和mrna在外伤后不同时间点的表达变化，干湿重法测脑组织含水量，组织切片苏木精-伊红染色观测炎性细胞浸润情况。

Methods The rat models of diffuse brain injury were established by Marmarou's method. Real-time quantitative PCR, immunohistochemistry, dry-wet weight method, histological techniques and haematoxylin and eosin stain were used to detect expression of ICAM-1, the change of mRNA at different time phases, water containing in the brain tissue and the inflammatory infiltration respectively after diffuse brain injury.

采用Marmarou方法获得大鼠弥漫性脑损伤模型，实时定量RT-PCR、S-P免疫组化法分别测定ICAM-1蛋白和mRNA在外伤后不同时间点的表达变化，干湿重法测脑组织含水量，组织切片苏木精-伊红染色观测炎性细胞浸润情况。

Methods:Thyroid function,thyroid microsome antibody,and thyroid globulin antibody were determined with radioimmunoassay in 23 CRF complicated by primary hypothyroidism patients,and the serum calcium,phosphorus,potassium, cholesterol, glucose, urea nitrogen, hemoglobulin,and endogenous creatinine clearance rate were also assayed.

用放射免疫法测定23例CRF合并甲减患者的甲状腺功能、甲状腺微粒体抗体、甲状腺球蛋白抗体及检测血清钙、血清磷、血清钾、血清胆固醇、血清肌酐、血糖、尿素氮、血红蛋白、内生肌酐清除率并与31例单纯CRF患者对照进行分析。

Methods 90 Sprague-Daw rats were divided into sham-operative group, ICH model group and ginkgo therapy group. Every group divided 6h, 12h, 24h, 72h and 7d. Collagenase type Ⅶ with heparinize was infused into the right caudate nucleus to establish rat experimental ICH model.

采用肝素化VII型胶原酶脑内注入法制作大鼠脑出血模型，应用免疫组化技术测定大鼠脑出血后血肿周围组织中IL-8、ICAM-1的表达含量。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷